ST2, also known as IL-1 R4 and T1, is an Interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses (1, 2). Human ST2 consists of a 310 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 207 aa cytoplasmic domain with an intracellular TIR domain (3, 4). Alternate splicing of the 120 kDa human ST2 generates a soluble 60 kDa isoform that lacks the transmembrane and cytoplasmic regions as well as an isoform that additionally lacks the third Ig‑like domain (4). Within the ECD, human ST2 shares 68% and 64% aa sequence identity with mouse and rat ST2, respectively. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes (5-8). It binds IL-33, a cytokine that is upregulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts (5, 9). IL-33 binding induces the association of ST2 with IL-1R AcP, a shared signaling subunit that also associates with IL-1 RI and IL-1 R rp2 (1, 10, 11). In macrophages, ST2 interferes with signaling from IL-1 RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal (7). In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy (5, 12-14). The soluble ST2 isoform is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma (6, 8, 15). Soluble ST2 functions as a decoy receptor that blocks IL‑33’s ability to signal through transmembrane ST2 (10, 13‑15).
Recombinant Human ST2/IL-33R Fc Chimera Protein, CF
R&D Systems | Catalog # 523-ST
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Key Product Details
- R&D Systems NS0-derived Recombinant Human ST2/IL-33R Fc Chimera Protein (523-ST)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
NS0
Accession Number
Structure / Form
Disulfide-linked homodimer
Applications
Bioactivity
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Product Specifications
Source
Mouse myeloma cell line, NS0-derived human ST2/IL-33R protein
| Human ST2 (Lys19-Phe328) Accession # BAA02233 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
6-His tag |
| N-terminus | C-terminus | ||
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Lys19
Predicted Molecular Mass
62.5 kDa (monomer)
SDS-PAGE
89-102 kDa, reducing conditions
Activity
Measured by its ability to inhibit IL-33-dependent proliferation in D10.G4.1 mouse helper T cells.
The ED50 for this effect is 0.03-0.12 µg/mL.
Optimal dilutions should be determined by each laboratory for each application.
The ED50 for this effect is 0.03-0.12 µg/mL.
Optimal dilutions should be determined by each laboratory for each application.
Reviewed Applications
Read 1 review rated 5 using 523-ST in the following applications:
Formulation, Preparation, and Storage
523-ST
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS.
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| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Calculators
Background: ST2/IL-33R
References
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Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217.
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Gadina, M. and C.A. Jefferies (2007) Science STKE 2007:pe31.
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Tominaga, S. et al. (1992) Biochim. Biophys. Acta 1171:215.
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Li, H. et al. (2000) Genomics 67:284.
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Schmitz, J. et al. (2005) Immunity 23:479.
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Lecart, S. et al. (2002) Eur. J. Immunol. 32:2979.
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Brint, E.K. et al. (2004) Nat. Immunol. 5:373.
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Weinberg, E.O. et al. (2002) Circulation 106:2961.
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Sanada S. et al. (2007) J. Clin. Invest. 117:1538.
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Palmer, G. et al. (2008) Cytokine 42:358.
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Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
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Allakhverdi, Z. et al. (2007) J. Immunol. 179:2051.
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Verri Jr., W.A. et al. (2008) Proc. Natl. Acad. Sci. 105:2723.
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Miller, A.M. et al. (2008) J. Exp. Med. 205:339.
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Hayakawa, H. et al. (2007) J. Biol. Chem. 282:26369.
Long Name
Interleukin 33 Receptor
Alternate Names
DER4, Fit-1, IL-1 R4, IL-1R4, IL-1RL1, IL-33 R, IL-33R, IL1R4, IL33R, Ly84, ST2L, ST2V, T1
Gene Symbol
IL1RL1
UniProt
Additional ST2/IL-33R Products
Product Documents for Recombinant Human ST2/IL-33R Fc Chimera Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human ST2/IL-33R Fc Chimera Protein, CF
For research use only
Related Research Areas
Citations for Recombinant Human ST2/IL-33R Fc Chimera Protein, CF
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Customer Reviews for Recombinant Human ST2/IL-33R Fc Chimera Protein, CF (1)
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Application: Immunoassay StandardVerified Customer | Posted 11/09/2017AF523 was used as both the capture and the detection antibody for the sandwich ELISA for sIL-1R4. The immunoassay standard was 523-ST. Assay had sensitivity of ~90pg/ml.
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Associated Pathways
Innate Lymphoid Cell Differentiation Pathways
Th2 Differentiation Pathway
