Recombinant Human Neuronal Pentraxin 2 Protein, CF

Catalog # Availability Size / Price Qty
7816-NP-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human Neuronal Pentraxin 2 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of TT human medullary thyroid cancer cells. The ED50 for this effect is 0.6‑2.4 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human Neuronal Pentraxin 2 protein
Gly16-Leu431, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Gly16
Predicted Molecular Mass
46.5 kDa
SDS-PAGE
50-60 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7816-NP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Neuronal Pentraxin 2

Neuronal Pentraxin (NPTX2), also called NP2 or NARP (neuronal activity-regulated pentraxin) is a 46-60 kDa secreted glycoprotein within the Pentraxin family (1-4). Neuronal pentraxins include NPTX1 and NPTX2, both secreted proteins, and NPTXR (Neuronal Pentraxin Receptor), which is found in type II transmembrane or cleaved, soluble forms (1-5). Circulating NPTX2 forms disulfide-linked 250 kDa homopentamers, while at excitatory synapses it can form heteropentamers and larger clusters with NPTX1 and NPTXR (1-5). The clusters promote synaptogenesis by recruiting subunits of AMPA-type glutamate receptors (AMPAR) (4-6). Of the three neuronal pentraxins, only NPTX2 shows immediate-early expression induced by synaptic activity (5, 6). Human NPTX2 is synthesized as a 431 amino acid (aa) protein with a 15 aa signal sequence and a 416 aa secreted mature protein that contains a calcium binding Pentraxin domain. Mature human NPTX2 shares 96% aa sequence identity with mouse, rat, bovine, and canine NPTX2. Unlike other neuronal pentraxins, NPTX2 expression is not limited to neurons and NPTX2 protein is detected in the plasma (1, 7). It is prominent in the hippocampus, cerebral cortex, cerebellum, hypothalamus, posterior pituitary, and retina (2-8). It is found at excitatory synapses in parvalbumin-expressing interneurons and vasopressin- and orexin-expressing hypothalamic neurons (5-7). Its expression is increased in the substantia nigra in Parkinson’s disease, and it is present in Lewy bodies (9). NPTX2 shows calcium-dependent adhesion (3, 6). It promotes axon outgrowth in cortical explants, and formation, maturation and plasticity of synapses in vivo (3-6, 8).

References
  1. Hsu, Y.C. and M.S. Perin (1995) Genomics 28:220.
  2. Kirkpatrick, L.L. et al. (2000) J. Biol. Chem. 275:17786.
  3. Tsui, C.C. et al. (1996) J. Neurosci. 16:2463.
  4. Cho, R.W. et al. (2008) Neuron 57:858.
  5. Xu, D. et al. (2003) Neuron 39:513.
  6. Chang, M.C. et al. (2010) Nat. Neurosci. 13:1090.
  7. Reti, I.M. et al. (2008) Neuroscience 151:352.
  8. Bjartmar, L. et al. (2006) J. Neurosci. 26:6269.
  9. Moran, L.B. et al. (2008) Acta Neuropathol. 115:471.
Entrez Gene IDs
4885 (Human); 53324 (Mouse); 288475 (Rat)
Alternate Names
Apexin; NARP; neuronal activity-regulated pentaxin; Neuronal Pentraxin 2; neuronal pentraxin IIapexin; neuronal pentraxin-2; NP2; NP-II; NPTX2

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