Recombinant Human UbcH5a/UBE2D1 Protein, CF

(19 citations)   
  • Purity
    >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
  • Activity
    Recombinant Human UbcH5a/UBE2D1 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UbcH5a/UBE2D1 concentration of 0.1-1 μM.
  • Source
    E. coli-derived
  • Accession #
  • Predicted Molecular Mass
    17 kDa
E2-616
 
Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Background: UbcH5a/UBE2D1

Ubiquitin-conjugating Enzyme H5a (UbcH5a), also known as Ubiquitin-conjugating Enzyme E2D 1 (UBE2D1), is a ubiquitously expressed protein that is related to Stimulator of Iron Transport (SFT) (1,2). Human UbcH5a/UBE2D1 has a predicted molecular weight of 17 kDa and shares 89% and 88% amino acid (aa) sequence identity with the related family members UbcH5b and UbcH5c, respectively (3). Human UbcH5a/UBE2D1 shares 100% aa sequence identity with the mouse and rat orthologs. UbcH5a/UBE2D1 has a conserved E2 catalytic core domain that contains an active site cysteine residue, and it interacts with a variety of HECT and RING finger Ubiquitin ligases (E3) to mediate the ubiquitination of specific target proteins (4). UbcH5a/UBE2D1 interacts with the E3, E6-AP, to conjugate Ubiquitin to the tumor suppressor, p53 (1). Additional protein targets of UbcH5a/UBE2D1 include c-Fos, RIP1, and HIF-1 (5,6). Pathologically, UbcH5a/UBE2D1 is implicated in protein degradation during cancer and immune responses (7).

