Recombinant Human PTH Protein, CF

Catalog # Availability Size / Price Qty
7665-PT-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human PTH Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce cAMP accumulation in MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 10-60 ng/mL.
Source
E. coli-derived human PTH protein
Ser32-Gln115
Accession #
N-terminal Sequence
Analysis
Ser32
Structure / Form
Monomer
Predicted Molecular Mass
9.5 kDa
SDS-PAGE
9 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7665-PT

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

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Background: PTH

PTH (parathyroid hormone) is a critical hormone in the regulation of Ca++ homeostasis (1). The human PTH cDNA encodes 115 amino acids (aa) including a 25 aa signal sequence, a 6 aa propeptide, and an 84 aa mature hormone. Mature human PTH shares 70%, 73%, 88%, 87%, 86%, 86% and 85% aa identity with mouse, rat, canine, equine, bovine, porcine and feline PTH, respectively. Multiple N‑terminal peptides and C-terminal peptides derived from PTH occur naturally in the circulation (1). PTH aa 32-66, called PTH (1-34) since it represents the first 34 aa of the mature hormone, reproduces all the activity of the full length mature hormone and has been used therapeutically for treatment of osteoporosis (1-3). C-terminal peptides mainly oppose the activities of PTH (1-34) and are increased in renal failure (1-3). PTH expression is mainly restricted to the parathyroid gland, with minor amounts in the thymus (4). PTH secretion is enhanced by low Ca++ concentrations and inhibited by FGF-23 (1, 5). In normal human plasma, PTH correlates negatively with active Vitamin D and positively with ionized calcium (6). Human and other mammalian PTH will bind and stimulate human or rat PTH1R, activating adenylate cyclase and increasing cAMP production (2, 7). PTH promotes secretion of TRANCE/RANKL and periostin through PTH1R binding on osteoblasts and/or bone marrow stromal cells (8-10). TRANCE/RANKL induces differentiation of osteoclasts, which in turn promote release of Ca++ from bone (1, 8, 9). PTH1R on osteocytes, however, allows PTH to promote bone formation and IGF-1 production (11, 12). In renal epithelium, PTH promotes conversion of Vitamin D to its active form, lowers Ca++ excretion and increases phosphate excretion (1, 2, 9). PTH also increases hematopoietic stem cell proliferation and mobilization and induces arterial vasodilation by regulating Ca++ influx in PTH1R-expressing arterial smooth muscle (8, 13).

References
  1. Potts, J.T. (2005) J. Endocrinol. 187:311.
  2. Usatii, M. et al. (2007) Kidney Int. 72:1330.
  3. Scillitani, A. et al. (2011) J. Clin. Invest. 34:23.
  4. Liu, Z. et al. (2010) PLoS Genet. 6:e1001251.
  5. Ben-Dov, I.Z. et al. (2007) J. Clin. Invest. 117:4003.
  6. Carnevale, V. et al. (2010) Bone 47:626.
  7. Schipani, E. et al. (1993) Endocrinology 132:2157.
  8. Jacome-Galarza, C.E. et al. (2011) J. Bone Miner. Res. 26:1207.
  9. Lupp, A. et al. (2010) Eur. J. Endocrinol. 162:979.
  10. Fortunati, D. et al. (2010) Matrix Biol. 29:594.
  11. O’Brien, C.A. et al. (2008) PLoS ONE 3:e2942.
  12. Lombardi, G. et al. (2010) J. Endocrinol. Invest. 33:22.
  13. Zaruba, M.M. et al. (2008) Cardiovasc. Res. 77:722.
Long Name
Parathyroid Hormone
Entrez Gene IDs
5741 (Human)
Alternate Names
Parathormone; Parathyrin; parathyroid hormone 1; parathyroid hormone; PTH; PTH1

Citation for Recombinant Human PTH Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The transcription factor protein Sox11 enhances early osteoblast differentiation by facilitating proliferation and the survival of mesenchymal and osteoblast progenitors.
    Authors: Gadi, Jogeswar, Jung, Seung-Hy, Lee, Min-Jung, Jami, Ajita, Ruthala, Kalyani, Kim, Kyoung-M, Cho, Nam-Hoon, Jung, Han-Sung, Kim, Cheol-He, Lim, Sung-Kil
    J Biol Chem, 2013;288(35):25400-13.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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