Recombinant Human Osteocrin Protein, CF

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Recombinant Human Osteocrin Protein Binding Activity
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Product Details
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Recombinant Human Osteocrin Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Human NPRC/NPR3 Fc Chimera (Catalog # 10233-NR) is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human Osteocrin that produces 50% of the optimal binding response is 2-20 ng/mL.
E. coli-derived human Osteocrin protein
Val28-Gly133, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
13 kDa
18 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Human Osteocrin Protein Binding Activity View Larger

When Recombinant Human NPRC/NPR-3 Fc Chimera (Catalog # 10233-NR) is immobilized at 2 µg/mL, Recombinant Human Osteocrin (Catalog # 9669-ON) binds with an ED50 of 2-20 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: Osteocrin

Osteocrin, also known as Musclin, is a secreted protein that is primarily expressed in bone and muscle. It is synthesized as a proprotein (11 kDa) that undergoes proteolytic processing to generate a mature 50 amino acids (5 kDa) C-terminal peptide (1). It was found to modulate osteoblast differentiation and to regulate glucose metabolism in muscles (2). Human Osteocrin proprotein shares 77% and 78% amino acid sequence identity with the rat and mouse protein, respectively. Secretion of Osteocrin has been shown to be increased with exercise and is associated with metabolically beneficial formation of brown fat (3). Osterocrin has also been attributed to increases in exercise endurance through promotion of mitochondrial biosynthesis (4). Based on similarities with NPs (natriuretic peptides), Osteocrin was found to interact with NP clearance receptor NPR-C. This interaction is thought to modulate the availability of NPs, importantly increasing CNP which has been shown to stimulate endochondral ossification and elongate bones (5). In primates, Osteocrin may have evolved to regulate neuronal structure and function (6).

  1. Nishizawa, H. et al. (2004) J Bio Chem 279:19391.
  2. Thomas, G. et al. (2003) J Bio Chem 278:50563.
  3. Jeramic, N. et al. (2017) J Cell Physiol 232:61.
  4. Subbatino, E. et al. (2015) Proc Natl Acad Sci U S A 112:16042.
  5. Kanai, Y. et al. (2017) J of Clin Invest 127:4136.
  6. Ataman, B. et al. (2016) Nature 539:242.
Entrez Gene IDs
344901 (Human); 239790 (Mouse)
Alternate Names
Musclin; Osteocrin; OSTN

Citation for Recombinant Human Osteocrin Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Osteocrin, a novel myokine, prevents diabetic cardiomyopathy via restoring proteasomal activity
    Authors: X Zhang, C Hu, XP Yuan, YP Yuan, P Song, CY Kong, T Teng, M Hu, SC Xu, ZG Ma, QZ Tang
    Cell Death & Disease, 2021-06-16;12(7):624.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo


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