Recombinant Human UbcH5a/UBE2D1 Protein, CF Summary
Product Specifications
Met1 - Met147
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Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E2-616
Formulation | Supplied as a 0.2 μm filtered solution in HEPES, NaCl, TCEP and Glycerol. |
Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: UbcH5a/UBE2D1
Ubiquitin-conjugating Enzyme H5a (UbcH5a), also known as Ubiquitin-conjugating Enzyme E2D 1 (UBE2D1), is a ubiquitously expressed protein that is related to Stimulator of Iron Transport (SFT) (1,2). Human UbcH5a/UBE2D1 has a predicted molecular weight of 17 kDa and shares 89% and 88% amino acid (aa) sequence identity with the related family members UbcH5b and UbcH5c, respectively (3). Human UbcH5a/UBE2D1 shares 100% aa sequence identity with the mouse and rat orthologs. UbcH5a/UBE2D1 has a conserved E2 catalytic core domain that contains an active site cysteine residue, and it interacts with a variety of HECT and RING finger Ubiquitin ligases (E3) to mediate the ubiquitination of specific target proteins (4). UbcH5a/UBE2D1 interacts with the E3, E6-AP, to conjugate Ubiquitin to the tumor suppressor, p53 (1). Additional protein targets of UbcH5a/UBE2D1 include c-Fos, RIP1, and HIF-1 (5,6). Pathologically, UbcH5a/UBE2D1 is implicated in protein degradation during cancer and immune responses (7).
- Scheffner, M. et al. (1994) Proc. Natl. Acad. Sci. USA 91:8797.
- Gehrke, S. et al. (2003) Blood 101:3288.
- Jensen, J. et al. (1995) J. Biol. Chem. 270:30408.
- Lorick, K. et al. (2005) Methods Enzymol. 398:54.
- Stancovski, I. et al. (1995) Mol. Cell Biol. 15:7106.
- Dynek, J. et al. (2010) EMBO J. 29:4128.
- Vanni, E. et al. (2012) J. Virol. 86:6323.
Citations for Recombinant Human UbcH5a/UBE2D1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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DELTEX E3 ligases ubiquitylate ADP-ribosyl modification on protein substrates
Authors: K Zhu, MJ Suskiewicz, A Hloušek-Ka, H Meudal, A Miko?, V Aucagne, D Ahel, I Ahel
Science Advances, 2022;8(40):eadd4253.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
The E3 ubiquitin ligase MG53 inhibits hepatocellular carcinoma by targeting RAC1 signaling
Authors: X Ma, X Ma, L Zhu, Y Zhao, M Chen, T Li, Y Lin, D Ma, C Sun, L Han
Oncogenesis, 2022;11(1):40.
Species: Human
Sample Types: Recombinant Proteins
Applications: Bioassay -
Membrane Targeting and GTPase Activity of Rab7 Are Required for Its Ubiquitination by RNF167
Authors: K Ghilarducc, VC Cabana, A Harake, L Cappadocia, MP Lussier
International Journal of Molecular Sciences, 2022;23(14):.
Species: Human
Sample Types: Recombinant Proteins
Applications: Bioassay -
Induction of zinc finger protein RNF6 auto-ubiquitination for the treatment of myeloma and chronic myeloid leukemia
Authors: H Zhuang, Y Ren, C Mao, Y Zhong, Z Zhang, B Cao, Y Zhang, J Huang, G Xu, Z Huang, Y Xu, X Mao
The Journal of Biological Chemistry, 2022;298(9):102314.
Species: N/A
Sample Types: Recombinant Protein
Applications: Bioassay -
Regulation of p27 (Kip1) by Ubiquitin E3 Ligase RNF6
Authors: D Deshmukh, J Xu, X Yang, H Shimelis, S Fang, Y Qiu
Pharmaceutics, 2022;14(4):.
Species: N/A
Sample Types: Recombinant Protein
Applications: Bioassay -
Role of ubiquitin-protein ligase UBR5 in the disassembly of mitotic checkpoint complexes
Authors: S Kaisari, S Miniowitz-, D Sitry-Shev, P Shomer, G Kozlov, K Gehring, A Hershko
Proceedings of the National Academy of Sciences of the United States of America, 2022;119(9):.
Species: Human
Sample Types: Cell Lysates
Applications: Bioassay -
Proteasomal degradation of the tumour suppressor FBW7 requires branched ubiquitylation by TRIP12
Authors: OM Khan, J Almagro, JK Nelson, S Horswell, V Encheva, KS Keyan, BE Clurman, AP Snijders, A Behrens
Nature Communications, 2021;12(1):2043.
