Recombinant Human PTH Protein, CF Summary
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 250 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
PTH (parathyroid hormone) is a critical hormone in the regulation of Ca++ homeostasis (1). The human PTH cDNA encodes 115 amino acids (aa) including a 25 aa signal sequence, a 6 aa propeptide, and an 84 aa mature hormone. Mature human PTH shares 70%, 73%, 88%, 87%, 86%, 86% and 85% aa identity with mouse, rat, canine, equine, bovine, porcine and feline PTH, respectively. Multiple N‑terminal peptides and C-terminal peptides derived from PTH occur naturally in the circulation (1). PTH aa 32-66, called PTH (1-34) since it represents the first 34 aa of the mature hormone, reproduces all the activity of the full length mature hormone and has been used therapeutically for treatment of osteoporosis (1-3). C-terminal peptides mainly oppose the activities of PTH (1-34) and are increased in renal failure (1-3). PTH expression is mainly restricted to the parathyroid gland, with minor amounts in the thymus (4). PTH secretion is enhanced by low Ca++ concentrations and inhibited by FGF-23 (1, 5). In normal human plasma, PTH correlates negatively with active Vitamin D and positively with ionized calcium (6). Human and other mammalian PTH will bind and stimulate human or rat PTH1R, activating adenylate cyclase and increasing cAMP production (2, 7). PTH promotes secretion of TRANCE/RANKL and periostin through PTH1R binding on osteoblasts and/or bone marrow stromal cells (8-10). TRANCE/RANKL induces differentiation of osteoclasts, which in turn promote release of Ca++ from bone (1, 8, 9). PTH1R on osteocytes, however, allows PTH to promote bone formation and IGF-1 production (11, 12). In renal epithelium, PTH promotes conversion of Vitamin D to its active form, lowers Ca++ excretion and increases phosphate excretion (1, 2, 9). PTH also increases hematopoietic stem cell proliferation and mobilization and induces arterial vasodilation by regulating Ca++ influx in PTH1R-expressing arterial smooth muscle (8, 13).
- Potts, J.T. (2005) J. Endocrinol. 187:311.
- Usatii, M. et al. (2007) Kidney Int. 72:1330.
- Scillitani, A. et al. (2011) J. Clin. Invest. 34:23.
- Liu, Z. et al. (2010) PLoS Genet. 6:e1001251.
- Ben-Dov, I.Z. et al. (2007) J. Clin. Invest. 117:4003.
- Carnevale, V. et al. (2010) Bone 47:626.
- Schipani, E. et al. (1993) Endocrinology 132:2157.
- Jacome-Galarza, C.E. et al. (2011) J. Bone Miner. Res. 26:1207.
- Lupp, A. et al. (2010) Eur. J. Endocrinol. 162:979.
- Fortunati, D. et al. (2010) Matrix Biol. 29:594.
- O’Brien, C.A. et al. (2008) PLoS ONE 3:e2942.
- Lombardi, G. et al. (2010) J. Endocrinol. Invest. 33:22.
- Zaruba, M.M. et al. (2008) Cardiovasc. Res. 77:722.
Citation for Recombinant Human PTH Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
The transcription factor protein Sox11 enhances early osteoblast differentiation by facilitating proliferation and the survival of mesenchymal and osteoblast progenitors.
Authors: Gadi, Jogeswar, Jung, Seung-Hy, Lee, Min-Jung, Jami, Ajita, Ruthala, Kalyani, Kim, Kyoung-M, Cho, Nam-Hoon, Jung, Han-Sung, Kim, Cheol-He, Lim, Sung-Kil
J Biol Chem, 2013;288(35):25400-13.
Sample Types: Whole Cells
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