Recombinant Human Podocalyxin Protein, CF

Catalog # Availability Size / Price Qty
1658-PD-050
Podocalyxin Inhibits Cell Adhesion to P-Selectin.
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Citations (1)
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Recombinant Human Podocalyxin Protein, CF Summary

Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to inhibit the adhesion of CHO Chinese hamster ovary cells transfected with P-Selectin. The ED50 for this effect is 0.08-0.4 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human Podocalyxin protein
Ser23-Arg427 and a C-terminal Asp and 10-His tag
Accession #
N-terminal Sequence
Analysis
Ser23
Structure / Form
Monomer
Predicted Molecular Mass
43 kDa (monomer)
SDS-PAGE
125-165 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1658-PD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Podocalyxin Inhibits Cell Adhesion to P-Selectin. View Larger

Immobilized Recombinant Human Podocalyxin inhibits the adhesion of CHO Chinese hamster ovary cells transfected with P-Selectin.

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Reconstitution Calculator

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Background: Podocalyxin

Podocalyxin (PODXL), also known as Podocalyxin-like protein 1 (PCLP-1), is a heavily glycosylated transmembrane sialoprotein in the CD34 and Endoglycan family (1). Mature human Podocalyxin consists of a 406 amino acid (aa) extracellular domain (ECD), a 26 aa transmembrane segment, and a 75 aa cytoplasmic domain (2). Within the ECD, human Podocalyxin shares 31% aa sequence identity with mouse and rat Podocalyxin. Alternative splicing of human Podocalyxin generates an additional isoform with a 33 aa deletion in the ECD. Podocalyxin is expressed on glomerular and vascular endothelial cells (2, 3), neurons (4), hematopoietic, mesenchymal, and cardiac stem cells (5-8), and metastatic carcinoma and glioblastoma tumor cells (9-11). A soluble form of Podocalyxin can be released into the urine of women with pre-eclampsia (12). Podocalyxin promotes platelet activation (13), neurite outgrowth, branching, and synaptogenesis (4), homing of hematopoietic progenitor cells to the bone marrow and spleen (6), and engraftment of mesenchymal and cardiac stem cells into ischemic heart and kidney (7, 8). Its interactions with L-Selectin and E-Selectin mediate the tethering of lymphocytes and metastatic tumor cells to the vascular endothelium (3, 9, 10). Podocalyxin can be expressed as a 160-165 kDa molecule or as even larger glycoforms (2, 3, 10, 14). In humans, an approximately 200 kDa glycoform contains the carbohydrate antigen recognized by TRA-1-60 and TRA-1-81 antibodies used for the identification of undifferentiated pluripotent embryonic stem cells (14).

References
  1. Nielsen, J.S. and K.M. McNagny (2009) J. Am. Soc. Nephrol. 20:1669.
  2. Kershaw, D.B. et al. (1997) J. Biol. Chem. 272:15708.
  3. Sassetti, C. et al. (1998) J. Exp. Med. 187:1965.
  4. Vitureira, N. et al. (2010) PLoS ONE 8:e12003.
  5. Zhang, H. et al. (2014) Stem Cells 32:191.
  6. Doyonnas, R. et al. (2005) Blood 105:4170.
  7. Lee, R-H. et al. (2009) Blood 113:816.
  8. Moscoso, I. et al. (2013) J. Tissue Eng. Regen. Med. Epub. PMID 23897803.
  9. Dallas, M.R. et al. (2012) Am. J. Physiol. Cell Physiol. 303:C616.
  10. Thomas, S.N. et al. (2009) Am. J. Cell Physiol. 296:C505.
  11. Binder, Z.A. et al. (2013) PLoS ONE 8:e75945.
  12. Wang, Y. et al. (2012) Am. J. Physiol. Renal Physiol. 302:F1084.
  13. Alonso-Martin, S. et al. (2010) Thromb. Res. 125:e300.
  14. Schopperle, W.M. and W.C. DeWolf (2007) Stem Cells 25:723.
Entrez Gene IDs
5420 (Human); 27205 (Mouse)
Alternate Names
GCTM; Gp200MGC138240; PCLP; PCLP1; PCLP-1; PCLPGCTM-2 antigen; PCpodocalyxin; POD XL; Podocalyxin; Podocalyxin-like protein 1; podocalyxin-like; PODXL

Citation for Recombinant Human Podocalyxin Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucins
    Authors: SA Malaker, K Pedram, MJ Ferracane, BA Bensing, V Krishnan, C Pett, J Yu, EC Woods, JR Kramer, U Westerlind, O Dorigo, CR Bertozzi
    Proc. Natl. Acad. Sci. U.S.A., 2019;0(0):.
    Species: Bacteria - E. Coli
    Sample Types: Recombinant Protein
    Applications: Bioassay

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