Species: Human
Sample Types: Protein
Applications: Bioassay -
K63-linked ubiquitination of DYRK1A by TRAF2 alleviates Sprouty 2-mediated degradation of EGFR
Authors: P Zhang, Z Zhang, Y Fu, Y Zhang, MP Washburn, L Florens, M Wu, C Huang, Z Hou, M Mohan
Cell Death & Disease, 2021;12(6):608.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
BRCA1-BARD1 regulates transcription through BRD4 in Xenopus nucleoplasmic extract
Authors: JK Barrows, G Fullbright, DT Long
Nucleic Acids Research, 2021;0(0):.
Species: N/A
Sample Types: Plasmid
Applications: Ubiquitination -
PARylation prevents the proteasomal degradation of topoisomerase I DNA-protein crosslinks and induces their deubiquitylation
Authors: Y Sun, J Chen, SN Huang, YP Su, W Wang, K Agama, S Saha, LM Jenkins, JM Pascal, Y Pommier
Nature Communications, 2021;12(1):5010.
Applications: Bioassay -
Small-molecule inhibitors targeting Polycomb repressive complex 1 RING domain
Authors: S Shukla, W Ying, F Gray, Y Yao, ML Simes, Q Zhao, H Miao, HJ Cho, P González-A, A Winkler, G Lund, T Purohit, E Kim, X Zhang, JM Ray, S He, C Nikolaidis, J Ndoj, J Wang, ? Jaremko, M Jaremko, RJH Ryan, ML Guzman, J Grembecka, T Cierpicki
Nature Chemical Biology, 2021;17(7):784-793.
Species: Human
Sample Types: Whole Cells
Applications: Ubiquitination -
FBXL20 promotes breast cancer malignancy by inhibiting apoptosis through degradation of PUMA and BAX
Authors: RK Manne, Y Agrawal, SK Malonia, S Banday, S Edachery, A Patel, A Kumar, P Shetty, MK Santra
The Journal of Biological Chemistry, 2021;0(0):101253.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
The E3 ubiquitin ligase component, Cereblon, is an evolutionarily conserved regulator of Wnt signaling
Authors: C Shen, A Nayak, LR Neitzel, AA Adams, M Silver-Ise, LM Sawyer, H Benchabane, H Wang, N Bunnag, B Li, DT Wynn, F Yang, M Garcia-Con, CH Williams, S Dakshanamu, CC Hong, NG Ayad, AJ Capobianco, Y Ahmed, E Lee, DJ Robbins
Nature Communications, 2021;12(1):5263.
Species: Human
Sample Types: Cell Lysates
Applications: Bioassay -
ANKRD13a controls early cell-death checkpoint by interacting with RIP1 independent of NF-kappaB
Authors: M Won, KA Park, S Kim, E Ju, Y Ko, H Yoo, H Ro, J Lee, J Oh, EG Lee, SY Kim, SW Nam, HM Shen, MK Yeo, JM Kim, GM Hur
Cell Death and Differentiation, 2021;0(0):.
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Crystal structure of the SALL4-pomalidomide-cereblon-DDB1 complex
Authors: ME Matyskiela, T Clayton, X Zheng, C Mayne, E Tran, A Carpenter, B Pagarigan, J McDonald, M Rolfe, LG Hamann, G Lu, PP Chamberlai
Nat. Struct. Mol. Biol., 2020;27(4):319-322.
Species: Spodoptera frugiperda
Sample Types: Whole Cells
Applications: Ubiquitination -
The CDK inhibitor CR8 acts as a molecular glue degrader that depletes cyclin K
Authors: M S?abicki, Z Kozicka, G Petzold, YD Li, M Manojkumar, RD Bunker, KA Donovan, QL Sievers, J Koeppel, D Suchyta, AS Sperling, EC Fink, JA Gasser, LR Wang, SM Corsello, RS Sellar, M Jan, D Gillingham, C Scholl, S Fröhling, TR Golub, ES Fischer, NH Thomä, BL Ebert
Nature, 2020;0(0):.
Species: N/A
Sample Types: Recombinant Protein
Applications: Bioassay -
CHIP ubiquitylates NOXA and induces its lysosomal degradation in response to DNA damage
Authors: MC Albert, K Brinkmann, W Pokrzywa, SD Günther, M Krönke, T Hoppe, H Kashkar
Cell Death & Disease, 2020;11(9):740.
