Recombinant Human Semaphorin 4G Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
5840-S4-050
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Recombinant Human Semaphorin 4G Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit the proliferation of HCT‑116 human colorectal carcinoma cells. The ED50 for this effect is 100-400 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human Semaphorin 4G protein
Human Semaphorin 4G
(Val18 - Leu675)
Accession # Q9NTN9
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Val18
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
99 kDa (monomer)
SDS-PAGE
110-120 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5840-S4

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

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Background: Semaphorin 4G

Semaphorin 4G (Sema4G) is the least characterized of the seven known Class 4 transmembrane semaphorin glycoproteins (1). Class 4 semaphorins have multiple roles in cell attraction or repulsion. These include development of nerve pathways in the brain, promoting or inhibiting proliferation, and in some cases organizing immune cell interactions. Receptors include transmembrane plexins, but alternate receptors, such as CD72 for Sema4D and TIM-2 for Sema4A, have been identified (2, 3). The 838 amino acid (aa) human Sema4G precursor contains a 17 aa signal sequence, a 658 aa extracellular domain (ECD) containing sema and C2-type immunoglobulin domains, a 21 aa transmembrane domain, and a 142 aa cytoplasmic domain with one Ser/Thr phosphorylation site (4). The human Sema4G ECD shares 90%, 92%, 93%, 93% and 92% aa identity with mouse, rat, equine, bovine and canine Sema4G, respectively. It also shares 42% aa identity with Sema4C, the most closely related semaphorin. Two additional isoforms have been reported: an 843 aa form contains a 5 aa insert at aa 542, while a 458 aa form diverges at aa 450 near the end of the Sema domain (5). Sema4G mRNA is expressed early in development in the central and peripheral nervous systems and in sensory organs such as retina, cochlea, vomeronasal organ and olfactory epithelium. In adults, Sema4G mRNA is found in predominantly in the liver, but is also detected in the kidneys and brain. Elevated Sema4G mRNA expression was found in mouse kidney during Plasmodium yoelii infection (6). Sema4G was also identified in a screen for genes that are downregulated in human colorectal cancer (7).

References
  1. Li, H. et al. (1999) Mech. Dev. 87:169.
  2. Halloran, M.C. and M.A. Wolman (2006) Curr. Opin. Cell Biol. 18:533.
  3. Suzuki, K. et al. (2008) Nat. Immunol. 9:17.
  4. Swissprot Accession # Q9NTN9.
  5. Entrez Accession # EAW49803, EAW49802.
  6. Lao, A.O.T. et al. (2001) J. Immunol. 166:1945.
  7. Wang, X. et al. (2008) Hepatogastroenterology 55:2039.
Entrez Gene IDs
57715 (Human); 26456 (Mouse); 361764 (Rat)
Alternate Names
KIAA1619FLJ20590; MGC102867; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4G; SEMA4G; Semaphorin 4G; semaphorin-4G

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