Recombinant Human PSG1 Protein, CF

Catalog # Availability Size / Price Qty
9334-P1-050
Recombinant Human PSG1 Protein Binding Activity
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Recombinant Human PSG1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human SLITRK5 (Catalog # 2587-SK) is coated at 500 ng/mL, Recombinant Human PSG1 (Catalog # 9334-P1) binds with an ED50 = 50-300 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human PSG1 protein
Gln35-Pro419, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Gln35 & Val36
Predicted Molecular Mass
44 kDa
SDS-PAGE
64-77 kDa, reducing conditions

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9334-P1

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9334-P1

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 2 weeks, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Human PSG1 Protein Binding Activity View Larger

When Recombinant Human SLITRK5 (Catalog # 2587-SK) is coated at 500 ng/mL, Recombinant Human PSG1 (Catalog # 9334-P1) binds with an ED50 = 50-300 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PSG1

Pregnancy-specific beta-1-glycoprotein 1 (PSG1), also called SP1, PSG1 or B1G1 and designated CD66f, is a secreted glycoprotein of the human PSG family within the CEA (carcinoembryonic antigen) superfamily (1-3). PSG1 shares 84-91% amino acid (aa) sequence identity with the most similar family members, PSG3, 4, 6, 7, and 8. Mature PSG1 contains a V-type Ig-like domain that is important for adhesion (N1), and three C2-type Ig-like domains (A1, A2 and B2). Three potential isoforms vary at the extreme C-terminus. Although rodents express pregnancy-specific glycoproteins, identity with human PSG proteins is low and limited to the N1 domain (3). PSG1 is one of the most abundant fetal proteins found in maternal blood during pregnancy. It is secreted by syncytiotrophoblasts and exerts proangiogenic effect by inducing VEGF-A secretion and formation of tubes in endothelial cells (4). PSG1 also serves an immunomodulatory function through
up-regulation of TGF-beta in macrophages, monocytes, and trophoblasts (5). In addition PSG1 has been shown to induce secretion of anti-inflammatory cytokines
IL-10 and IL-6 in human monocytes (6). PSG1 binds Integrin alpha IIb beta 3 and inhibits platelet-fibrinogen interactions (7). Recent studies also suggest PSG1's involvement in cancer biology and correlation with chemotherapy resistance in breast cancer (8).

References
  1. Watanabe, S. and J.Y. Chou (1988) J. Biol. Chem. 263:2049.
  2. Leslie, K.K. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5822.
  3. McLellan, A.S. et al. (2005) BMC Evol. Biol. 5:39.
  4. Lisboa, F. et al. (2011) J Biol Chem. 286:7577.
  5. Ha, C. et al. (2010) Biol Rep. 83:27.
  6. Snyder, S. et al. (2001) Am J Reprod Immunol. 45:205.
  7. Shanley D. et al. (2013) PLoS One. 8:e57491.
  8. He, D. et al. (2016) Clin Sci (Lond). 130:2267.
Long Name
Pregnancy-specific beta-1-glycoprotein 1
Entrez Gene IDs
5669 (Human)
Alternate Names
B1G1; B1G1CD66 antigen-like family member F; CD66f antigen; CD66f; DHFRP2; Fetal liver non-specific cross-reactive antigen 1/2; FLJ90598; FLJ90654; FL-NCA-1/2; PBG1; pregnancy specific beta-1-glycoprotein 1; pregnancy-specific B-1 glycoprotein; Pregnancy-specific beta-1 glycoprotein C/D; pregnancy-specific beta-1-glycoprotein 1; Pregnancy-specific glycoprotein 1; PS-beta-C/D; PS-beta-G-1; PSbG1; PSBG-1; PSBG1SP1; PSG1; PSG-1; PSG95; PSGGA; PSGGAPSG95; PSGIIA

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