Recombinant Human Pleiotrophin/PTN Protein, CF

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R&D Systems Recombinant Proteins and Enzymes
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Citations (5)
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Recombinant Human Pleiotrophin/PTN Protein, CF Summary

Product Specifications

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cerebral cortical neurons. Muramatsu, H. and T. Muramatsu (1991) Biochem. Biophys. Res. Commu. 177:652. Optimal neurite outgrowth was observed when neurons were plated on 96 well culture plates that had been pre-coated with 100 µL/well of a solution of 3‑8 µg/mL rhPTN.
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Pleiotrophin/PTN protein
Accession #
N-terminal Sequence
Predicted Molecular Mass
15.3 kDa
18 kDa, reducing conditions

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Pleiotrophin/PTN

PTN was identified independently by several groups as a novel heparin-binding, developmentally regulated cytokine. Depending on the biological activities studied, this protein has variously been referred to as heparin-binding brain mitogen (HBBM), heparin-binding growth factor-8 (HBGF-8), heparin-binding neurite promoting factor, heparin-binding neurotrophic factor (HBNF), heparin-affinity regulatory peptide (HARP), heparin-binding growth-associated molecule (HB-GAM), osteoblast-specific factor (OSF-1), and pleiotrophin. PTN is a highly conserved protein; the amino acid sequences of human, bovine, rat, and mouse PTN share > 98% homology.

PTN is a member of a family of heparin-binding proteins that share sequence, structural, and functional similarity. Other members of this family include midkine (MK), and chicken retinoic acid-induced heparin-binding protein (RI-HB), an avian homologue of MK. The expression of all these cytokines is restricted and highly regulated during development.

PTN can be used as an attachment substrate to stimulate neurite outgrowth in mixed cultures of embryonic rat, mouse or chicken brain cells. Although both natural and recombinant human PTN have been reported to be mitogenic for fibroblasts, endothelial, and epithelial cells, the data are still highly controversial. The insect cell-derived recombinant PTN preparations produced at R&D Systems lack mitogenic activities when tested on various fibroblast, endothelial, and epithelial cell lines. PTN has been shown to transform NIH-3T3 and SW-13 cells, as evidenced by anchorage-independent growth and tumor formation in the nude mouse. These results suggest that, in spite of the conflicting reports of PTN’s growth-promoting activity in vitro, PTN may have a role in abnormal cell growth in vivo.

Entrez Gene IDs
5764 (Human)
Alternate Names
HARP; HBBM; HB-GAM; HBGF8; HBNF; HBNF1; HBNF-1; Heparin-binding brain mitogen; HNGF-8; NEGF1HBGF-8; neurite growth-promoting factor 1; neurite growth-promoting factor1); OSF-1; Osteoblast-specific factor 1; Pleiotrophin; PTN

Citations for Recombinant Human Pleiotrophin/PTN Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Discovery of PTN as a serum-based biomarker of pro-metastatic prostate cancer
    Authors: S Liu, M Shen, EC Hsu, CA Zhang, F Garcia-Mar, R Nolley, K Koul, MA Rice, M Aslan, SJ Pitteri, C Massie, A George, JD Brooks, VJ Gnanapraga, T Stoyanova
    British Journal of Cancer, 2020;0(0):.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: ELISA Standard
  2. A glucocorticoid- and diet-responsive pathway toggles adipocyte precursor cell activity in vivo.
    Authors: Wong J, Krueger K, Costa M, Aggarwal A, Du H, McLaughlin T, Feldman B
    Sci Signal, 2016;9(451):ra103.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Receptor-type protein-tyrosine phosphatase zeta is a functional receptor for interleukin-34.
    Authors: Nandi S, Cioce M, Yeung Y, Nieves E, Tesfa L, Lin H, Hsu A, Halenbeck R, Cheng H, Gokhan S, Mehler M, Stanley E
    J Biol Chem, 2013;288(30):21972-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  4. Pleiotrophin produced by multiple myeloma induces transdifferentiation of monocytes into vascular endothelial cells: a novel mechanism of tumor-induced vasculogenesis.
    Authors: Chen H, Campbell RA, Chang Y, Li M, Wang CS, Li J, Sanchez E, Share M, Steinberg J, Berenson A, Shalitin D, Zeng Z, Gui D, Perez-Pinera P, Berenson RJ, Said J, Bonavida B, Deuel TF, Berenson JR
    Blood, 2009;113(9):1992-2002.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Pleiotrophin is highly expressed by myeloma cells and promotes myeloma tumor growth.
    Authors: Chen H, Gordon MS, Campbell RA, Li M, Wang CS, Lee HJ, Sanchez E, Manyak SJ, Gui D, Shalitin D, Said J, Chang Y, Deuel TF, Baritaki S, Bonavida B, Berenson JR
    Blood, 2007;110(1):287-95.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)


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