Species: Human
Sample Types: Whole Cells
Applications: Ubiquitylation -
PHF7 Modulates BRDT Stability and Histone-to-Protamine Exchange during Spermiogenesis
Authors: CR Kim, T Noda, H Kim, G Kim, S Park, Y Na, S Oura, K Shimada, I Bang, JY Ahn, YR Kim, SK Oh, HJ Choi, JS Kim, I Jung, H Lee, Y Okada, M Ikawa, SH Baek
Cell Rep, 2020;32(4):107950.
Species: Mouse
Sample Types: Protein
Applications: Bioassay -
DBC1 Regulates p53 Stability via Inhibition of CBP-Dependent p53 Polyubiquitination
Authors: OE Akande, PK Damle, M Pop, NE Sherman, BB Szomju, LV Litovchick, SR Grossman
Cell Rep, 2019;26(12):3323-3335.e4.
Applications: Bioassay -
Competitive ubiquitination activates the tumor suppressor p53
Authors: X Li, M Guo, L Cai, T Du, Y Liu, HF Ding, H Wang, J Zhang, X Chen, C Yan
Cell Death Differ., 2019;0(0):.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
Aryl Sulfonamides Degrade RBM39 and RBM23 by Recruitment to CRL4-DCAF15
Authors: TC Ting, M Goralski, K Klein, B Wang, J Kim, Y Xie, D Nijhawan
Cell Rep, 2019;29(6):1499-1510.e6.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
Human papillomavirus E7 oncoprotein targets RNF168 to hijack the host DNA damage response
Authors: J Sitz, SA Blanchet, SF Gameiro, E Biquand, TM Morgan, M Galloy, J Dessapt, EG Lavoie, A Blondeau, BC Smith, JS Mymryk, CA Moody, A Fradet-Tur
Proc. Natl. Acad. Sci. U.S.A., 2019;116(39):19552-19562.
Species: Virus - HPV
Sample Types: Recombinant Protein
Applications: Bioassay -
Mechanism for recycling tRNAs on stalled ribosomes
Authors: MCJ Yip, AFA Keszei, Q Feng, V Chu, MJ McKenna, S Shao
Nat. Struct. Mol. Biol., 2019;0(0):.
Species: Human
Sample Types: Recombinant Protein
Applications: Ubiquitination -
Suppression of autophagy during mitosis via CUL4-RING ubiquitin ligases-mediated WIPI2 polyubiquitination and proteasomal degradation
Authors: G Lu, J Yi, A Gubas, YT Wang, Y Wu, Y Ren, M Wu, Y Shi, C Ouyang, HWS Tan, T Wang, L Wang, ND Yang, S Deng, D Xia, RH Chen, SA Tooze, HM Shen
Autophagy, 2019;0(0):1-18.
Applications: Bioassay -
E3 ubiquitin ligase RNF123 targets lamin B1 and lamin-binding proteins
Authors: R Khanna, V Krishnamoo, VK Parnaik
FEBS J., 2018;0(0):.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
MIB-1 Is Required for Spermatogenesis and Facilitates LIN-12 and GLP-1 Activity inCaenorhabditis elegans
Authors: M Ratliff, KL Hill-Harfe, EJ Gleason, H Ling, TL Kroft, SW L'Hernault
Genetics, 2018;0(0):.
Species: N/A
Sample Types: Recombinant Protein
Applications: Bioassay -
Regulation of a distinct activated RIPK1 intermediate bridging complex I and complex II in TNF?-mediated apoptosis
Authors: P Amin, M Florez, A Najafov, H Pan, J Geng, D Ofengeim, SA Dziedzic, H Wang, VJ Barrett, Y Ito, MJ LaVoie, J Yuan
Proc. Natl. Acad. Sci. U.S.A., 2018;115(26):E5944-E5953.
Applications: Bioassay -
Cryo-EM structures and dynamics of substrate-engaged human 26S proteasome
Authors: Y Dong, S Zhang, Z Wu, X Li, WL Wang, Y Zhu, S Stoilova-M, Y Lu, D Finley, Y Mao
Nature, 2018;0(0):.
Species: Human
Sample Types: Protein
Applications: Bioassay -
PI5P4K? functions in DTX1-mediated Notch signaling
Authors: L Zheng, SD Conner
Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
CNPY2 inhibits MYLIP-mediated AR protein degradation in prostate cancer cells
Authors: S Ito, A Ueno, T Ueda, H Nakagawa, H Taniguchi, N Kayukawa, A Fujihara-I, F Hongo, K Okihara, O Ukimura
Oncotarget, 2018;9(25):17645-17655.
