Recombinant Human Siglec-1 Protein, CF Summary
Ser20-Gln1641, with a C-terminal 6-His tag
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Siglecs are sialic acid specific I-type lectins that belong to the immunoglobulin superfamily. Structurally, they are transmembrane proteins with an N-terminal Ig-like V‑set domain followed by varying numbers of Ig-like C2-set domains (1, 2). Human Siglec-1, also known as sialoadhesin and CD169, is a 175-185 kDa glycoprotein. It contains a 1622 amino acid (aa) extracellular domain (ECD) with one Ig-like V‑set domain and 16 Ig-like C2-set domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain (3). Within the ECD, human Siglec-1 shares approximately 70% aa sequence identity with mouse and rat Siglec-1. Alternate splicing generates a potentially soluble form of the ECD, and a second isoform with a substituted cytoplasmic domain. Siglec-1 expression is restricted to lymph node and splenic macrophages, plus some tissue macrophages (3). The adhesive function of Siglec-1 is supported by the N-terminal Ig-like domain which shows a selectivity for alpha 2,3-linked sialic acid residues (3-5). Siglec-1 binds a number of sialylated molecules including the mannose receptor, MGL1, MUC1, PSGL-1, and different glycoforms of CD43 (6-9). Its binding capacity can be masked by endogenous sialylated molecules (10, 11). The sialylated and sulfated N-linked carbohydrates that modify Siglec-1 itself are required for ligand binding (6, 7). Siglec-1 is expressed on dendritic cells following rhinovirus exposure, and these DC promote T cell anergy (12). It is also induced on circulating monocytes during systemic sclerosis and HIV-1 infection (13 - 15). Siglec-1 can trap HIV-1 particles for trans infection of permissive cells (14).
- Varki, A. and T. Angata (2006) Glycobiology 16:1R.
- Crocker, P.R. et al. (2007) Nat. Rev. Immunol. 7:255.
- Hartnell, A. et al. (2001) Blood 97:288.
- Nath, D. et al. (1995) J. Biol. Chem. 270:26184.
- Crocker, P.R. et al. (1991) EMBO J. 10:1661.
- Martinez-Pomares, L. et al. (1999) J. Biol. Chem. 274:35211.
- Kumamoto, Y. et al. (2004) J. Biol. Chem. 279:49274.
- Nath, D. et al. (1999) Immunology 98:213.
- van den Berg, T.K. et al. (2001) J. Immunol. 166:3637.
- Nakamura, K. et al. (2002) Glycobiology 12:209.
- Barnes, Y.C. et al. (1999) Blood 93:1245.
- Kirchberger, S. et al. (2005) J. Immunol. 175:1145.
- York, M.R. et al. (2007) Arthritis Rheum. 56:1010.
- Rempel, H. et al. (2008) PloS ONE 3:e1967.
- van der Kuyl, A.C. et al. (2007) Plos ONE 2:e257.
Citations for Recombinant Human Siglec-1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 8
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Selective tumor antigen vaccine delivery to human CD169+ antigen-presenting cells using ganglioside-liposomes
Authors: AJ Affandi, J Grabowska, K Olesek, M Lopez Vene, A Barbaria, E Rodríguez, PPG Mulder, HJ Pijffers, M Ambrosini, H Kalay, T O'Toole, ES Zwart, G Kazemier, K Nazmi, FJ Bikker, J Stöckl, AJM van den Ee, TD de Gruijl, G Storm, Y van Kooyk, JMM den Haan
Proc Natl Acad Sci U S A, 2020;0(0):.
Sample Types: Whole Cells
Anti-Siglec-1 antibodies block Ebola viral uptake and decrease cytoplasmic viral entry
Authors: D Perez-Zsol, I Erkizia, M Pino, M García-Gal, MT Martin, S Benet, J Chojnacki, MT Fernández-, D Guerrero, V Urrea, X Muñiz-Trab, L Kremer, J Martinez-P, N Izquierdo-
Nat Microbiol, 2019;0(0):.
Sample Types: Antibody
Applications: Surface Plasmon Resonance (SPR
The plasma biomarker soluble SIGLEC-1 is associated with the type I interferon transcriptional signature, ethnic background and renal disease in systemic lupus erythematosus
Authors: JJ Oliveira, S Karrar, DB Rainbow, CL Pinder, P Clarke, A Rubio Garc, O Al-Assar, K Burling, S Morris, R Stratton, TJ Vyse, LS Wicker, JA Todd, RC Ferreira
Arthritis Res. Ther., 2018;20(1):152.
Applications: ELISA (Standard)
Membrane-wrapped nanoparticles probe divergent roles of GM3 and phosphatidylserine in lipid-mediated viral entry pathways
Authors: F Xu, A Bandara, H Akiyama, B Eshaghi, D Stelter, T Keyes, JE Straub, S Gummuluru, BM Reinhard
Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
Sample Types: Recombinant Protein
An innovative immunotherapeutic strategy for ovarian cancer: CLEC10A and glycomimetic peptides
Authors: LL Eggink, KF Roby, R Cote, J Kenneth Ho
J Immunother Cancer, 2018;6(1):28.
Sample Types: Peptide
Applications: Binding Assay
Broad and direct interaction between TLR and Siglec families of pattern recognition receptors and its regulation by Neu1.
Authors: Chen, Guo-Yun, Brown, Nicholas, Wu, Wei, Khedri, Zahra, Yu, Hai, Chen, Xi, van de Vlekkert, Diantha, D'Azzo, Alessand, Zheng, Pan, Liu, Yang
Sample Types: Cell Lysates
The role of Siglec-1 and SR-BI interaction in the phagocytosis of oxidized low density lipoprotein by macrophages.
Authors: Xiong, Yi-song, Yu, Juan, Li, Chang, Zhu, Lin, Wu, Li-juan, Zhong, Ren-qian
PLoS ONE, 2013;8(3):e58831.
Sample Types: Protein
Siglecs facilitate HIV-1 infection of macrophages through adhesion with viral sialic acids.
Authors: Zou Z, Chastain A, Moir S
PLoS ONE, 2011;6(9):e24559.
Sample Types: Virus
Applications: Surface Plasmon Resonance
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