Recombinant Human TLR7 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
9567-TR-050
The Innovator Series.
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Recombinant Human TLR7 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Bioassay data are not available.
Source
Chinese Hamster Ovary cell line, CHO-derived human TLR7 protein
Human TLR7
(Ala27-Asn839)
Accession # Q9NYK1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Ala27
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
120 kDa
SDS-PAGE
122-150 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9567-TR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity not tested The Innovator Series. View Larger

R&D Systems proteins are almost always sold with a bioassay to indicate activity. However, we recognize that sometimes proteins might be novel, and their bioactivity may not be well understood. In addition, some researchers may wish to use polypeptides to make antibodies. To facilitate the advancement of new science, we now offer our Innovator Series of proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TLR7

TLR7 (Toll-like receptor 7) is a member of the Toll-like receptor family of innate immune receptors. It is mainly expressed in lung, placenta and spleen and belongs to a new sub-family of the TLRs comprising of TLR7, TLR8, an TLR9 (1, 2). Human TLR7 cDNA encodes a 1049 amino acid (aa) precursor that contains a 26 aa signal sequence, an 813 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 189 aa cytoplasmic domain. Mature human TLR7 is 81% aa identical to mature mouse TLR7. Human TLR7 is located in the endosomes and involved in the sequence-dependent sensing of single-stranded RNA, thereby detecting bacterial or viral nucleic acids (3, 4, 5).

References
  1. Du, X. et al. (2000) Eur. Cytokine Netw. 11:362.
  2. Chuang, T.H. and R.J. Ulevitch (2000) Eur. Cytokine Netw. 11:372.
  3. Hornung, V. et al. (2002) J. Immunol. 168:4531.
  4. Melchjorsen, J. et al. (2005) J. Virol. 79:12944.
  5. Gantier, M.P. et al. (2008) J. Immunol. 180:2117.
Long Name
Toll-like Receptor 7
Entrez Gene IDs
51284 (Human); 170743 (Mouse); 317468 (Rat)
Alternate Names
TLR7; toll-like receptor 7

FAQs

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