  • References:
    1. Scheffner, M. et al. (1994) Proc. Natl. Acad. Sci. USA 91:8797.
    2. Gehrke, S. et al. (2003) Blood 101:3288.
    3. Jensen, J. et al. (1995) J. Biol. Chem. 270:30408.
    4. Lorick, K. et al. (2005) Methods Enzymol. 398:54.
    5. Stancovski, I. et al. (1995) Mol. Cell Biol. 15:7106.
    6. Dynek, J. et al. (2010) EMBO J. 29:4128.
    7. Vanni, E. et al. (2012) J. Virol. 86:6323.
  • Entrez Gene IDs:
    7321 (Human); 216080 (Mouse); 361831 (Rat)
  • Alternate Names:
    E2(17)KB1; Stimulator of Fe transportUBC4/5 homolog; UBC4/5; UBC5A; UbcH5; UbcH5a; UBCH5AEC 6.3.2.19; UBCH5SFT; UBE2D1; Ubiquitin carrier protein D1; ubiquitin-conjugating enzyme E2 D1; Ubiquitin-conjugating enzyme E2(17)KB 1; Ubiquitin-conjugating enzyme E2-17 kDa 1; ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, yeast); Ubiquitin-protein ligase D1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. Ubiquitin-dependent regulation of Cdc42 by XIAP
    Authors: A Murali, J Shin, H Yurugi, A Krishnan, M Akutsu, A Carpy, B Macek, K Rajalingam
    Cell Death Dis, 2017;8(6):e2900.
    Species: Human
    Sample Type: Purified Proteins
    Application: Bioassay
  2. Keap1/Cullin3 Modulates p62/SQSTM1 Activity via UBA Domain Ubiquitination
    Authors: Y Lee, TF Chou, SK Pittman, AL Keith, B Razani, CC Weihl
    Cell Rep, 2017;19(1):188-202.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  3. pSILAC mass spectrometry reveals ZFP91 as IMiD-dependent substrate of the CRL4(CRBN) ubiquitin ligase
    Authors: J An, CM Ponthier, R Sack, J Seebacher, MB Stadler, KA Donovan, ES Fischer
    Nat Commun, 2017;8(0):15398.
    Species: N/A
    Sample Type: Proteins
    Application: Bioassay
  4. Ubiquitin-dependent regulation of Cdc42 by XIAP
    Authors: A Murali, J Shin, H Yurugi, A Krishnan, M Akutsu, A Carpy, B Macek, K Rajalingam
    Cell Death Dis, 2017;8(6):e2900.
    Species: N/A
    Sample Type: Proteins
    Application: Bioassay
  5. Internally tagged ubiquitin: a tool to identify linear polyubiquitin-modified proteins by mass spectrometry
    Authors: K Kliza, C Taumer, I Pinzuti, M Franz-Wach, S Kunzelmann, B Stieglitz, B Macek, K Husnjak
    Nat. Methods, 2017;0(0):.
    Species: N/A
    Sample Type: Protein
    Application: Bioassay
  6. DNA damage and S phase-dependent E2F1 stabilization requires the cIAP1 E3-ubiquitin ligase and is associated with K63-poly-ubiquitination on lysine 161/164 residues
    Authors: V Glorian, J Allègre, J Berthelet, B Dumetier, PM Boutanquoi, N Droin, C Kayaci, J Cartier, S Gemble, G Marcion, D Gonzalez, R Boidot, C Garrido, O Michaud, E Solary, L Dubrez
    Cell Death Dis, 2017;8(5):e2816.
    Species: Human
    Sample Type: Recombinant Protein
    Application: Bioassay
  7. DNA damage and S phase-dependent E2F1 stabilization requires the cIAP1 E3-ubiquitin ligase and is associated with K63-poly-ubiquitination on lysine 161/164 residues
    Authors: V Glorian, J Allègre, J Berthelet, B Dumetier, PM Boutanquoi, N Droin, C Kayaci, J Cartier, S Gemble, G Marcion, D Gonzalez, R Boidot, C Garrido, O Michaud, E Solary, L Dubrez
    Cell Death Dis, 2017;8(5):e2816.
    Species: Bacteria - E. Coli
    Sample Type: Protein
    Application: Bioassay
  8. Keap1/Cullin3 Modulates p62/SQSTM1 Activity via UBA Domain Ubiquitination
    Authors: Y Lee, TF Chou, SK Pittman, AL Keith, B Razani, CC Weihl
    Cell Rep, 2017;19(1):188-202.
    Species: Mouse
    Sample Type: Protein
    Application: Bioassay
  9. Role of SUMO activating enzyme in cancer stem cell maintenance and self-renewal
    Nat Commun, 2016;7(0):12326.
    Species: N/A
    Sample Type: Recombinant Protein
    Application: Bioassay
  10. The adenovirus E4-ORF3 protein functions as a SUMO E3 ligase for TIF-1? sumoylation and poly-SUMO chain elongation
    Proc Natl Acad Sci USA, 2016;113(24):6725-30.
    Species: Human
    Sample Type: Protein
    Application: Bioassay
  11. A novel cereblon modulator recruits GSPT1 to the CRL4(CRBN) ubiquitin ligase
    Authors: Mary E Matyskiela
    Nature, 2016;0(0):.
    Species: N/A
    Sample Type: Protein
    Application: Bioassay
  12. Identification of a novel K311 ubiquitination site critical for androgen receptor transcriptional activity
    Nucleic Acids Res., 2016;0(0):.
    Application: Bioassay
  13. Cellular inhibitor of apoptosis (cIAP)-mediated ubiquitination of phosphofurin acidic cluster sorting protein 2 (PACS-2) negatively regulates tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) cytotoxicity.
    Authors: Guicciardi, Maria Eu, Werneburg, Nathan W, Bronk, Steven F, Franke, Adrian, Yagita, Hideo, Thomas, Gary, Gores, Gregory
    PLoS ONE, 2014;9(3):e92124.
    Species: N/A
    Sample Type: Recombinant Protein
    Application: Bioassay
  14. A HECT ubiquitin-protein ligase as a novel candidate gene for altered quinine and quinidine responses in Plasmodium falciparum.
    Authors: Sanchez C, Liu C, Mayer S, Nurhasanah A, Cyrklaff M, Mu J, Ferdig M, Stein W, Lanzer M
    PLoS Genet, 2014;10(5):e1004382.
    Species: Plasmodium falciparum
    Sample Type: Protein
    Application: Bioassay
  15. Pin1 modulates ERalpha levels in breast cancer through inhibition of phosphorylation-dependent ubiquitination and degradation.
    Authors: Rajbhandari P, Schalper K, Solodin N, Ellison-Zelski S, Ping Lu K, Rimm D, Alarid E
    Oncogene, 2014;33(11):1438-47.
    Species: N/A
    Sample Type: Recombinant Protein
    Application: Bioassay
  16. The ubiquitin ligase ASB4 promotes trophoblast differentiation through the degradation of ID2.
    Authors: Townley-Tilson, W H Davi, Wu, Yaxu, Ferguson, James E, Patterson, Cam
    PLoS ONE, 2014;9(2):e89451.
    Species: Human
    Sample Type: Whole Cells
    Application: Ubiquitination
  17. C. elegans ring finger protein RNF-113 is involved in interstrand DNA crosslink repair and interacts with a RAD51C homolog.
    Authors: Lee, Hyojin, Alpi, Arno F, Park, Mi So, Rose, Ann, Koo, Hyeon-So
    PLoS ONE, 2013;8(3):e60071.
    Species: Human
    Sample Type: Recombinant Protein
    Application: EnzAct
  18. A novel role for inhibitor of apoptosis (IAP) proteins as regulators of endothelial barrier function by mediating RhoA activation.
    Authors: Hornburger M, Mayer B, Leonhardt S, Willer E, Zahler S, Beyerle A, Rajalingam K, Vollmar A, Furst R
    FASEB J, 2013;28(4):1938-46.
    Species: Human
    Sample Type: Recombinant Protein
    Application: Ubiquination
  19. Characterization of ML-IAP protein stability and physiological role in vivo.
    Authors: Varfolomeev, Eugene, Moradi, Elham, Dynek, Jasmin N, Zha, Jiping, Fedorova, Anna V, Deshayes, Kurt, Fairbrother, Wayne J, Newton, Kim, Le Couter, Jennifer, Vucic, Domagoj
    Biochem J, 2012;447(3):427-36.
    Species: Human
    Sample Type: Protein
    Application: Bioassay
Expand to show all 19 Citations

Have you used Recombinant Human UbcH5a/UBE2D1 Protein, CF?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

    Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Feedback

Customer Information