Home / TRAIL R2/TNFRSF10B / Recombinant Human TRAIL R2/TNFRSF10B Fc His-tag Protein, CF

Recombinant Human TRAIL R2/TNFRSF10B Fc His-tag Protein, CF

Catalog #: 631-T2/CF
14 Citations Datasheet / COA / SDS
Newer Version Available: 10140-T2
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Discontinued Product

631-T2/CF has been discontinued and is replaced by 10140-T2.

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Citations (14)
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Recombinant Human TRAIL R2/TNFRSF10B Fc His-tag Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L‑929 mouse fibroblast cells treated with TRAIL. The ED50 for this effect is 0.7-2 ng/mL in the presence of 20 ng/mL of rhTRAIL or 6 ng/mL cross-linked rhTRAIL (Catalog # 375-TL and  MAB050).
Source
Mouse myeloma cell line, NS0-derived human TRAIL R2/TNFRSF10B protein
TRAIL R2
(Ala54-Glu182)
Accession # Q6FH58		 IEGRMD Human IgG1
(Pro100-Lys330) 		6-His tag
N-terminus C-terminus
Accession #
Q6FH58
N-terminal Sequence
Analysis
Ala54
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
42 kDa (monomer)
SDS-PAGE
44-50 kDa, reducing conditions

Product Datasheets

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631-T2/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

631-T2/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TRAIL R2/TNFRSF10B

Human TRAIL R2, also called DR5 and TRICK 2 is a type 1, TNF R family, membrane protein which is a receptor for TRAIL (APO2 ligand). In the new TNF superfamily nomenclature, TRAIL R2 is referred to as TNFRSF10B. TRAIL R2 cDNA encodes a 440 amino acid residue precursor protein containing extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. Among TNF receptor family proteins, TRAIL R2 is most closely related to TRAIL R1/DR4, sharing 55% amino acid sequence identity. Binding of trimeric TRAIL to TRAIL R2 induces apoptosis. The induction of apoptosis likely requires oligomerization of the receptor. The human TRAIL R2/Fc chimera neutralizes the ability of TRAIL to induce apoptosis. Besides TRAIL R2, an additional TRAIL R1/DR4, which tranduces apoptosis signaling, and two TRAIL decoy receptors, which antagonize TRAIL-induced apoptosis, have been reported.

References
  1. Chaudhary, P.M. et al. (1997) Immunity 7:821.
  2. Walczak, H. et al. (1997) EMBO J. 16:5386.
  3. Golstein, P. (1997) Curr. Biol. 7:R750.
Long Name
TRAIL Receptor 2
Entrez Gene IDs
8795 (Human); 21933 (Mouse)
Alternate Names
CD262 antigen; CD262; death domain containing receptor for TRAIL/Apo-2L; Death receptor 5; DR5; DR5TRICK2B; Fas-like protein; KILLER/DR5; KILLERapoptosis inducing protein TRICK2A/2B; TNF-related apoptosis-inducing ligand receptor 2; TNFRSF10B; TRAIL R2; TRAIL receptor 2; TRAILR2; TRAIL-R2apoptosis inducing receptor TRAIL-R2; TRAILR2cytotoxic TRAIL receptor-2; TRICK2; TRICK2A; TRICKB; tumor necrosis factor receptor superfamily member 10B; tumor necrosis factor receptor superfamily, member 10b; tumor necrosis factor receptor-like protein ZTNFR9; ZTNFR9

