Recombinant Human Neuroglycan C/CSPG5 Protein, CF

R&D Systems | Catalog # 5685-NG

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems CHO-derived Recombinant Human Neuroglycan C/CSPG5 Protein (5685-NG)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

AAQ04774

Applications

Bioactivity
View all Neuroglycan C/CSPG5 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Neuroglycan C/CSPG5 protein
Val31-Gln420, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val31

Predicted Molecular Mass

41.9 kDa

SDS-PAGE

80-100 kDa, reducing conditions

Activity

Bioassay data are not available.

Reviewed Applications

Read 1 review rated 5 using 5685-NG in the following applications:

Formulation, Preparation, and Storage

5685-NG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Neuroglycan C/CSPG5

Neuroglycan C (NGC; also CSPG5 and CALEB) is a 120 - 150 kDa type I transmembrane glycoprotein and member of the neuregulin family of proteins (1‑2). Depending on its expression profile, NGC may be a glycoprotein of 120 kDa, or a chondroitin sulfate (CS) proteoglycan of 150 kDa (2-3). Human NGC is synthesized as a 566 amino acid (aa) precursor that contains a 30 aa signal sequence, a 393 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 122 aa cytoplasmic region. The ECD contains one CS attachment domain (aa 34-272), with CS attachment at Ser117, one EGF-like domain (aa 371-413), two potential sites for N-linked glycosylation, and twelve potential sites for O-linked glycosylation (4). Splicing variants produce three isoforms for human NGC. Isoform 1 is the long form. Isoform 2 has a deletion of aa 487‑513, while isoform 3 has an alternative start site at Met139 and the same deletion. Phosphorylation likely occurs at Ser249, and proteolysis generates a 75 kDa soluble fragment (5). Over aa 31-420, human NGC shares 84% aa identity with mouse NGC. NGC is expressed in nervous tissue and is found on retinal ganglion cells, cerebellar Purkinje cells and hippocampal neurons (6). NGC may function as a growth and differentiation factor involved in neuritogenesis. One study shows that the recombinant ectodomain of NGC core protein enhances neurite outgrowth from rat neocortical neurons in culture via phosphatidylinositol 3-kinase and protein kinase C signaling pathways (7). Another study states that NGC is a novel component of midkine receptors, a heparin-binding growth factor that promotes cell attachment and process extension in oligodendroglial precursor-like cells (3). NGC also acts as a growth factor by directly binding ERbB3 tyrosine kinase and transactivating ErbB2 (1).

References

  1. Kinugasa, Y. et al. (2004) Biochem. Biophys. Res. Commun 321:1045.
  2. Yasuda, Y. et al. (1998) Neurosci. Res. 32:313.
  3. Ichihara-Tanaka, K. et al. (2006) J. Biol. Chem. 281:30857.
  4. Aono, S. et al. (2004) J. Biol. Chem. 279:46536.
  5. Shuo, T. et al. (2007) J. Neurochem. 102:1561.
  6. Aono, S. et al. (2006) J. Neurosci. Res.83:110.
  7. Nakanishi, K. et al. (2006) J. Biol. Chem. 281:24970.

Alternate Names

CALEB, CSPG5, NGC

Entrez Gene IDs

10675 (Human); 29873 (Mouse); 50568 (Rat)

Gene Symbol

CSPG5

UniProt

AAQ04774

Additional Neuroglycan C/CSPG5 Products

Product Documents for Recombinant Human Neuroglycan C/CSPG5 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human Neuroglycan C/CSPG5 Protein, CF

For research use only

Related Research Areas

Glial Lineage Markers
Proteoglycans

Customer Reviews for Recombinant Human Neuroglycan C/CSPG5 Protein, CF (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Recombinant Human Neuroglycan C/CSPG5 Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Recombinant Human Neuroglycan C/CSPG5 Protein, CF
    Name: Takuya Shuo
    Application: SDS-PAGE Control
    Verified Customer | Posted 06/23/2020
    Recombinant Human Neuroglycan C/CSPG5 Protein, CF 5685-NG

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes