Recombinant Human Protocadherin-17 Protein, CF Summary
Leu18-Ser705, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 400 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human Protocadherin 17 (PCDH17; also called Protocadherin‑68) is an approximately 1159 amino acid (aa) glycoprotein belonging to the δ2 subgroup of protocadherins, type I transmembrane proteins that exhibit calcium‑dependent but relatively weak homophilic adhesion (1‑3). Like other δ2 protocadherins, PCDH17 cDNA encodes a signal sequence (aa 1‑17), six cadherin domains of about 100 aa each (aa 18‑695), a transmembrane sequence and a cytoplasmic domain (1). It contains an extracellular RGD cell attachment motif that may mediate integrin adhesion (3). Typical of protocadherins, an alternately spliced 889 aa form of human PCDH17 diverges at aa 876 within the cytoplasmic domain and lacks two conserved cytoplasmic motifs (1, 2). Within the extracellular domain, human PCDH17 shares 98% aa sequence identity with mouse and bovine, 99% with rat and equine, and 97% with porcine PCDH17. PCDH17 is constitutively expressed in the central nervous system (3). It is one of several PCDH molecules that shows region specificity in the basal ganglia, forming gradients (3). Early in postnatal life, it is preferentially expressed in motor and auditory cortices and is correlated with thalamo‑cortical and intrahippocampal circuits (3‑5). Its expression is increased in the Broadman area of the brain in subjects with schizophrenia (3). PCDH17 is frequently silenced in squamous cell carcinomas such as those in the esophagus and larynx, especially if they are poorly differentiated (3, 6, 7). Ectopic PCDH10 expression reduces tumor cell proliferation and migration (6).
- Redies, C. et al. (2005) Cell. Mol. Life Sci. 62:2840.
- Vanhalst, K. et al. (2005) Cell. Mol. Life Sci. 62:1247.
- Kim, S. Y. et al. (2011) Cell Adh. Migr. 5:97.
- Kim, S. Y. et al. (2007) Neuroscience 147:996.
- Kim, S. Y. et al. (2010) Neuroscience 170:189.
- Haruki, S. et al. (2010) Carcinogenesis 31:1027.
- Giefing, M. et al. (2011) Genes Chromosomes Cancer 50:154.
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