Recombinant Human Plexin C1 Protein, CF Summary
Ala35-Thr944, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: Plexin C1
Plexin C1, also known as Virus-encoded semaphorin protein receptor (VESPR) and CD232, is a 210 kDa type I transmembrane glycoprotein in the C subfamily of the Plexin family (1, 2). Human Plexin C1 contains a 34 amino acid (aa) signal sequence, a 910 aa extracellular domain (ECD) with one Sema-domain and two cysteine-rich Met-related sequences (MRS), a 21 aa transmembrane domain and a 603 aa cytoplasmic domain that includes features common to other plexins, including a phosphothreonine site. The human Plexin C1 ECD shares 85%, 82% and 71% aa identity with murine, bovine and opossum Plexin C1, respectively. Plexin C1 is widely expressed in neuronal and non-neuronal fetal and adult tissues (3). In neuronal development, its role is unclear. Semaphorin-7a (Sema 7A, CD108) binds Plexin C1 in vitro, and the two show a similar expression pattern during rat neuronal development. However, in rat, beta 1 integrins rather than Plexin C1 appear to mediate Sema 7A effects on axon outgrowth (4, 5). Plexin C1 does appear to play a role in the partitioning of paraventricular and supraoptic neurons in the hypothalamus, as indicated by specific defects seen in mice deleted for Plexin C1 (6). In the immune system, effects of Sema 7A on T cell-mediated inflammatory responses also appear to be mediated by beta 1 integrins rather than plexins (7). However, Plexin C1 may function to oppose the effect of beta 1 integrins, as it does on Sema 7A-mediated spreading and dendrite formation in melanocytes (8). Plexin C1 is the receptor for the poxvirus (A39R protein) and herpes virus (AHVsema) semaphorin homologs, and mediates activation of monocytes and inhibition of dendritic cell and neutrophil phagocytosis by A39R (2, 9, 10).
- Negishi, M. et al. (2005) Cell. Mol. Life Sci. 62:1363.
- Comeau, M.R. et al. (1998) Immunity 8:473.
- Perala, N.M. et al. (2005) Gene Expr. Patterns 5:355.
- Pasterkamp, R.J. et al. (2007) BMC Dev. Biol. 7:98.
- Pasterkamp, R.J. et al. (2003) Nature 424:398.
- Xu, C. and C-M. Fan (2007) Mol. Endocrinol. 21:1234.
- Suzuki, K. et al. (2007) Nature 446:680.
- Scott, G.A. et al. (2007) J. Invest. Dermatol. 128:151.
- Walzer, T. et al. (2005) J. Immunol. 174:51.
- Walzer, T. et al. (2005) Eur. J. Immunol. 35:391.
Citations for Recombinant Human Plexin C1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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Endogenous Semaphorin-7A Impedes Human Lung Fibroblast Differentiation
Authors: S Esnault, EE Torr, K Bernau, MW Johansson, EA Kelly, N Sandbo, NN Jarjour
PLoS ONE, 2017;12(1):e0170207.
Sample Types: Whole Cells
Essential Mechanisms of Differential Activation of Eosinophils by IL-3 Compared to GM-CSF and IL-5
Authors: S Esnault, EA Kelly
Crit. Rev. Immunol., 2016;36(5):429-444.
Sample Types: Whole Cells
Profiling mRNA of the graying human hair follicle constitutes a promising state-of-the-art tool to assess its aging: an exemplary report.
Authors: Peters E, Liezmann C, Spatz K, Ungethum U, Kuban R, Daniltchenko M, Kruse J, Imfeld D, Klapp B, Campiche R
J Invest Dermatol, 2013;133(5):1150-60.
Sample Types: Whole Tissue
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