Recombinant Human TLR10 Fc Chimera Protein, CF

Name: Recombinant Human TLR10 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 6619-TR
Price: 549 USD
Availability: InStock

R&D Systems | Catalog # 6619-TR

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Product Documents

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Product Specifications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

R&D Systems CHO-derived Recombinant Human TLR10 Fc Chimera Protein (6619-TR)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

Q9BXR5

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity

View all TLR10 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human TLR10 protein

Human TLR10 (Asp20-Thr576) Accession # Q9BXR5	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp20

Predicted Molecular Mass

91.2 kDa (monomer)

SDS-PAGE

115-135 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human TLR10 Fc Chimera is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Human TLR1 Fc Chimera (Catalog # 1484-TR) that produces 50% of the optimal binding response is approximately 0.5‑3 µg/mL.

Formulation, Preparation, and Storage

6619-TR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS. Loading...
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: TLR10

TLR10 (Toll‑like receptor 10, designated CD290) is a 90‑100 kDa type I transmembrane glycoprotein that is a member of the TLR1 subfamily within the TLR family of pathogen-associated molecular pattern (PAMP) receptors (1‑3). Human TLR10 is synthesized as a precursor with a 19 amino acid (aa) signal sequence, an extracellular domain (ECD, aa 20‑576) with 15 or more leucine‑rich repeats (LRR), an LRRCT (LRR C‑terminal) domain and nine potential sites for N‑linked glycosylation, a transmembrane domain, and a cytoplasmic region (aa 598‑811) with a TIR (Toll/IL‑1 receptor) domain (1, 3). Human TLR10 shares 71%, 81%, 82% and 83% aa sequence identity with rat, bovine, porcine and canine TLR10, respectively. The mouse TLR10 gene is nonfunctional (2). TLR10 is expressed on B cells and cell lines, except for early pre‑B cells, and is more highly expressed on activated than resting cells (2, 4). It is also expressed on FOXP3⁺ regulatory T cells, placental trophoblasts, and CD1a⁺ and plasmacytoid dendritic cells (DC), but not on monocyte‑derived DC (2, 5, 6). Among PAMPs, TLR10 is thought to recognize triacylated bacterial lipoproteins (as demonstrated by recognition of the synthetic molecule Pam₃CSK₄), gram positive bacterial peptidoglycans, and other microbially-derived agonists shared with TLR1 (3, 6, 7). It is found as a homodimer or heterodimer with TLR1 or TLR2, associating through a region within the extracellular LRR repeats (2, 3, 7). It interacts directly with MYD88, leading to production of inflammatory cytokines (2, 7).