Applications: Bioassay -
SALL4 mediates teratogenicity as a thalidomide-dependent cereblon substrate
Authors: ME Matyskiela, S Couto, X Zheng, G Lu, J Hui, K Stamp, C Drew, Y Ren, M Wang, A Carpenter, CW Lee, T Clayton, W Fang, CC Lu, M Riley, P Abdubek, K Blease, J Hartke, G Kumar, R Vessey, M Rolfe, LG Hamann, PP Chamberlai
Nat. Chem. Biol., 2018;0(0):.
Species: Human
Sample Types: Recombinant Protein
Applications: Ubiquitination -
Internally tagged ubiquitin: a tool to identify linear polyubiquitin-modified proteins by mass spectrometry
Authors: K Kliza, C Taumer, I Pinzuti, M Franz-Wach, S Kunzelmann, B Stieglitz, B Macek, K Husnjak
Nat. Methods, 2017;0(0):.
Species: N/A
Sample Types: Protein
Applications: Bioassay -
pSILAC mass spectrometry reveals ZFP91 as IMiD-dependent substrate of the CRL4(CRBN) ubiquitin ligase
Authors: J An, CM Ponthier, R Sack, J Seebacher, MB Stadler, KA Donovan, ES Fischer
Nat Commun, 2017;8(0):15398.
Species: N/A
Sample Types: Protein
Applications: Bioassay -
Thymine DNA glycosylase modulates DNA damage response and gene expression by base excision repair-dependent and independent mechanisms
Authors: T Nakamura, K Murakami, H Tada, Y Uehara, J Nogami, K Maehara, Y Ohkawa, H Saitoh, H Nishitani, T Ono, R Nishi, M Yokoi, W Sakai, K Sugasawa
Genes Cells, 2017;0(0):.
Applications: Bioassay -
Keap1/Cullin3 Modulates p62/SQSTM1 Activity via UBA Domain Ubiquitination
Authors: Y Lee, TF Chou, SK Pittman, AL Keith, B Razani, CC Weihl
Cell Rep, 2017;19(1):188-202.
Species: Mouse
Sample Types: Protein
Applications: Bioassay -
Ubiquitin Modification by the E3 Ligase/ADP-Ribosyltransferase Dtx3L/Parp9
Authors: CS Yang, K Jividen, A Spencer, N Dworak, L Ni, LT Oostdyk, M Chatterjee, B Ku?mider, B Reon, M Parlak, V Gorbunova, T Abbas, E Jeffery, NE Sherman, BM Paschal
Mol. Cell, 2017;66(4):503-516.e5.
Species: Human
Sample Types: Protein
Applications: Bioassay -
Ubiquitin Modification by the E3 Ligase/ADP-Ribosyltransferase Dtx3L/Parp9
Authors: CS Yang, K Jividen, A Spencer, N Dworak, L Ni, LT Oostdyk, M Chatterjee, B Ku?mider, B Reon, M Parlak, V Gorbunova, T Abbas, E Jeffery, NE Sherman, BM Paschal
Mol. Cell, 2017;66(4):503-516.e5.
Applications: Bioassay -
Keap1/Cullin3 Modulates p62/SQSTM1 Activity via UBA Domain Ubiquitination
Authors: Y Lee, TF Chou, SK Pittman, AL Keith, B Razani, CC Weihl
Cell Rep, 2017;19(1):188-202.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Ubiquitin-dependent regulation of Cdc42 by XIAP
Authors: A Murali, J Shin, H Yurugi, A Krishnan, M Akutsu, A Carpy, B Macek, K Rajalingam
Cell Death Dis, 2017;8(6):e2900.
Species: N/A
Sample Types: Protein
Applications: Bioassay -
DNA damage and S phase-dependent E2F1 stabilization requires the cIAP1 E3-ubiquitin ligase and is associated with K63-poly-ubiquitination on lysine 161/164 residues
Authors: V Glorian, J Allègre, J Berthelet, B Dumetier, PM Boutanquoi, N Droin, C Kayaci, J Cartier, S Gemble, G Marcion, D Gonzalez, R Boidot, C Garrido, O Michaud, E Solary, L Dubrez
Cell Death Dis, 2017;8(5):e2816.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
Ubiquitin-dependent regulation of Cdc42 by XIAP
Authors: A Murali, J Shin, H Yurugi, A Krishnan, M Akutsu, A Carpy, B Macek, K Rajalingam
Cell Death Dis, 2017;8(6):e2900.