Citations for Recombinant Human TRAIL R2/TNFRSF10B Fc His-tag Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Limiting glutamine utilization activates a GCN2/TRAIL-R2/Caspase-8 apoptotic pathway in glutamine-addicted tumor cells
    Authors: R Yerbes, R Mora-Molin, FJ Fernández-, L Hiraldo, A López-Riva, C Palacios
    Cell Death & Disease, 2022-10-27;13(10):906.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. IFN?beta sensitizes TRAIL?induced apoptosis by upregulation of death receptor 5 in malignant glioma cells
    Authors: S Yoshimura, E Sano, Y Hanashima, S Yamamuro, K Sumi, T Ueda, T Nakayama, H Hara, A Yoshino, Y Katayama
    Oncol. Rep., 2019-10-22;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Targeting of dermal myofibroblasts through death receptor 5 arrests fibrosis in mouse models of scleroderma
    Authors: JS Park, Y Oh, YJ Park, O Park, H Yang, S Slania, LK Hummers, AA Shah, HT An, J Jang, MR Horton, J Shin, HC Dietz, E Song, DH Na, EJ Park, K Kim, KC Lee, VV Roschke, J Hanes, MG Pomper, S Lee
    Nat Commun, 2019-03-08;10(1):1128.
    Species: Human
    Sample Types: Recombinant Protein
  4. Targeted Delivery to Tumor-associated Pericytes via an Affibody with High Affinity for PDGFR? Enhances the in vivo Antitumor Effects of Human TRAIL
    Authors: Z Tao, H Yang, Q Shi, Q Fan, L Wan, X Lu
    Theranostics, 2017-06-01;7(8):2261-2276.
    Applications: Bioassay
  5. Glutamine deprivation sensitizes human breast cancer MDA-MB-231 cells to TRIAL-mediated apoptosis
    Authors: MG Dilshara, JW Jeong, RG Prasad Tha, IM Neelaka Mo, S Lee, SR Park, YH Choi, GY Kim
    Biochem. Biophys. Res. Commun, 2017-02-13;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Metformin Causes G1-Phase Arrest via Down-Regulation of MiR-221 and Enhances TRAIL Sensitivity through DR5 Up-Regulation in Pancreatic Cancer Cells.
    Authors: Tanaka R, Tomosugi M, Horinaka M, Sowa Y, Sakai T
    PLoS ONE, 2015-05-08;10(5):e0125779.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Potential application of temozolomide in mesenchymal stem cell-based TRAIL gene therapy against malignant glioma.
    Authors: Kim, Seong Mu, Woo, Ji Sun, Jeong, Chang Hy, Ryu, Chung He, Jang, Jae-Deog, Jeun, Sin-Soo
    Stem Cells Transl Med, 2014-01-16;3(2):172-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Combination effect of equol and TRAIL against human cervical cancer cells.
    Authors: Kim E, Kim A
    Anticancer Res, 2013-03-01;33(3):903-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Up-regulation of a death receptor renders antiviral T cells susceptible to NK cell-mediated deletion.
    Authors: Peppa, Dimitra, Gill, Upkar S, Reynolds, Gary, Easom, Nicholas, Pallett, Laura J, Schurich, Anna, Micco, Lorenzo, Nebbia, Gaia, Singh, Harsimra, Adams, David H, Kennedy, Patrick, Maini, Mala K
    J Exp Med, 2012-12-17;210(1):99-114.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Potential role of soluble TRAIL in epithelial injury in children with severe RSV infection.
    Authors: Bem RA, Bos AP, Wosten-van Asperen RM, Bruijn M, Lutter R, Sprick MR, van Woensel JB
    Am. J. Respir. Cell Mol. Biol., 2009-07-27;42(6):697-705.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Generation of new TRAIL mutants DR5-A and DR5-B with improved selectivity to death receptor 5.
    Authors: Gasparian ME, Chernyak BV, Dolgikh DA, Yagolovich AV, Popova EN, Sycheva AM, Moshkovskii SA, Kirpichnikov MP
    Apoptosis, 2009-06-01;14(6):778-87.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  12. Regulation of tumour necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in Burkitt's lymphoma cell lines.
    Authors: Mouzakiti A, Packham G
    Br. J. Haematol., 2003-07-01;122(1):61-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Interferon-alpha sensitizes human hepatoma cells to TRAIL-induced apoptosis through DR5 upregulation and NF-kappa B inactivation.
    Authors: Shigeno M, Nakao K, Ichikawa T, Suzuki K, Kawakami A, Abiru S, Miyazoe S, Nakagawa Y, Ishikawa H, Hamasaki K, Nakata K, Ishii N, Eguchi K
    Oncogene, 2003-03-20;22(11):1653-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Inflammatory cytokine regulation of TRAIL-mediated apoptosis in thyroid epithelial cells.
    Authors: Bretz JD, Mezosi E, Giordano TJ, Gauger PG, Thompson NW, Baker JR
    Cell Death Differ., 2002-03-01;9(3):274-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?

    • The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.

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