Recombinant Human Serpin A7/TBG Protein, CF

Catalog # Availability Size / Price Qty
9564-PI-050
Recombinant Human Serpin A7/TBG Protein Bioactivity
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Recombinant Human Serpin A7/TBG Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Thyroxine-BSA Conjugate is immobilized at 2.5 μg/mL, 100 μL/well, Recombinant Human Serpin A7/TBG binds with an ED50 of 0.4‑2 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Serpin A7/TBG protein
Ala21-Ala415, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ala21
Predicted Molecular Mass
45 kDa
SDS-PAGE
58-66 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9564-PI

Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution Reconstitute at 1 mg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Human Serpin A7/TBG Protein Bioactivity View Larger

When Thyroxine-BSA Conjugate is immobilized at 2.5 μg/mL, 100 μL/well, Recombinant Human Serpin A7/TBG (Catalog # 9564-PI) binds with an ED50 of 0.4-2 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Serpin A7/TBG

Thyroxine-binding globulin (TBG), also known as Serpin A7, is an approximately 44 kDa member of the serpin superfamily of serine protease inhibitors. TBG belongs to the clade A subgroup of serpins which include inflammatory response molecules (1). Human TBG shares 76% sequence identity to mouse TBG. TBG is one of two serpins known to bind and transport hormones. TBG is responsible for binding the majority of thyroxine (T4) in the blood and releasing it through conformational changes in the reactive loop (2). In addition, cleavage of TBG's reactive site loop by proteases present at inflammatory sites causes a targeted and immediate release of thyroxine from TBG (3). TBG levels are modified by estrogen levels (4-6) and may directly predict perinatal syndromal depression in late pregnancy (6). In addition, elevated TBG levels were a reported marker for chronic obstructive pulmonary disease (7) in support of reported endocrine system dysfunction in patients with the disease (8, 9).

References
  1. Law, R. et al. (2006) Genome Biol. 7:216.
  2. Zhou, A. et al. (2006) Proc. Natl. Acad. Sci. USA 103:13321.
  3. Jirasakuldech, B. et al. (2000) J. Clin. Endocrinol. Metab. 85:3996.
  4. Glinoer, D. (1997) Endocr. Rev. 18:404.
  5. Gaberscek, S. and K. Zaletel (2011) Expert Rev. Clin. Immunol. 7:697.
  6. Pedersen, C. et al. (2016) Psychoneuroendocrinology 65:84.
  7. Diao, W. et al. (2017) Int. J. Chron. Obstruct. Pulmon. Dis. 12: 1549.
  8. Terzano, C et al. (2014) Lung 192:103.
  9. Sarinc Ulasli, S. et al. (2013) Multidiscip. Respir. Med. 8:64.
Entrez Gene IDs
6906 (Human); 331535 (Mouse); 81806 (Rat)
Alternate Names
antitrypsin), member 7; FLJ17820; member 7; Serpin A7; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin); T4-binding globulin; TBG; TBGclade A (alpha-1 antiproteinase; thyroxin-binding globulin; thyroxine-binding globulin

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