Recombinant Human PTPR sigma Protein, CF

Catalog # Availability Size / Price Qty
3430-PR-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human PTPR sigma Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human PTPR sigma /PTPRS  is coated at 2 μg/mL, Recombinant Human TrkC Fc Chimera (Catalog # 373‑TC) binds with an apparent Kd <1 nM.
Source
Mouse myeloma cell line, NS0-derived human PTP sigma/PTPRS protein
Glu30-Gly1260, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Glu30 & Ser1173
Predicted Molecular Mass
135.8 kDa
SDS-PAGE
120-145 kDa, reducing conditions

Product Datasheets

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3430-PR

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3430-PR

Formulation Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

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Background: PTP sigma/PTPRS

Phosphatase Receptor-type sigma (PTPR sigma ), also known as PTP sigma, is a 200 kDa type I transmembrane glycoprotein that is a member of the class 2A (LAR) subfamily of the protein tyrosine phosphatase (PTP) family (1). Human PTPR sigma cDNA encodes 1948 amino acids (aa) including a 29 aa signal sequence, a 1253 aa extracellular domain (ECD) with three C2-type Ig domains and eight Fibronectin (Fn) type III domains, a transmembrane domain, and a cytoplasmic region with two PTP domains (1, 2). Multiple differentially expressed splice variants lack sections of the Ig and/or Fn domains and/or cytoplasmic juxtamembrane sequence (1, 2). PKC activation in the cell promotes endoprotease cleavage of PTPR sigma, releasing the 140-160 kDa ECD (3). The human PTPR sigma ECD shares 93% aa sequence identity with the nearest isoform of mouse and rat PTPR sigma, 96% with bovine PTPR sigma, and 97% with porcine PTPR sigma. Activity of PTPR sigma can be inhibited by bisphosphonates (2). PTPR sigma is a receptor for several proteins, including neural chondroitin sulfate proteoglycans which inhibit nerve regeneration (4, 5). It also binds and dephosphorylates targets such as N-Cadherin, E-Cadherin, EGF R and Ret (6-9). Through these interactions PTPR sigma affects pituitary and neuroendocrine development, and axon growth and targeting (4-6). Mice deleted for PTPR sigma show pituitary, pancreatic, and gut abnormalities, mainly due to growth hormone insufficiency (9-12). They also show enhanced regeneration, but faulty targeting, of injured peripheral nerves (4-6, 13). Head and neck squamous cell cancers frequently underexpress PTPR sigma (14). Since PTPR sigma inhibits EGF R signaling pathways, its underexpression can enhance tumor cell growth (14).

References
  1. Pulido, R. et al. (1995) Proc. Natl. Acad. Sci. USA 92:11686.
  2. Endo, N. et al. (1996) J. Bone Miner. Res. 11:535.
  3. Aicher, B. et al. (1997) J. Cell Biol. 138:681.
  4. Shen, Y. et al. (2009) Science 326:592.
  5. Duan, Y. and R.J. Giger (2010) Sci. Signal. 3:pe6.
  6. Siu, R. et al. (2007) Mol. Cell. Biol. 27:208.
  7. Vijayvargia, R. et al. (2004) Biochem. Biophys. Res. Commun. 325:344.
  8. Uetani, N. et al. (2009) J. Clin. Invest. 119:924.
  9. Muise, A.M. et al. (2007) Curr. Biol. 17:1212.
  10. Elchebly, M. et al. (1999) Nat. Genet. 21:330.
  11. Batt, J. et al. (2002) Mol. Endocrinol. 16:155.
  12. Chagnon, M.J. et al. (2006) Can. J. Physiol. Pharmacol. 84:755.
  13. McLean, J. et al. (2002) J. Neurosci. 22:5481.
  14. Morris, L.G. et al. (2011) Proc. Natl. Acad. Sci. USA 108:19024.
Long Name
Protein Tyrosine Phosphatase Receptor-type Sigma
Entrez Gene IDs
5802 (Human); 19280 (Mouse)
Alternate Names
EC 3.1.3.48; protein tyrosine phosphatase PTPsigma; protein tyrosine phosphatase, receptor type, S; protein tyrosine phosphatase, receptor type, sigma; PTP sigma; PTPR sigma; PTPRS; PTPSIGMA; receptor-type tyrosine-protein phosphatase S; Receptor-type tyrosine-protein phosphatase sigma; R-PTP-S; R-PTP-sigma

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