Recombinant Human SALM2/LRFN1 Protein, CF

R&D Systems | Catalog # 5669-SA

R&D Systems
Discontinued Product
5669-SA has been discontinued. View all SALM2/LRFN1 products.

Key Product Details

Source

CHO

Accession Number

Q9P244

Applications

Bioactivity
View all SALM2/LRFN1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human SALM2/LRFN1 protein
Gln32-Gly534

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Results not obtained: Gln32 predicted

Predicted Molecular Mass

55.4 kDa

SDS-PAGE

70-80 kDa under reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of Neuro‑2A mouse neuroblastoma cells.
When 5 x 104 cells/well are added to rhSALM2 coated plates (2.5 μg/mL, 100 μL/well), approximately 50%-70% will adhere after 30 minutes at 37 °C.  Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation, and Storage

5669-SA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SALM2/LRFN1

Synaptic adhesion-like molecule 2 (SALM2; also leucine-rich repeat and fibronectin type-III domain-containing protein 1 (Lrfn1) is a 105 kDa member of the Lrfn family of type I transmembrane glycoproteins (1 - 2).  Human SALM2 is synthesized as a 771 amino acid (aa) precursor that contains a 31 aa signal sequence, a 505 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 214 aa cytoplasmic region.  The ECD consists of seven leucine-rich repeats (LRR), an IgC2-like domain, and a fibronectin type-III domain, tandemly aligned in that order (1, 3).  In addition, there are two potential sites for N-linked glycosylation.  The C-terminal region contains an intracellular PDZ binding domain, which is conserved among SALMs 1 - 3, but is absent in SALMs 4 and 5 (4). Mature human SALM2 shares 96% and 95% aa sequence identity with mature mouse and rat SALM2, respectively.  In the developing mouse embryo, the temporal expression profile blot revealed a general increment of expression around E10.5 for SALM2.  Northern blot analysis showed that in rats, SALM2 is strongly expressed in the adult brain and is also present in the adult testis (2). SALM2 localizes to both axons and dendrites (4).  In addition, it co-localizes with both pre- and post-synaptic proteins at excitatory synapses in mature neurons (2, 4).  SALM2, like the other SALMs, promotes neurite outgrowth (4).  In particular, SALM2 promotes an increase in the number of primary processes compared to controls and SALMs 1 and 5 (4).  In addition, SALM2 is an important regulator of the differentiation of excitatory synapses (2). SALM2 may also be involved in synaptic maintenance and other cellular interactions (4).

References

  1. Morimura, N. et al. (2006) Gene 380:72.
  2. Ko, J. et al. (2006) Neuron 50:233.
  3. Wang, C.-Y. et al. (2006) J. Neurosci. 26:2174.
  4. Wang, P.Y. et al. (2008) Mol. Cell. Neurosci. 39:83.

Long Name

Synaptic Adhesion-like Molecule 2/Leucine-rich Repeat and Fibronectin Type III Domain Containing 1

Alternate Names

LRFN1

Entrez Gene IDs

57622 (Human); 365222 (Rat)

Gene Symbol

LRFN1

UniProt

Q9P244

Additional SALM2/LRFN1 Products

Product Documents for Recombinant Human SALM2/LRFN1 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human SALM2/LRFN1 Protein, CF

For research use only

Related Research Areas

Immunoglobulin Superfamily CAMs

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