Recombinant Human PlGF-2 Protein, CF

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Recombinant Human PlGF-2 Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Measured in a cell proliferation assay using MDA‑MB‑231 human breast cancer cells. The ED50 for this effect is <10 μg/mL.
Chinese Hamster Ovary cell line, CHO-derived human PlGF-2 protein
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
17.3 kDa (monomer)
28-33 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: PlGF-2

Placenta growth factor (PlGF) is a member of the PDGF/VEGF family of growth factors that share a conserved pattern of eight cysteines (1 ‑ 3). Alternate splicing results in at least three human mature PlGF forms containing 131 (PlGF‑1), 152 (PlGF‑2), and 203 (PlGF‑3) amino acids (aa) respectively (1 ‑ 3). Only PlGF‑2 contains a highly basic heparin‑binding 21 aa insert at the C‑terminus (1). In the mouse, only one PlGF that is the equivalent of human PlGF‑2 has been identified (3). Human PlGF‑2 shares 60%, 56%, 82%, 95% and 95% aa identity with mouse, rat, canine, equine and porcine PlGF‑2. PlGF is mainly found as a variably glycosylated, secreted, 55 ‑ 60 kDa disulfide linked homodimer (4). Mammalian cells expressing PlGF include villous trophoblasts, decidual cells, erythroblasts, keratinocytes and some endothelial cells (1, 5 ‑ 7). Circulating PlGF increases during pregnancy, reaching a peak in mid‑gestation; this increase is attenuated in preeclampsia (8). However, deletion of PlGF in the mouse does not affect development or reproduction. Postnatally, mice lacking PlGF show impaired angiogenesis in response to ischemia (9). PlGF binds and signals through VEGF R1/Flt‑1, but not VEGF  R2/Flk‑1/KDR, while VEGF binds both, but signals only through the angiogenic receptor, VEGF R2. PlGF and VEGF therefore compete for binding to VEGF R1, resulting in a PlGF inhibition of VEGF/VEGF R1 binding coupled to a subsequent promotion of VEGF/VEGF R2‑mediated angiogenesis (1, 5, 9, 10). However, PlGF (especially PlGF‑1) and some forms of VEGF can form dimers that decrease the angiogenic effect of VEGF on VEGF R2 (4, 5). PlGF‑2, like VEGF164/165, shows heparin‑dependent binding of neuropilin (Npn)‑1 and Npn‑2, and can inhibit nerve growth cone collapse (11, 12). PlGF induces monocyte activation, migration, and production of inflammatory cytokines and VEGF. These activities facilitate wound and bone fracture healing, and also contribute to inflammation in active sickle cell disease and atherosclerosis (6, 7, 9, 13 ‑ 16). Circulating PlGF often correlates with tumor stage and aggressiveness, and therapeutic PlGF‑2 antibodies are being investigated for their ability to inhibit tumor growth and angiogenesis (5, 13).

  1. Hauser, S. and H.A. Weich (1993) Growth Factors 9:259.
  2. Maglione, D. et al. (1993) Oncogene 8:925.
  3. DiPalma, T. et al. (1996) Mamm. Genome 7:6.
  4. Eriksson, A. et al. (2002) Cancer Cell 1:99.
  5. Ribatti, D. (2008) Angiogenesis 11:215.
  6. Oura, H. et al. (2003) Blood 101:560.
  7. Roncal, C. et al. (2010) Cardiovasc. Res. 86:29.
  8. Levine, R.J. et al. (2004) N. Engl. J. Med. 350:672.
  9. Carmeliet, P. et al. (2001) Nat. Med. 7:575.
  10. Autiero, M. et al. (2003) Nat. Med. 9:936.
  11. Migdal, M. et al. (1998) J. Biol. Chem. 273:22272.
  12. Cheng, L. et al. (2004) J. Biol. Chem. 279:30654.
  13. Fischer, C. et al. (2008) Nat. Rev. Cancer 8:942.
  14. Perelman, N. et al. (2003) Blood 102:1506.
  15. Cianfarani, F. et al. (2006) Am. J. Pathol. 169:1167.
  16. Maes, C. et al. (2006) J. Clin. Invest. 116:1230.
Long Name
Placenta Growth Factor 2
Entrez Gene IDs
5228 (Human); 18654 (Mouse)
Alternate Names


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Recombinant Human PlGF-2 Protein, CF
By Anonymous on 05/04/2020
Application: Binding assay/Protein-protein interaction
Reason for Rating: % Activity for this molecule was measured and found to be low, ~45% active in solution. This could be due to protein misfolding or errors in the purification process.