Immobilized Recombinant Human Podocalyxin inhibits the adhesion of CHO Chinese hamster ovary cells transfected with P-Selectin.
Podocalyxin (PODXL), also known as Podocalyxin-like protein 1 (PCLP-1), is a heavily glycosylated transmembrane sialoprotein in the CD34 and Endoglycan family (1). Mature human Podocalyxin consists of a 406 amino acid (aa) extracellular domain (ECD), a 26 aa transmembrane segment, and a 75 aa cytoplasmic domain (2). Within the ECD, human Podocalyxin shares 31% aa sequence identity with mouse and rat Podocalyxin. Alternative splicing of human Podocalyxin generates an additional isoform with a 33 aa deletion in the ECD. Podocalyxin is expressed on glomerular and vascular endothelial cells (2, 3), neurons (4), hematopoietic, mesenchymal, and cardiac stem cells (5-8), and metastatic carcinoma and glioblastoma tumor cells (9-11). A soluble form of Podocalyxin can be released into the urine of women with pre-eclampsia (12). Podocalyxin promotes platelet activation (13), neurite outgrowth, branching, and synaptogenesis (4), homing of hematopoietic progenitor cells to the bone marrow and spleen (6), and engraftment of mesenchymal and cardiac stem cells into ischemic heart and kidney (7, 8). Its interactions with L-Selectin and E-Selectin mediate the tethering of lymphocytes and metastatic tumor cells to the vascular endothelium (3, 9, 10). Podocalyxin can be expressed as a 160-165 kDa molecule or as even larger glycoforms (2, 3, 10, 14). In humans, an approximately 200 kDa glycoform contains the carbohydrate antigen recognized by TRA-1-60 and TRA-1-81 antibodies used for the identification of undifferentiated pluripotent embryonic stem cells (14).
Nielsen, J.S. and K.M. McNagny (2009) J. Am. Soc. Nephrol. 20:1669.
Kershaw, D.B. et al. (1997) J. Biol. Chem. 272:15708.
Sassetti, C. et al. (1998) J. Exp. Med. 187:1965.
Vitureira, N. et al. (2010) PLoS ONE 8:e12003.
Zhang, H. et al. (2014) Stem Cells 32:191.
Doyonnas, R. et al. (2005) Blood 105:4170.
Lee, R-H. et al. (2009) Blood 113:816.
Moscoso, I. et al. (2013) J. Tissue Eng. Regen. Med. Epub. PMID 23897803.
Dallas, M.R. et al. (2012) Am. J. Physiol. Cell Physiol. 303:C616.
Thomas, S.N. et al. (2009) Am. J. Cell Physiol. 296:C505.
Binder, Z.A. et al. (2013) PLoS ONE 8:e75945.
Wang, Y. et al. (2012) Am. J. Physiol. Renal Physiol. 302:F1084.
Alonso-Martin, S. et al. (2010) Thromb. Res. 125:e300.
Schopperle, W.M. and W.C. DeWolf (2007) Stem Cells 25:723.
Entrez Gene IDs:
5420 (Human); 27205 (Mouse)
GCTM; Gp200MGC138240; PCLP; PCLP1; PCLP-1; PCLPGCTM-2 antigen; PCpodocalyxin; POD XL; Podocalyxin-like protein 1; podocalyxin-like; PODXL
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.