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Recombinant Human UbcH2/UBE2H Dominant Negative, CF

Catalog #: E2-608 Datasheet / COA / SDS

Discontinued Product

E2-608 has been discontinued.
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Recombinant Human UbcH2/UBE2H Dominant Negative, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
The Ubiquitin-conjugating (E2) enzyme family receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Recombinant Human UbcH2/UBE2H Dominant Negative is catalytically inactive and is ideal for use as a negative control. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UbcH2/UBE2H Dominant Negative concentration of 0.1-1 μM.
Source
E. coli-derived human UbcH2/UBE2H protein
Accession # P62256
Accession #
P62256

Product Datasheets

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E2-608

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-608

Formulation Supplied as a solution in HEPES, NaCl, DTT and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: UbcH2/UBE2H

Ubiquitin-conjugating Enzyme H2 (UbcH2), also known as Ubiquitin-conjugating Enzyme E2H (UBE2H), is a member of the Ubiquitin-conjugating (E2) enzyme family (1). UbcH2/UBE2H has a predicted molecular weight of 20.5 kDa. The human protein shares 100% amino acid sequence identity with the mouse and rat orthologs. Human UbcH2/UBE2H is highly expressed in skeletal and cardiac muscle, shows significant expression levels in kidney and placenta, and is expressed at low levels in liver, brain, lung, and spleen (2). TNF-alpha induces UbcH2/UBE2H expression via NF-kappa B, and SCL/Tal1 upregulates the expression of UbcH2/UBE2H during erythroid differentiation (2,3). Pathologically, a single nucleotide polymorphism within UbcH2/UBE2H is associated with autism (4). Furthermore, UbcH2/UBE2H has been reported as a putative oncogene for liver tumorigenesis (5). The active site of this protein was chemically inactivated for use as a negative or competitive control.

References
  1. Kaiser, P. et al. (1994) J. Biol. Chem. 269:8797.
  2. Li, Y.P. et al. (2003) FASEB J. 17:1048.
  3. Lausen, J. et al. (2010) J. Biol. Chem. 285:5338.
  4. Vourc'h, P. et al. (2003) Psychiatr. Genet. 13:221.
  5. Keng, V.W. et al. (2009) Nat. Biotechnol. 27:264.
 
Entrez Gene IDs
7328 (Human); 22214 (Mouse)
Alternate Names
E2-14K; E2-20K; EC 6.3.2.19; UBC8; UBC8E2-20K; UBCH; UbcH2; UBE2H; Ubiquitin carrier protein H; ubiquitin-conjugating enzyme E2 H; Ubiquitin-conjugating enzyme E2-20K; ubiquitin-conjugating enzyme E2H (homologous to yeast UBC8); ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast); Ubiquitin-protein ligase H

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