Secretory Leukocyte Protease Inhibitor (SLPI) is a protein found in various secretory fluids, including parotid secretions, bronchial, nasal and cervical mucus, and seminal fluid. The protein is a potent inhibitor of a variety of proteolytic enzymes, including the neutrophil proteases, elastase and cathepsin G and the pancreatic proteases chymotrypsin and trypsin. SLPI is an important component of the antiprotease defense of tissues bathed by secretory fluids. It could be used as a therapeutic agent for treatment of the proteolytic damage of tissues seen in degenerative and inflammatory diseases.
Recombinant Human SLPI Protein, CF
R&D Systems | Catalog # 1274-PI
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Key Product Details
- R&D Systems E. coli-derived Recombinant Human SLPI Protein (1274-PI)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
E. coli
Accession Number
Applications
Inhibition Activity
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Product Specifications
Source
E. coli-derived human SLPI protein
Ser26-Ala132
Ser26-Ala132
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ser26
Predicted Molecular Mass
12 kDa
SDS-PAGE
13 kDa, reducing conditions
Activity
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The IC50 value is <4.0 nM, under the described conditions.
The IC50 value is <4.0 nM, under the described conditions.
Reviewed Applications
Read 1 review rated 5 using 1274-PI in the following applications:
Formulation, Preparation, and Storage
1274-PI
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in sterile PBS.
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| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Calculators
Background: SLPI
Long Name
Secretory Leukocyte Protease Inhibitor
Alternate Names
ALK1, ALPProtease inhibitor WAP4, antileukoproteinase, BLPISeminal proteinase inhibitor, HUSI, HUSI-I, Mucus proteinase inhibitor, secretory leukocyte peptidase inhibitor, Secretory leukocyte protease inhibitor, secretory leukocyte protease inhibitor (antileukoproteinase), WAP four-disulfide core domain protein 4, WAP4HUSI-1, WFDC4MPI
Gene Symbol
SLPI
UniProt
Additional SLPI Products
Product Documents for Recombinant Human SLPI Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human SLPI Protein, CF
For research use only
Related Research Areas
Citations for Recombinant Human SLPI Protein, CF
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Protocols
View specific protocols for Recombinant Human SLPI Protein, CF (1274-PI):
Materials
- Assay Buffer: 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% Brij-35 (v/v), pH 7.5 (TCNB)
- Recombinant Human SLPI (rhSLPI) (Catalog # 1274-PI)
- Trypsin (Sigma, Catalog # T-1426)
- Substrate: MCA-Arg-Pro-Lys-Pro-Val-Glu-NVAL-Trp-Arg-Lys(DNP)-NH2 (Catalog # ES002), 2 mM stock in DMSO
- F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
- Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
- Dilute Trypsin to 0.25 µg/mL in Assay Buffer.
- Prepare a curve of rhSLPI (MW: 11,726 Da) in Assay Buffer. Make the following serial dilutions: 5000, 500, 250, 125, 83.8, 55.6, 37, 24.7, 8.23, and 2.06 nM.
- Mix 25 µL of 0.25 µg/mL Trypsin and 25 µL of the rhSLPI dilutions. Include an enzyme control (in duplicate) of 25 µL Assay Buffer and 25 µL of 0.25 µg/mL Trypsin.
- Incubate reaction mixtures at 37 °C for 60 minutes.
- Dilute Substrate to 20 µM in Assay Buffer.
- After incubation dilute the enzyme inhibitor mixtures five fold in Assay Buffer.
- In a plate load 50 µL of the diluted reaction mixtures to wells.
- Start the reaction by adding 50 µL of 20 µM Substrate to wells.
- Read at excitation and emission wavelengths of 320 nm and 405 nm (top read), respectively in kinetic mode for 5 minutes.
- Derive the IC50 value for rhSLPI from the curve.
- Calculate specific activity for each point using the following formula (if needed):
|
Specific Activity (pmol/min/µg) = |
Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU) |
| amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Derived using calibration standard MCA-Pro-Leu-OH (Bachem, Catalog # M-1975).
Per Well:
- Trypsin: 0.00125 µg
- rhSLPI curve: 250, 25, 12.5, 6.25, 4.19, 2.78, 1.85, 1.235, 0.415, 0.103 and 0 nM
- Substrate: 10 µM
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