Recombinant Human TMEM106B Fc Chimera Protein, CF

R&D Systems | Catalog # 11644-TM

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human TMEM106B Fc Chimera Protein (11644-TM)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Structure / Form

Disulfide linked homodimer

Applications

Bioactivity
View all TMEM106B Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human TMEM106B protein
MD Human IgG1
(Pro100-Lys330)		 IEGR Human TREM106B
(Pro118-Gln274)
Accession # NP_001127704.1
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

45 kDa

SDS-PAGE

>60 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human TMEM106B Fc Chimera (Catalog # 11644-TM) binds Recombinant SARS-CoV-2 Spike RBD His-tag (HEK293 Expressed) (Catalog # 10500-CV) with ED50 <2.00 μg/mL.

Scientific Data Images for Recombinant Human TMEM106B Fc Chimera Protein, CF

Recombinant Human TMEM106B Fc Chimera Protein Binding Activity.

Recombinant Human TMEM106B Fc Chimera Protein (Catalog # 11644-TM) binds Recombinant SARS-CoV-2 Spike RBD His-tag (HEK293 Expressed) (10500-CV) with ED50

Formulation, Preparation, and Storage

11644-TM
Formulation Supplied as a 0.2 μm filtered solution in PBS with Trehalose.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: TMEM106B

SARS-CoV-2 is associated with broad tissue tropism, a characteristic often determined by the availability of entry receptors on host cells. TMEM106B, a lysosomal transmembrane protein, can serve as an alternative receptor for SARS-CoV-2 entry into angiotensin-converting enzyme 2 (ACE2)-negative cells. Spike substitution E484D increased TMEM106B binding, thereby enhancing TMEM106B-mediated entry (1). ACE2-independent entry was dependent on endogenous TMEM106B, which binds to the receptor binding domain (RBD). Binding is augmented by mutation E484D (3). TMEM106B-specific monoclonal antibodies blocked SARS-CoV-2 infection, demonstrating a role of TMEM106B in viral entry. Using X-ray crystallography, cryogenic electron microscopy (cryo-EM), and hydrogen-deuterium exchange mass spectrometry (HDX-MS), shows that the luminal domain (LD) of TMEM106B engages the receptor-binding motif of SARS-CoV-2 spike (1). The large loop (RBD@471-491) could anchor TMEM106B, which was then firmly locked by another loop (RBD@444-451) (2). TMEM106B also promotes spike-mediated syncytium formation, suggesting a role of TMEM106B in viral fusion. Findings have identified an ACE2-independent SARS-CoV-2 infection mechanism that involves cooperative interactions with the receptors heparan sulfate and TMEM106B (1).

References

  1. Baggen et al., 2023, Cell 186, 3427–3442.
  2. Xiaoyu et al., 2024 Jan 25, J Phys Chem Lett. 15(3), 671-680.
  3. Prerna et al., 2025, Jan 10;99(2).

Long Name

Transmembrane Protein 106B

Alternate Names

transmembrane protein 106B

Entrez Gene IDs

54664 (Human); 71900 (Mouse); 312132 (Rat)

Gene Symbol

TMEM106B

Additional TMEM106B Products

Product Documents for Recombinant Human TMEM106B Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human TMEM106B Fc Chimera Protein, CF

For research use only

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