Recombinant Human Siglec-15 Fc Chimera Protein, CF

  
  • Purity
    >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Neu5Ac(a2-6)GalNAc-PAA-biotin is immobilized onto a Streptavidin-coated plate, Recombinant Human Siglec-15 Fc Chimera binds with an ED50 of 150-900 ng/mL.
  • Source
    Chinese Hamster Ovary cell line, CHO-derived
    Human Siglec-15
    (Phe20-Thr263)
    Accession # Q6ZMC9
    IEGRMD Human IgG1
    (Pro100-Lys330)
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Phe20
  • Predicted Molecular Mass
    53 kDa
  • SDS-PAGE
    53-64 kDa, reducing conditions
9227-SL
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, CHAPS and Trehalose.
Reconstitution Reconstitute at 200 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
When Neu5Ac(a2-6)GalNAc-PAA-biotin is immobilized onto aStreptavidin-coated plate, Recombinant Human Siglec-15 Fc Chimera (Catalog # 9227-SL) bindswith an ED50 of
150-900 ng/mL.
Background: Siglec-15
Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules (1). Mature human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain (2). Within the ECD, human Siglec-15 shares 85% aa sequence identity with mouse and rat Siglec-15. Alternative splicing generates an additional isoform that lacks the signal peptide and first Ig-like domain. Siglec-15 associates with the signaling molecules DAP12 and DAP10 (2-5). It is expressed on osteoclasts, macrophages, and dendritic cells (2-6) and binds to the sialyl-Tn antigen (2, 3, 6). This interaction induces the production of TGF-beta by tumor-associated macrophages (3). Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts (4-6).
  • References:
    1. Humphrey, M.B. and M.C. Nakamura (2015) Clin. Rev. Allergy Immunol. PMID 26573914.
    2. Angata, T. et al. (2007) Glycobiology 17:838.
    3. Takamiya, R. et al. (2013) Glycobiology 23:178.
    4. Kameda, Y. et al. (2013) J. Bone Miner. Res. 28:2463.
    5. Stuible, M. et al. (2014) J. Biol. Chem. 289:6498.
    6. Ishida-Kitagawa, N. et al. (2012) J. Biol. Chem. 287:17493.
  • Long Name:
    Sialic Acid Binding Ig-like Lectin 15
  • Entrez Gene IDs:
    284266 (Human); 620235 (Mouse); 498888 (Rat)
  • Alternate Names:
    CD33 antigen-like 3SIGLEC-15; CD33 molecule-like 3; CD33L3; HsT1361; sialic acid binding Ig-like lectin 15; sialic acid-binding Ig-like lectin 15; Siglec15; Siglec-15
Related Research Areas

Have you used Recombinant Human Siglec-15 Fc Chimera Protein, CF?

Submit a review and receive a $25 amazon gift card if you include an image - $10 Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

    Contact Information

Certificate of Analysis Lookup

The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided

Certificate of Analysis Lookup

Research Areas of Interest
R&D Systems Guarantee

Feedback

Customer Information