>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Human SLITRK5
is coated at 500 ng/mL, Recombinant Human PSG1
(Catalog # 9334-P1) binds with an ED50 = 50-300 ng/mL.
Mouse myeloma cell line, NS0-derived Gln35-Pro419, with a C-terminal 6-His tag
When Recombinant Human SLITRK5 (Catalog # 2587-SK) is coated at 500 ng/mL,Recombinant Human PSG1(Catalog # 9334-P1)binds withan ED50 = 50-300ng/mL.
Pregnancy-specific beta-1-glycoprotein 1
(PSG1), also called SP1, PSG1 or B1G1 and designated CD66f, is a secreted
glycoprotein of the human PSG family within the CEA (carcinoembryonic antigen)
superfamily (1-3). PSG1 shares 84-91% amino acid (aa) sequence identity with
the most similar family members, PSG3, 4, 6, 7, and 8. Mature PSG1
contains a V-type Ig-like domain that is important for adhesion (N1), and three
C2-type Ig-like domains (A1, A2 and B2). Three potential isoforms vary at the
extreme C-terminus. Although rodents express pregnancy-specific
glycoproteins, identity with human PSG proteins is low and limited to the N1 domain (3). PSG1
is one of the most abundant fetal proteins found in maternal blood during
pregnancy. It is secreted by syncytiotrophoblasts and exerts proangiogenic
effect by inducing VEGF-A secretion and formation of tubes in endothelial cells
(4). PSG1 also serves an immunomodulatory function through up-regulation of
TGF-beta in macrophages, monocytes, and trophoblasts (5). In addition PSG1 has been shown to induce
secretion of anti-inflammatory cytokines IL-10 and IL-6 in human monocytes (6). PSG1 binds Integrin alpha IIb beta 3 and inhibits
platelet-fibrinogen interactions (7).
Recent studies also suggest PSG1's involvement in cancer biology and
correlation with chemotherapy resistance in breast cancer (8).
Watanabe, S. and J.Y. Chou (1988) J. Biol. Chem. 263:2049.
Leslie, K.K. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5822.
McLellan, A.S. et al. (2005) BMC Evol. Biol. 5:39.
Lisboa, F. et al. (2011) J Biol Chem. 286:7577.
Ha, C. et al. (2010) Biol Rep. 83:27.
Snyder, S. et al. (2001) Am J Reprod Immunol. 45:205.
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