Recombinant Human R-Spondin 2 Protein

Carrier Free

Catalog # Availability Size / Price Qty
3266-RS-025/CF

With Carrier

Catalog # Availability Size / Price Qty
3266-RS-025
Recombinant Human R-Spondin 2 Protein Bioactivity
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Product Details
Citations (15)
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Recombinant Human R-Spondin 2 Protein Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED50 is 0.7-2.8 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt-3a.
Source
Mouse myeloma cell line, NS0-derived human R-Spondin 2 protein
Gln22-Gly205, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
No results obtained: Gln22 predicted
Predicted Molecular Mass
22 kDa
SDS-PAGE
25-29 kDa, reducing conditions

Product Datasheets

3266-RS (with carrier)

3266-RS/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3266-RS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

3266-RS/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Recombinant Human R-Spondin 2 Protein Bioactivity View Larger

Recombinant R-Spondin 2 (Catalog # 3266-RS) induces activation of beta -catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is 0.7-2.8 ng/mL in the presence of 5 ng/mL of Recombinant Mouse Wnt-3a (Catalog # 1324-WN).

SDS-PAGE Recombinant Human R-Spondin 2 Protein SDS-PAGE View Larger

1 µg/lane of Recombinant Human R-Spondin 2 was resolved with SDS-PAGE and visualized by silver staining under reducing (R) conditions, showing a single band at 28 kDa.

Reconstitution Calculator

Reconstitution Calculator

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Background: R-Spondin 2

Roof plate-specific Spondin 2 isoform 1 (R‑Spondin 2, RSPO2), also known as cysteine‑rich and single thrombospondin domain containing protein 2 (Cristin 2), is a 33 kDa secreted protein that belongs to the R-Spondin family (1‑3). The four R‑Spondins regulate Wnt/ beta -catenin signaling and overlap in expression and function (1‑3). Like other R‑Spondins, RSPO2 contains two adjacent cysteine‑rich furin-like domains (aa 90‑134) followed by a thrombospondin (TSP-1) motif (aa 144‑204) and a C-terminal region rich in basic residues (aa 207‑243). The basic region binds heparin and mediates cell surface retention and extracellular matrix attachment while the furin‑like domains are required for Wnt/ beta -catenin signaling (1, 3, 4). RSPO2 contains one potential N‑glycosylation site. Mature human RSPO2 shares 97‑98% aa identity with mouse, rat, equine, canine and bovine RSPO2 and ~40% aa identity with RSPO1, RSPO3 and RSPO4. Of the three reported splice isoforms of human R‑Spondin 2, isoform 2 lacks residues 1 ‑ 67 of isoform 1, while isoform 3 has a glycine substitution for residues 32‑95 of isoform 1 (5). Human RSPO2 is expressed in organs of endodermal origin in adults, including intestine and lung, and is down‑regulated in tumors of these tissues (1). In the embryonic mouse, RSPO2 expression is concentrated in the apical epidermal ridge, hippocampus, and developing muscle, teeth and bones (1, 6). Deletion of RSPO2 results in down‑regulation of Wnt activity in these areas, malformations of the facial skeleton and limbs, and respiratory failure at birth (7‑9). RSPO2 is an extracellular potentiator of Wnt/ beta -catenin signaling (3, 4). It functions at least in part by binding LRP‑6, stimulating its long-term phosphorylation and down‑regulating its internalization (3, 4). RSPO proteins, especially RSPO2 and RSPO3, also antagonize DKK1 activity by interfering with DKK1‑mediated LRP‑6 and Kremen association (10).

References
  1. Kazanskaya, O. et al. (2004) Dev. Cell 7:525.
  2. Kim, K.-A. et al. (2006) Cell Cycle 5:23.
  3. Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
  4. Li, S-J. et al. (2009) Cell Signal. 21:916.
  5. Swiss-Prot Accession # Q6UXX9.
  6. Nam, J.-S. et al. (2007) Gene Expr. Patterns 7:306.
  7. Yamada, W. et al. (2009) Biochem. Biophys. Res. Commun. 381:453.
  8. Jin, Y.-R. et al. (2011) Dev. Biol. 352:1.
  9. Nam, J.-S. et al. (2007) Dev. Biol. 311:124.
  10. Kim, K.-A. et al. (2007) Mol. Biol. Cell 19:2588.
Long Name
Roof Plate-specific Spondin 2
Entrez Gene IDs
340419 (Human); 239405 (Mouse)
Alternate Names
Cristin 2; CRISTIN2; hRspo2; MGC35555; MGC43342; Roof plate-specific spondin-2; RSPO2; R-spondin 2 homolog (Xenopus laevis); RSpondin 2; R-Spondin 2; R-spondin-2

