Recombinant Human PTPRD Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
9995-PR-050
Recombinant Human PTPRD Fc Chimera Protein, CF Binding Activity
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Recombinant Human PTPRD Fc Chimera Protein, CF Summary

Purity
>80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human PTPRD Fc Chimer is immobilized at 1 μg/mL, 100 μL/well, Recombinant Human IL-1 RAPL1/IL-1 R8 Fc Chimera (Catalog # 9949-ML) binds with an ED50 of 1.5-9 ng/mL. 
Source
Human embryonic kidney cell, HEK293-derived human PTPRD protein
Human PTPRD
(Glu21-Ser1174)
Accession # P23468
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Glu21
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
154 kDa
SDS-PAGE
137-151 kDa, reducing conditions

Product Datasheets

9995-PR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Image

Binding Activity Recombinant Human PTPRD Fc Chimera Protein, CF Binding Activity View Larger

When Recombinant Human PTPRD Fc Chimera is immobilizedat 1 μg/mL, 100 μL/well, Recombinant Human IL-1 RAPL1/IL-1 R8 FcChimera (Catalog # 9949-ML) binds with an ED50 of 1.5-9 ng/mL.

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Background: PTPRD

Human protein-tyrosine phosphatase delta (PTPRD) is a type I membrane protein. It contains a 1245 amino acid (aa) long extracellular domain (ECD), a 25 amino acid long transmembrane domain, and a 622 amino acid cytoplasmic domain. A cleavage during post-translational modification separates the extracellular domain from the transmembrane segment through a process called "ectodomain shedding" (1). The extracellular regions are comprised of three Ig-like C2 domains followed immediately by eight fibronectin type-III like domains (1). The human PTPRD extracellular domain shares 98% and 62% aa identity with mouse and rat PTPRD, respectively. Protein-tyrosine phosphatases (PTPs) constitute a structurally diverse family of tightly regulated enzymes with important regulatory roles (1-6). PTPRD is a member of the PTPs and is detected in brain and other tissues including colon and breast (1). It has been demonstrated that phosphorylated STAT3 (p-STAT3) is a direct substrate of PTPRD and that cancer-specific mutations in PTPRD abrogate its ability to dephosphorylate STAT3 (5). PTPRD interacts with NGL-3 (Netrin-G ligand-3) via its first two FNIII repeats to promote synapse formation (3). PTPRD can also bind to IL1RAPL1 and its paralog IL1RAPL2; the IL1RAPL1/PTPRD complex recruits RhoGAP2 at excitatory synapses to induce dendritic spine formation (4). Recent studies have indicated SALM5 forms heterotetramer with PTPRD to induce synaptic differentiation (6).

References
  1. Pulido, R. et al. (1995) Proc. Natl. Acad. Sci. USA 92:11686.
  2. Östman, A. et al. (2006) Nature Reviews Cancer 6:307.
  3. Kwon, SK. et al. (2010) J. Biol. Chem. 285:13966.
  4. Valnegri, P. et al. (2011) Hum. Mol. Genet. 20:4797.
  5. Ortiz, B. et al. (2014) Proc. Natl. Acad. Sci. USA. 111:8149.
  6. Lin, Z. et al. (2018) Nat, Commun. 9:268.
Long Name
Protein Tyrosine Phosphatase Receptor-type delta
Entrez Gene IDs
5789 (Human); 19266 (Mouse); 313278 (Rat)
Alternate Names
HPTPDELTA; HPTPMGC119752; MGC119750; MGC119751; protein tyrosine phosphatase, receptor type, D; Protein-tyrosine phosphatase delta; PTP delta; PTPD; PTPdelta; PTP-delta; PTPDMGC119753; PTPRD; receptor type, delta polypeptide; receptor-type tyrosine-protein phosphatase delta; R-PTP-Delta; RPTP-Delta

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