Recombinant Human SALM2/LRFN1 Protein, CF

Catalog # Availability Size / Price Qty
5669-SA-050
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Recombinant Human SALM2/LRFN1 Protein, CF Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of Neuro‑2A mouse neuroblastoma cells. When 5 x 104 cells/well are added to rhSALM2 coated plates (2.5 μg/mL, 100 μL/well), approximately 50%-70% will adhere after 30 minutes at 37 °C.  Optimal dilutions should be determined by each laboratory for each application.
Source
Chinese Hamster Ovary cell line, CHO-derived human SALM2/LRFN1 protein
Gln32-Gly534
Accession #
N-terminal Sequence
Analysis
Results not obtained: Gln32 predicted
Predicted Molecular Mass
55.4 kDa
SDS-PAGE
70-80 kDa under reducing conditions

Product Datasheets

5669-SA

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: SALM2/LRFN1

Synaptic adhesion-like molecule 2 (SALM2; also leucine-rich repeat and fibronectin type-III domain-containing protein 1 (Lrfn1) is a 105 kDa member of the Lrfn family of type I transmembrane glycoproteins (1 - 2).  Human SALM2 is synthesized as a 771 amino acid (aa) precursor that contains a 31 aa signal sequence, a 505 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 214 aa cytoplasmic region.  The ECD consists of seven leucine-rich repeats (LRR), an IgC2-like domain, and a fibronectin type-III domain, tandemly aligned in that order (1, 3).  In addition, there are two potential sites for N-linked glycosylation.  The C-terminal region contains an intracellular PDZ binding domain, which is conserved among SALMs 1 - 3, but is absent in SALMs 4 and 5 (4). Mature human SALM2 shares 96% and 95% aa sequence identity with mature mouse and rat SALM2, respectively.  In the developing mouse embryo, the temporal expression profile blot revealed a general increment of expression around E10.5 for SALM2.  Northern blot analysis showed that in rats, SALM2 is strongly expressed in the adult brain and is also present in the adult testis (2). SALM2 localizes to both axons and dendrites (4).  In addition, it co-localizes with both pre- and post-synaptic proteins at excitatory synapses in mature neurons (2, 4).  SALM2, like the other SALMs, promotes neurite outgrowth (4).  In particular, SALM2 promotes an increase in the number of primary processes compared to controls and SALMs 1 and 5 (4).  In addition, SALM2 is an important regulator of the differentiation of excitatory synapses (2). SALM2 may also be involved in synaptic maintenance and other cellular interactions (4).

References
  1. Morimura, N. et al. (2006) Gene 380:72.
  2. Ko, J. et al. (2006) Neuron 50:233.
  3. Wang, C.-Y. et al. (2006) J. Neurosci. 26:2174.
  4. Wang, P.Y. et al. (2008) Mol. Cell. Neurosci. 39:83.
Long Name
Synaptic Adhesion-like Molecule 2/Leucine-rich Repeat and Fibronectin Type III Domain Containing 1
Entrez Gene IDs
57622 (Human); 365222 (Rat)
Alternate Names
KIAA1484; leucine rich repeat and fibronectin type III domain containing 1; leucine-rich repeat and fibronectin type III domain-containing protein 1; LRFN1; SALM2; synaptic adhesion-like molecule 2

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