Recombinant Human Pentraxin 3/TSG-14 Protein

Formulations:
Catalog # Availability Size / Price Qty
1826-TS-025
Product Details
Citations (10)
FAQs
Reviews (1)

Recombinant Human Pentraxin 3/TSG-14 Protein Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized rhC1q at 5 µg/mL (100 µL/well) can bind rhPentraxin 3 with a linear range of 0.08-5 µg/mL.
Source
Mouse myeloma cell line, NS0-derived human Pentraxin 3/TSG-14 protein
Glu18-Ser381, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Glu18
Structure / Form
Multimer consisting of as many as ten non-covalently and covalently linked subunits
Predicted Molecular Mass
41 kDa (monomer)
SDS-PAGE
41-50 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1826-TS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1826-TS/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Pentraxin 3/TSG-14

Pentraxin 3 (PTX3), TSG-14, was initially identified as a TNF-alpha or IL-1 beta inducible gene (1-3). It belongs to the pentraxin family, which was named originally for the homo-pentameric structure formed by its members (4). The pentraxin family is divided into two subfamilies: the “short” and the “long” pentraxins with approximate molecular weights of 25 kDa and 50 kDa, respectively. TSG-14 is a member of the long pentraxin subfamily, which also includes the Xenopus laevis XL-PXN1, the guinea pig apexin/p50, the rat neuronal pentraxin I (NPI) and NPR, the human neuronal pentraxin II (NPTX2) and the human neuronal activity-related pentraxin (5).

Mature secreted TSG-14 contains a pentaxin-like domain at its carboxy-terminus that shares 23-28% amino acid (aa) sequence similarity to C-reactive protein (CRP) and serum amyloid P component (SAP), which belongs to the short pentraxin subfamily. However, the N-terminal sequence of TSG-14 does not share aa sequence homology with any of the “short” pentaxins (3). Unlike CRP and SAP, which forms pentamers only, TSG-14 forms both pentameric and higher ordered oligomers (5). Similarly to CRP and SAP, TSG-14 binds to the complement cascade component C1q (6). However, TSG-14 does not bind to phosphoethanolamine, phosphocholine, or high pyruvate agarose, which are known ligands for CRP and SAP. TSG-14 is a marker of the acute phase response and is highly expressed in advanced atherosclerotic plaques (12). While CRP and SAP are primarily produced in the liver, TSG-14 expression is strongly upregulated by TNF-alpha, IL-1 beta, and bacterial LPS in peripheral fibroblasts, endothelial cells, and macrophages (7). At the amino acid level, human and mouse TSG-14 share 88% aa sequence homology (8). TSG-14 concentration is elevated in the joint fluid of patients with rheumatoid arthritis (RA), indicating that TSG-14 may be a potential mediator of immune response (9).
TSG-14 may also function in the regulation of the uptake and clearance of apoptotic cells by dendritic cells (10). In vivo study showed that TSG-14 transgenic mice are more resistant to sepsis and endotoxemia compared to wild type during the inflammatory injury (11). Increased expression of TSG-14 may enhance the immune response to protect the host from infection.

References
  1. Lee, T.H. et al. (1990) Mol. Cell. Biol. 10:1982.
  2. Breviario, F. et al. (1992) J. Biol. Chem. 267:22190.
  3. Lee, G.W. et al. (1993) J. Immunol. 150:1804.
  4. Osmand, A.P. et al. (1977) Proc. Natl. Acad. Sci. USA 74:739.
  5. Goodman A.R. et al. (1996) Cytokine & Growth Factor Reviews 7:191.
  6. Bottazzi, B. et al. (1997) J. Biol. Chem. 272:32817.
  7. Introna, M. et al. (1996) Blood 87:1862.
  8. Altmeyer, A. et al. (1995) J. Biol. Chem. 270:25584.
  9. Luchetti, M.M. et al. (2000) Clin. Exp. Immunol. 119:196.
  10. Rovere, P. et al. (2000) Blood 96:4300.
  11. Dias, A.A.M. et al. (2001) J. Leukocyte Biol. 69:928.
  12. Rolph, M.S. et al. (2002) Arterioscler. Throm. Vasc. Biol. 22:e10-4.
Long Name
Tumor Necrosis Factor Stimulated Gene 14
Entrez Gene IDs
5806 (Human); 19288 (Mouse)
Alternate Names
alpha-induced protein 5; pentaxin-related gene, rapidly induced by IL-1 beta, tumor necrosis factor; Pentaxin-related protein PTX3; Pentraxin 3; pentraxin 3, long; pentraxin-3; pentraxin-related gene, rapidly induced by IL-1 beta; pentraxin-related protein PTX3; PTX3; TNF alpha-induced protein 5; TNFAIP5; TSG14; TSG-14; TSG-14pentaxin-related gene, rapidly induced by IL-1 beta; Tumor necrosis factor alpha-induced protein 5; tumor necrosis factor, alpha-induced protein 5; Tumor necrosis factor-inducible gene 14 protein; tumor necrosis factor-inducible protein TSG-14

