Recombinant Human TRAIL R2/TNFRSF10B Fc His-tag Protein

R&D Systems | Catalog # 631-T2

R&D Systems
Discontinued Product
631-T2 has been discontinued. An alternative/replacement product is available: 10140-T2. View all TRAIL R2/TNFRSF10B products.

Key Product Details

Source

NS0

Accession Number

Q6FH58

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all TRAIL R2/TNFRSF10B Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human TRAIL R2/TNFRSF10B protein
TRAIL R2
(Ala54-Glu182)
Accession # Q6FH58		 IEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala54

Predicted Molecular Mass

42 kDa (monomer)

SDS-PAGE

44-50 kDa, reducing conditions

Activity

Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L‑929 mouse fibroblast cells treated with TRAIL.
The ED50 for this effect is 0.7-2 ng/mL in the presence of 20 ng/mL of recombinant human (rh) TRAIL or 6 ng/mL cross-linked rhTRAIL (Catalog # 375-TL and MAB050).

Reviewed Applications

Read 3 reviews rated 4.7 using 631-T2 in the following applications:

Formulation, Preparation, and Storage

631-T2
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TRAIL R2/TNFRSF10B

Human TRAIL R2, also called DR5 and TRICK 2 is a type 1, TNF R family, membrane protein which is a receptor for TRAIL (APO2 ligand). In the new TNF superfamily nomenclature, TRAIL R2 is referred to as TNFRSF10B. TRAIL R2 cDNA encodes a 440 amino acid residue precursor protein containing extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. Among TNF receptor family proteins, TRAIL R2 is most closely related to TRAIL R1/DR4, sharing 55% amino acid sequence identity. Binding of trimeric TRAIL to TRAIL R2 induces apoptosis. The induction of apoptosis likely requires oligomerization of the receptor. The human TRAIL R2/Fc chimera neutralizes the ability of TRAIL to induce apoptosis. Besides TRAIL R2, an additional TRAIL R1/DR4, which tranduces apoptosis signaling, and two TRAIL decoy receptors, which antagonize TRAIL-induced apoptosis, have been reported.

References

  1. Chaudhary, P.M. et al. (1997) Immunity 7:821.
  2. Walczak, H. et al. (1997) EMBO J. 16:5386.
  3. Golstein, P. (1997) Curr. Biol. 7:R750.

Long Name

TRAIL Receptor 2

Alternate Names

CD262, DR5, TNFRSF10B, TRAILR2

Entrez Gene IDs

8795 (Human); 21933 (Mouse)

Gene Symbol

TNFRSF10B

UniProt

Q6FH58

Additional TRAIL R2/TNFRSF10B Products

Product Documents for Recombinant Human TRAIL R2/TNFRSF10B Fc His-tag Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human TRAIL R2/TNFRSF10B Fc His-tag Protein

For research use only

Citations for Recombinant Human TRAIL R2/TNFRSF10B Fc His-tag Protein

Customer Reviews for Recombinant Human TRAIL R2/TNFRSF10B Fc His-tag Protein (3)

4.7 out of 5
3 Customer Ratings
5 Stars
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4 Stars
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  • Name: Jenna Nguyen
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 02/08/2019
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    Application: Immunoassay Standard
    Verified Customer | Posted 01/30/2019
  • Recombinant Human TRAIL R2/TNFRSF10B Fc Chimera Protein
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FAQs for Recombinant Human TRAIL R2/TNFRSF10B Fc His-tag Protein

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  • Q:

    This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?

    A: The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.

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