Recombinant Human Neuroglycan C/CSPG5 Protein, CF

Catalog # Availability Size / Price Qty
5685-NG-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human Neuroglycan C/CSPG5 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit Recombinant Human Midkine (Catalog # 258-MD) induced neurite outgrowth in E16-E18 rat embryonic cortical neurons. When 5 x 104 neurons per well are added to a plate pre-incubated with serial dilutions of rhNeuroglycan-C and 1 μg/mL of rhMidkine, the neurite outgrowth promotion is significantly inhibited in a dose dependent manner with an effect at
0.6-1.2 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Neuroglycan C/CSPG5 protein
Val31-Gln420, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Val31
Predicted Molecular Mass

41.9 kDa

SDS-PAGE
80-100 kDa, reducing conditions

Product Datasheets

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5685-NG

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5685-NG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Neuroglycan C/CSPG5

Neuroglycan C (NGC; also CSPG5 and CALEB) is a 120 - 150 kDa type I transmembrane glycoprotein and member of the neuregulin family of proteins (1 ‑ 2). Depending on its expression profile, NGC may be a glycoprotein of 120 kDa, or a chondroitin sulfate (CS) proteoglycan of 150 kDa (2 - 3). Human NGC is synthesized as a 566 amino acid (aa) precursor that contains a 30 aa signal sequence, a 393 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 122 aa cytoplasmic region. The ECD contains one CS attachment domain (aa 34 - 272), with CS attachment at Ser117, one EGF-like domain (aa 371 - 413), two potential sites for N-linked glycosylation, and twelve potential sites for O-linked glycosylation (4). Splicing variants produce three isoforms for human NGC. Isoform 1 is the long form. Isoform 2 has a deletion of aa 487 ‑ 513, while isoform 3 has an alternative start site at Met139 and the same deletion. Phosphorylation likely occurs at Ser249, and proteolysis generates a 75 kDa soluble fragment (5). Over aa 31 - 420, human NGC shares 84% aa identity with mouse NGC. NGC is expressed in nervous tissue and is found on retinal ganglion cells, cerebellar Purkinje cells and hippocampal neurons (6). NGC may function as a growth and differentiation factor involved in neuritogenesis. One study shows that the recombinant ectodomain of NGC core protein enhances neurite outgrowth from rat neocortical neurons in culture via phosphatidylinositol 3-kinase and protein kinase C signaling pathways (7). Another study states that NGC is a novel component of midkine receptors, a heparin-binding growth factor that promotes cell attachment and process extension in oligodendroglial precursor-like cells (3). NGC also acts as a growth factor by directly binding ERbB3 tyrosine kinase and transactivating ErbB2 (1).

References
  1. Kinugasa, Y. et al. (2004) Biochem. Biophys. Res. Commun 321:1045.
  2. Yasuda, Y. et al. (1998) Neurosci. Res. 32:313.
  3. Ichihara-Tanaka, K. et al. (2006) J. Biol. Chem. 281:30857.
  4. Aono, S. et al. (2004) J. Biol. Chem. 279:46536.
  5. Shuo, T. et al. (2007) J. Neurochem. 102:1561.
  6. Aono, S. et al. (2006) J. Neurosci. Res.83:110.
  7. Nakanishi, K. et al. (2006) J. Biol. Chem. 281:24970.

Entrez Gene IDs
10675 (Human); 29873 (Mouse); 50568 (Rat)
Alternate Names
CALEB; chondroitin sulfate proteoglycan 5 (neuroglycan C); chondroitin sulfate proteoglycan 5; CSPG5; MGC44034; Neuroglycan C; NGC; NGCAcidic leucine-rich EGF-like domain-containing brain protein

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Recombinant Human Neuroglycan C/CSPG5 Protein, CF
By Takuya Shuo on 06/23/2020
Application: SDS-PAGE Control