Recombinant Human Osteocrin Protein, CF

R&D Systems | Catalog # 9669-ON

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Key Product Details

  • R&D Systems E. coli-derived Recombinant Human Osteocrin Protein (9669-ON)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

P61366

Applications

Bioactivity
View all Osteocrin Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Osteocrin protein
Val28-Gly133, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val28

Predicted Molecular Mass

13 kDa

SDS-PAGE

18 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human NPRC/NPR3 Fc Chimera (Catalog # 10233-NR) is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human Osteocrin that produces 50% of the optimal binding response is 2-20 ng/mL.

Scientific Data Images for Recombinant Human Osteocrin Protein, CF

Recombinant Human Osteocrin Protein Binding Activity

Recombinant Human Osteocrin Protein Binding Activity

When Recombinant Human NPRC/NPR-3 Fc Chimera (Catalog # 10233-NR) is immobilized at 2 µg/mL, Recombinant Human Osteocrin (Catalog # 9669-ON) binds with an ED50 of 2-20 ng/mL.

Formulation, Preparation, and Storage

9669-ON
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Osteocrin

Osteocrin, also known as Musclin, is a secreted protein that is primarily expressed in bone and muscle. It is synthesized as a proprotein (11 kDa) that undergoes proteolytic processing to generate a mature 50 amino acids (5 kDa) C-terminal peptide (1). It was found to modulate osteoblast differentiation and to regulate glucose metabolism in muscles (2). Human Osteocrin proprotein shares 77% and 78% amino acid sequence identity with the rat and mouse protein, respectively. Secretion of Osteocrin has been shown to be increased with exercise and is associated with metabolically beneficial formation of brown fat (3). Osterocrin has also been attributed to increases in exercise endurance through promotion of mitochondrial biosynthesis (4). Based on similarities with NPs (natriuretic peptides), Osteocrin was found to interact with NP clearance receptor NPR-C. This interaction is thought to modulate the availability of NPs, importantly increasing CNP which has been shown to stimulate endochondral ossification and elongate bones (5). In primates, Osteocrin may have evolved to regulate neuronal structure and function (6).

References

  1. Nishizawa, H. et al. (2004) J Bio Chem 279:19391.
  2. Thomas, G. et al. (2003) J Bio Chem 278:50563.
  3. Jeramic, N. et al. (2017) J Cell Physiol 232:61.
  4. Subbatino, E. et al. (2015) Proc Natl Acad Sci U S A 112:16042.
  5. Kanai, Y. et al. (2017) J of Clin Invest 127:4136.
  6. Ataman, B. et al. (2016) Nature 539:242.

Alternate Names

Musclin, OSTN

Entrez Gene IDs

344901 (Human); 239790 (Mouse)

Gene Symbol

OSTN

UniProt

P61366

Additional Osteocrin Products

Product Documents for Recombinant Human Osteocrin Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Osteocrin Protein, CF

For research use only

Citations for Recombinant Human Osteocrin Protein, CF

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