Citations for Recombinant Human R-Spondin 2 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Fallopian Tube Basal Stem Cells Reproducing the Epithelial Sheets In Vitro-Stem Cell of Fallopian Epithelium
    Authors: M Zhu, T Iwano, S Takeda
    Biomolecules, 2020;10(9):.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Targeting the Wnt signaling pathway through R-spondin 3 identifies an anti-fibrosis treatment strategy for multiple organs
    Authors: M Zhang, M Haughey, NY Wang, K Blease, AM Kapoun, S Couto, I Belka, T Hoey, M Groza, J Hartke, B Bennett, J Cain, A Gurney, B Benish, P Castiglion, C Drew, J Lachowicz, L Carayannop, SD Nathan, J Distler, DA Brenner, K Hariharan, H Cho, W Xie
    PLoS ONE, 2020;15(3):e0229445.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC Control
  3. δN-Tp63 Mediates Wnt/ β-Catenin-Induced Inhibition of Differentiation in Basal Stem Cells of Mucociliary Epithelia
    Authors: M Haas, JL Gómez Vázq, DI Sun, HT Tran, M Brislinger, A Tasca, O Shomroni, K Vleminckx, P Walentek
    Cell Rep, 2019;28(13):3338-3352.e6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Wnt Signalling Drives Context-Dependent Differentiation or Proliferation in Neuroblastoma
    Authors: M Szemes, A Greenhough, Z Melegh, S Malik, A Yuksel, D Catchpoole, K Gallacher, M Kollareddy, JH Park, K Malik
    Neoplasia, 2018;20(4):335-350.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. R-spondins can potentiate WNT signaling without LGRs
    Authors: AM Lebensohn, R Rohatgi
    Elife, 2018;7(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Differential activities and mechanisms of the four R-Spondins in potentiating Wnt/?-catenin signaling
    Authors: S Park, J Cui, WA Yu, L Wu, K Carmon, QJ Liu
    J. Biol. Chem., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Upregulation of RSPO2-GPR48/LGR4 signaling in papillary thyroid carcinoma contributes to tumor progression
    Authors: YE Kang, JM Kim, KS Kim, JY Chang, M Jung, J Lee, S Yi, HW Kim, JT Kim, K Lee, MJ Choi, SK Kang, SE Lee, HS Yi, BS Koo, M Shong
    Oncotarget, 2017;8(70):114980-114994.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. R-spondin 2 promotes acetylcholine receptor clustering at the neuromuscular junction via Lgr5
    Authors: Hiroaki Nakashima
    Sci Rep, 2016;6(0):28512.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Therapeutic Targeting of Tumor-Derived R-Spondin Attenuates beta-Catenin Signaling and Tumorigenesis in Multiple Cancer Types.
    Authors: Chartier C, Raval J, Axelrod F, Bond C, Cain J, Dee-Hoskins C, Ma S, Fischer M, Shah J, Wei J, Ji M, Lam A, Stroud M, Yen W, Yeung P, Cancilla B, O'Young G, Wang M, Kapoun A, Lewicki J, Hoey T, Gurney A
    Cancer Res, 2016;76(3):713-23.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Bioassay
  10. Aberrantly expressed LGR4 empowers Wnt signaling in multiple myeloma by hijacking osteoblast-derived R-spondins
    Proc. Natl. Acad. Sci. U.S.A, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. RSPO2-LGR5 signaling has tumour-suppressive activity in colorectal cancer.
    Authors: Wu C, Qiu S, Lu L, Zou J, Li W, Wang O, Zhao H, Wang H, Tang J, Chen L, Xu T, Sun Z, Liao W, Luo G, Lu X
    Nat Commun, 2014;5(0):3149.
  12. Interaction with both ZNRF3 and LGR4 is required for the signalling activity of R-spondin.
    Authors: Xie, Yang, Zamponi, Raffaell, Charlat, Olga, Ramones, Melissa, Swalley, Susanne, Jiang, Xiaomo, Rivera, Daniel, Tschantz, William, Lu, Bo, Quinn, Lisa, Dimitri, Chris, Parker, Jefferso, Jeffery, Doug, Wilcox, Sheri K, Watrobka, Mike, LeMotte, Peter, Granda, Brian, Porter, Jeffrey, Myer, Vic E, Loew, Andreas, Cong, Feng
    EMBO Rep, 2013;14(12):1120-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  13. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. ZNRF3 promotes Wnt receptor turnover in an R-spondin-sensitive manner.
    Authors: Hao HX, Xie Y, Zhang Y, Charlat O, Oster E, Avello M, Lei H, Mickanin C, Liu D, Ruffner H, Mao X, Ma Q, Zamponi R, Bouwmeester T, Finan PM, Kirschner MW, Porter JA, Serluca FC, Cong F
    Nature, 2012;485(7397):195-200.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. The role of R-spondin2 in keratinocyte proliferation and epidermal thickening in keloid scarring.
    Authors: Chua AW, Ma D, Gan SU, Fu Z, Han HC, Song C, Sabapathy K, Phan TT
    J. Invest. Dermatol., 2011;131(3):644-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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