Recombinant Human Olfactomedin-2/Noelin-2 Protein, CF

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5614-NL-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human Olfactomedin-2/Noelin-2 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of HEK293T human embryonic kidney cells.

When 5 x 104 cells per well are added to human Olfactomedin-2 coated plates (1 μg/mL, 100 μL/well), approximately 50%-75% will adhere after 1 hour at 37 °C.

Optimal dilutions should be determined by each laboratory for each application.

Source
Chinese Hamster Ovary cell line, CHO-derived human Olfactomedin-2/Noelin-2 protein
Gln21-Pro454, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
No results obtained: Gln21 predicted
Predicted Molecular Mass
50 kDa
SDS-PAGE
55-75 kDa, reducing conditions

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5614-NL

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5614-NL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Olfactomedin-2/Noelin-2

Olfactomedin-2 (OLFM2, OM2), also called OlfC or neuronal olfactomedin-related endoplasmic reticulum-localized-2 (Noelin-2) is a 50 kDa (predicted), secreted member of the olfactomedin/noelin family of proteins (1 - 3). Since Noelin-2 has also been used to designate an alternately spliced form of Noelin-1, Olfactomedin-2 is the less ambiguous name (4). Olfactomedin domain-containing proteins are often found in neural tissues (3). Human Olfactomedin-2 expressed sequence tags have mainly been found in the brain and eye, and RNA in the cerebellum (1, 2). In zebrafish, Olfactomedin-2 expression is developmentally regulated and, like Noelin-1, may play a role in neural crest cell differentiation (5). The human Olfactomedin-2 cDNA encodes a 20 amino acid (aa) signal sequence and a 454 mature protein that contains two coiled-coil regions (aa 58 - 85 and 136 - 193) and an Olfactomedin domain (aa 194 - 446). Mature human Olfactomedin-2 shares 98% aa identity with rat, bovine and equine, 97% with mouse and canine, 81% with Xenopus, and 77% with zebrafish Olfactomedin-2. No alternate splice variants have been reported for Olfactomedin-2 (1). Polymorphisms of human Olfactomedin-2, with or without additional polymorphisms in optineurin (OPTN), have been associated with a small fraction of high intraocular pressure or open-angle glaucoma cases in Japanese patients (6). The family member myocilin shows stronger association with these ocular disorders, and either myocilin or Noelin-1 might heterodimerize with Olfactomedin-2 (1). Evidence suggests that Olfactomedin-2 has evolved from Noelin-1 gene duplication, and myocilin from Olfactomedin-2 gene duplication (1).

References
  1. Mukhopadhyay, A. et al. (2004) Mol. Vis. 10:304.
  2. Kulkarni, N.H. et al. (2000) Genet. Res. Camb. 76:41.
  3. Tomarev, S.I. and N. Nakaya (2009) Mol. Neurobiol. 40:122.
  4. Moreno, T.A. & M. Bronner-Fraser (2002) Mech. Dev. 119:121.
  5. Lee, J-A. et al. (2008) Mech. Dev. 125:167.
  6. Funayama, T. et al. (2006) Invest. Ophthalmol. Vis. Sci. 47:5368.
Entrez Gene IDs
93145 (Human); 244723 (Mouse); 313783 (Rat)
Alternate Names
neuronal olfactomedin related ER localized protein 2; NOE2; NOE2noelin 2; NOELIN2; Noelin-2; NOELIN2_V1; olfactomedin 2; Olfactomedin2; Olfactomedin-2; OlfC; OLFM2; OM2

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