Species: Human
Sample Types: Protein
Applications: Bioassay -
DNA damage and S phase-dependent E2F1 stabilization requires the cIAP1 E3-ubiquitin ligase and is associated with K63-poly-ubiquitination on lysine 161/164 residues
Authors: V Glorian, J Allègre, J Berthelet, B Dumetier, PM Boutanquoi, N Droin, C Kayaci, J Cartier, S Gemble, G Marcion, D Gonzalez, R Boidot, C Garrido, O Michaud, E Solary, L Dubrez
Cell Death Dis, 2017;8(5):e2816.
Species: Bacteria - E. Coli
Sample Types: Protein
Applications: Bioassay -
Identification of a novel K311 ubiquitination site critical for androgen receptor transcriptional activity
Nucleic Acids Res., 2016;0(0):.
Applications: Bioassay -
The adenovirus E4-ORF3 protein functions as a SUMO E3 ligase for TIF-1? sumoylation and poly-SUMO chain elongation
Proc Natl Acad Sci USA, 2016;113(24):6725-30.
Species: Human
Sample Types: Protein
Applications: Bioassay -
A novel cereblon modulator recruits GSPT1 to the CRL4(CRBN) ubiquitin ligase
Authors: Mary E Matyskiela
Nature, 2016;0(0):.
Species: N/A
Sample Types: Protein
Applications: Bioassay -
Role of SUMO activating enzyme in cancer stem cell maintenance and self-renewal
Nat Commun, 2016;7(0):12326.
Species: N/A
Sample Types: Recombinant Protein
Applications: Bioassay -
Cellular inhibitor of apoptosis (cIAP)-mediated ubiquitination of phosphofurin acidic cluster sorting protein 2 (PACS-2) negatively regulates tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) cytotoxicity.
Authors: Guicciardi, Maria Eu, Werneburg, Nathan W, Bronk, Steven F, Franke, Adrian, Yagita, Hideo, Thomas, Gary, Gores, Gregory
PLoS ONE, 2014;9(3):e92124.
Species: N/A
Sample Types: Recombinant Protein
Applications: Bioassay -
A HECT ubiquitin-protein ligase as a novel candidate gene for altered quinine and quinidine responses in Plasmodium falciparum.
Authors: Sanchez C, Liu C, Mayer S, Nurhasanah A, Cyrklaff M, Mu J, Ferdig M, Stein W, Lanzer M
PLoS Genet, 2014;10(5):e1004382.
Species: Parasite - Plasmodium falciparum
Sample Types: Protein
Applications: Bioassay -
The ubiquitin ligase ASB4 promotes trophoblast differentiation through the degradation of ID2.
Authors: Townley-Tilson, W H Davi, Wu, Yaxu, Ferguson, James E, Patterson, Cam
PLoS ONE, 2014;9(2):e89451.
Species: Human
Sample Types: Whole Cells
Applications: Ubiquitination -
Pin1 modulates ERalpha levels in breast cancer through inhibition of phosphorylation-dependent ubiquitination and degradation.
Authors: Rajbhandari P, Schalper K, Solodin N, Ellison-Zelski S, Ping Lu K, Rimm D, Alarid E
Oncogene, 2014;33(11):1438-47.
Species: N/A
Sample Types: Recombinant Protein
Applications: Bioassay -
A novel role for inhibitor of apoptosis (IAP) proteins as regulators of endothelial barrier function by mediating RhoA activation.
Authors: Hornburger M, Mayer B, Leonhardt S, Willer E, Zahler S, Beyerle A, Rajalingam K, Vollmar A, Furst R
FASEB J, 2013;28(4):1938-46.
Species: Human
Sample Types: Recombinant Protein
Applications: Ubiquitination -
C. elegans ring finger protein RNF-113 is involved in interstrand DNA crosslink repair and interacts with a RAD51C homolog.
Authors: Lee, Hyojin, Alpi, Arno F, Park, Mi So, Rose, Ann, Koo, Hyeon-So
PLoS ONE, 2013;8(3):e60071.
Species: Human
Sample Types: Recombinant Protein
Applications: Enzyme Assay -
Characterization of ML-IAP protein stability and physiological role in vivo.
Authors: Varfolomeev, Eugene, Moradi, Elham, Dynek, Jasmin N, Zha, Jiping, Fedorova, Anna V, Deshayes, Kurt, Fairbrother, Wayne J, Newton, Kim, Le Couter, Jennifer, Vucic, Domagoj
Biochem J, 2012;447(3):427-36.
Species: Human
Sample Types: Protein
Applications: Bioassay
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