Recombinant Human Podocalyxin Protein, CF

  
  • Purity
    >95%, by SDS-PAGE with silver staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by the ability of the immobilized protein to inhibit the adhesion of CHO Chinese hamster ovary cells transfected with P-Selectin. The ED50 for this effect is 0.08-0.4 μg/mL.
  • Source
    Mouse myeloma cell line, NS0-derived Ser23-Arg427 and a C-terminal Asp and 10-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Ser23
  • Structure / Form
    Monomer
  • Predicted Molecular Mass
    43 kDa (monomer)
  • SDS-PAGE
    125-165 kDa, reducing conditions
1658-PD
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Podocalyxin Inhibits Cell Adhesion to P-Selectin.
Immobilized Recombinant Human Podocalyxin inhibits the adhesion of CHO Chinese hamster ovary cells transfected with P-Selectin.
Background: Podocalyxin
Podocalyxin (PODXL), also known as Podocalyxin-like protein 1 (PCLP-1), is a heavily glycosylated transmembrane sialoprotein in the CD34 and Endoglycan family (1). Mature human Podocalyxin consists of a 406 amino acid (aa) extracellular domain (ECD), a 26 aa transmembrane segment, and a 75 aa cytoplasmic domain (2). Within the ECD, human Podocalyxin shares 31% aa sequence identity with mouse and rat Podocalyxin. Alternative splicing of human Podocalyxin generates an additional isoform with a 33 aa deletion in the ECD. Podocalyxin is expressed on glomerular and vascular endothelial cells (2, 3), neurons (4), hematopoietic, mesenchymal, and cardiac stem cells (5-8), and metastatic carcinoma and glioblastoma tumor cells (9-11). A soluble form of Podocalyxin can be released into the urine of women with pre-eclampsia (12). Podocalyxin promotes platelet activation (13), neurite outgrowth, branching, and synaptogenesis (4), homing of hematopoietic progenitor cells to the bone marrow and spleen (6), and engraftment of mesenchymal and cardiac stem cells into ischemic heart and kidney (7, 8). Its interactions with L-Selectin and E-Selectin mediate the tethering of lymphocytes and metastatic tumor cells to the vascular endothelium (3, 9, 10). Podocalyxin can be expressed as a 160-165 kDa molecule or as even larger glycoforms (2, 3, 10, 14). In humans, an approximately 200 kDa glycoform contains the carbohydrate antigen recognized by TRA-1-60 and TRA-1-81 antibodies used for the identification of undifferentiated pluripotent embryonic stem cells (14).
  • References:
    1. Nielsen, J.S. and K.M. McNagny (2009) J. Am. Soc. Nephrol. 20:1669.
    2. Kershaw, D.B. et al. (1997) J. Biol. Chem. 272:15708.
    3. Sassetti, C. et al. (1998) J. Exp. Med. 187:1965.
    4. Vitureira, N. et al. (2010) PLoS ONE 8:e12003.
    5. Zhang, H. et al. (2014) Stem Cells 32:191.
    6. Doyonnas, R. et al. (2005) Blood 105:4170.
    7. Lee, R-H. et al. (2009) Blood 113:816.
    8. Moscoso, I. et al. (2013) J. Tissue Eng. Regen. Med. Epub. PMID 23897803.
    9. Dallas, M.R. et al. (2012) Am. J. Physiol. Cell Physiol. 303:C616.
    10. Thomas, S.N. et al. (2009) Am. J. Cell Physiol. 296:C505.
    11. Binder, Z.A. et al. (2013) PLoS ONE 8:e75945.
    12. Wang, Y. et al. (2012) Am. J. Physiol. Renal Physiol. 302:F1084.
    13. Alonso-Martin, S. et al. (2010) Thromb. Res. 125:e300.
    14. Schopperle, W.M. and W.C. DeWolf (2007) Stem Cells 25:723.
  • Entrez Gene IDs:
    5420 (Human); 27205 (Mouse)
  • Alternate Names:
    GCTM; Gp200MGC138240; PCLP; PCLP1; PCLP-1; PCLPGCTM-2 antigen; PCpodocalyxin; POD XL; Podocalyxin; Podocalyxin-like protein 1; podocalyxin-like; PODXL
Related Research Areas
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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