Recombinant Human Slit3 (aa 1120-1523) Protein, CF

Catalog # Availability Size / Price Qty
9067-SL-050
Recombinant Human Slit3 (aa 1120-1523)  Protein, CF Bioactivity
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Recombinant Human Slit3 (aa 1120-1523) Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human Slit3, immobilized at 1.25-2.5 μg/mL on a 96-well plate, is able to significantly enhance neurite outgrowth.
Source
Human embryonic kidney cell, HEK293-derived human Slit3 protein
Thr1120-Ser1523, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Thr1120
Predicted Molecular Mass
45 kDa
SDS-PAGE
56-63 kDa, reducing conditions

Product Datasheets

9067-SL

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS and NaCl.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Bioactivity Recombinant Human Slit3 (aa 1120-1523)  Protein, CF Bioactivity View Larger

Recombinant Human Slit3 (Catalog #
9067-SL) Induces Cortical Neurite Outgrowth. A) Untreated E16-E18 embryonic rat cortical neurons. B) Neurite outgrowth in E16-E18 embryonic rat cortical neurons treated with 1.25 µg/ml of Recombinant Human Slit3.

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Background: Slit3

Slit3 is an approximately 200 kDa member of the Slit family of large secreted axon guidance molecules that are ligands for ROBO receptors (1, 2). It is secreted to the extracellular space and also localizes in mitochondria via a mitochondria localization sequence (3). Mature human Slit3 consists of 4 cassettes of leucine-rich repeats (LRR) flanked by LRR N-terminal and C-terminal domains, followed by multiple EGF-like domains, a Laminin G-like domain, and a C-terminal cysteine-rich domain. Alternative splicing generates additional isoforms with a substituted cysteine-rich domain or a deletion in the last EGF-like domain and Laminin G-like domain. During development, Slit3 is expressed in the ventral neural tube, developing sensory organs, limb buds, and developing areas of the limbs in patterns that overlap with but are discrete from Slit1 and Slit2 (1, 2, 4). Axons will not be allowed to recross the floor plate unless all three Slit genes are disrupted, suggesting some overlap in Slit function (5). Slit3 is also expressed in the lung, kidney, skeletal muscle, and heart, both during development and postnatally (6-8). Mice with genetically disrupted Slit3 show abnormalities in diaphragm and kidney development (7, 8). Slit3 is additionally expressed by vascular endothelial cells and smooth muscle cells (9). It binds to both ROBO1 and ROBO4, but it is the ROBO4 interaction that mediates Slit3-induced angiogenesis (9). It also can enhance the chemokine-induced migration of monocytes (10). The related Slit2 protein is cleaved in vivo (at a site conserved in Slit3), and the resulting C-terminal fragment binds Plexin A1 and retains the ability to repel axon migration (4, 11). The corresponding C-terminal fragment of Slit3 is able to bind heparin and neutralize its anti-coagulant activity (12). Within this fragment (aa 1120-1523), human Slit3 shares 93% amino acid identity with mouse and rat Slit3.

References
  1. Blockus, H. and A. Chedotal (2014) Curr. Opin Neurobiol. 27:82.
  2. Gara, R.K. et al. (2015) Drug Discov. Today 20:156.
  3. Little, M. H. et al. (2001) Am. J. Physiol. Cell Physiol. 281:C486.
  4. Brose, K. et al. (1999) Cell 96:795.
  5. Long, H. et al. (2004) Neuron 42:213.
  6. Greenberg, J.M. et al. (2004) Dev. Dyn. 230:350.
  7. Liu, J. et al. (2003) Mech. Dev. 120:1059.
  8. Yuan, W. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5217.
  9. Zhang, B. et al. (2009) Blood 114:4300.
  10. Geutskens, S.B. et al. (2010) J. Immunol. 185:7691.
  11. Delloye-Bourgeois, C. et al. (2015) Nat. Neurosci. 18:36.
  12. Condac, E. et al. (2012) Glycobiology 22:1183.
Long Name
SLIT Homolog 3
Entrez Gene IDs
6586 (Human); 20564 (Mouse); 83467 (Rat)
Alternate Names
KIAA0814; MEGF5; MEGF5Multiple epidermal growth factor-like domains protein 5; Multiple EGF-like domains protein 5; SLIL2; SLIL2FLJ10764; slit homolog 3 (Drosophila); slit homolog 3 protein; SLIT1; slit2; Slit3; Slit-3slit (Drosophila) homolog 3

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