Citations for Recombinant Human Pentraxin 3/TSG-14 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. FHR-1 Binds to C-Reactive Protein and Enhances Rather than Inhibits Complement Activation
    Authors: ÁI Csincsi, Z Szabó, Z Bánlaki, B Uzonyi, M Cserhalmi, É Kárpáti, A Tortajada, JJE Caesar, Z Prohászka, TS Jokiranta, SM Lea, S Rodríguez, M Józsi
    J. Immunol., 2017;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. Monocyte differentiation and macrophage priming are regulated differentially by pentraxins and their ligands
    Authors: D Pilling, E Galvis-Car, TR Karhadkar, N Cox, RH Gomer
    BMC Immunol., 2017;18(1):30.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Three pentraxins C-reactive protein, serum amyloid p component and pentraxin 3 mediate complement activation using Collectin CL-P1
    Authors: Nobutaka Wakamiya
    Biochim. Biophys. Acta, 2016;0(0):.
  4. Role of pentraxin 3 in shaping arthritogenic alphaviral disease: from enhanced viral replication to immunomodulation.
    Authors: Foo, Suan-Sin, Chen, Weiqiang, Taylor, Adam, Sheng, Kuo-Chin, Yu, Xing, Teng, Terk-Shi, Reading, Patrick, Blanchard, Helen, Garlanda, Cecilia, Mantovani, Alberto, Ng, Lisa F P, Herrero, Lara J, Mahalingam, Suresh
    PLoS Pathog, 2015;11(2):e1004649.
    Species: Human
    Sample Types: Virus
    Applications: Bioassay
  5. Factor H-related protein 5 interacts with pentraxin 3 and the extracellular matrix and modulates complement activation.
    Authors: Csincsi A, Kopp A, Zoldi M, Banlaki Z, Uzonyi B, Hebecker M, Caesar J, Pickering M, Daigo K, Hamakubo T, Lea S, Goicoechea de Jorge E, Jozsi M
    J Immunol, 2015;194(10):4963-73.
    Species: Human
    Sample Types: Protein
    Applications: Binding Assay
  6. The long pentraxin PTX3 promotes fibrocyte differentiation.
    Authors: Pilling D, Cox N, Vakil V, Verbeek J, Gomer R
    PLoS ONE, 2015;10(3):e0119709.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Pentraxin 3 (PTX3) expression in allergic asthmatic airways: role in airway smooth muscle migration and chemokine production.
    Authors: Zhang J, Shan L, Koussih L, Redhu NS, Halayko AJ, Chakir J, Gounni AS
    PLoS ONE, 2012;7(4):e34965.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Atypical hemolytic uremic syndrome-associated variants and autoantibodies impair binding of factor h and factor h-related protein 1 to pentraxin 3.
    J. Immunol., 2012;189(4):1858-67.
    Species: Human
    Sample Types: Protein
    Applications: Binding Assay
  10. Pharmacoproteomics of a metalloproteinase hydroxamate inhibitor in breast cancer cells: dynamics of membrane type 1 matrix metalloproteinase-mediated membrane protein shedding.
    Authors: Butler GS, Dean RA, Tam EM, Overall CM
    Mol. Cell. Biol., 2008;28(15):4896-914.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Human Pentraxin 3/TSG-14 Protein
By Anonymous on 12/01/2017
Application: Binding assay/Protein-protein interaction