Recombinant Human UbcH5a/UBE2D1 Protein, CF

  (25 citations)     
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Product Details
Citations (25)
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  • Purity
    >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
  • Activity
    Recombinant Human UbcH5a/UBE2D1 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UbcH5a/UBE2D1 concentration of 0.1-1 μM.
  • Source
    E. coli-derived human UbcH5a/UBE2D1 protein
  • Accession #
  • Predicted Molecular Mass
    17 kDa
Product Datasheets

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E2-616
 
Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
 
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
 
Background: UbcH5a/UBE2D1

Ubiquitin-conjugating Enzyme H5a (UbcH5a), also known as Ubiquitin-conjugating Enzyme E2D 1 (UBE2D1), is a ubiquitously expressed protein that is related to Stimulator of Iron Transport (SFT) (1,2). Human UbcH5a/UBE2D1 has a predicted molecular weight of 17 kDa and shares 89% and 88% amino acid (aa) sequence identity with the related family members UbcH5b and UbcH5c, respectively (3). Human UbcH5a/UBE2D1 shares 100% aa sequence identity with the mouse and rat orthologs. UbcH5a/UBE2D1 has a conserved E2 catalytic core domain that contains an active site cysteine residue, and it interacts with a variety of HECT and RING finger Ubiquitin ligases (E3) to mediate the ubiquitination of specific target proteins (4). UbcH5a/UBE2D1 interacts with the E3, E6-AP, to conjugate Ubiquitin to the tumor suppressor, p53 (1). Additional protein targets of UbcH5a/UBE2D1 include c-Fos, RIP1, and HIF-1 (5,6). Pathologically, UbcH5a/UBE2D1 is implicated in protein degradation during cancer and immune responses (7).

  • References:
    1. Scheffner, M. et al. (1994) Proc. Natl. Acad. Sci. USA 91:8797.
    2. Gehrke, S. et al. (2003) Blood 101:3288.
    3. Jensen, J. et al. (1995) J. Biol. Chem. 270:30408.
    4. Lorick, K. et al. (2005) Methods Enzymol. 398:54.
    5. Stancovski, I. et al. (1995) Mol. Cell Biol. 15:7106.
    6. Dynek, J. et al. (2010) EMBO J. 29:4128.
    7. Vanni, E. et al. (2012) J. Virol. 86:6323.
  • Entrez Gene IDs:
    7321 (Human); 216080 (Mouse); 361831 (Rat)
  • Alternate Names:
    E2(17)KB1; Stimulator of Fe transportUBC4/5 homolog; UBC4/5; UBC5A; UbcH5; UbcH5a; UBCH5AEC 6.3.2.19; UBCH5SFT; UBE2D1; Ubiquitin carrier protein D1; ubiquitin-conjugating enzyme E2 D1; Ubiquitin-conjugating enzyme E2(17)KB 1; Ubiquitin-conjugating enzyme E2-17 kDa 1; ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, yeast); Ubiquitin-protein ligase D1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Regulation of a distinct activated RIPK1 intermediate bridging complex I and complex II in TNF?-mediated apoptosis
    Authors: P Amin, M Florez, A Najafov, H Pan, J Geng, D Ofengeim, SA Dziedzic, H Wang, VJ Barrett, Y Ito, MJ LaVoie, J Yuan
    Proc. Natl. Acad. Sci. U.S.A., 2018;115(26):E5944-E5953.
    Application: Bioassay
  2. PI5P4K? functions in DTX1-mediated Notch signaling
    Authors: L Zheng, SD Conner
    Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
    Species: Human
    Sample Type: Recombinant Protein
    Application: Bioassay
  3. CNPY2 inhibits MYLIP-mediated AR protein degradation in prostate cancer cells
    Authors: S Ito, A Ueno, T Ueda, H Nakagawa, H Taniguchi, N Kayukawa, A Fujihara-I, F Hongo, K Okihara, O Ukimura
    Oncotarget, 2018;9(25):17645-17655.
    Application: Bioassay
  4. Ubiquitin Modification by the E3 Ligase/ADP-Ribosyltransferase Dtx3L/Parp9
    Authors: CS Yang, K Jividen, A Spencer, N Dworak, L Ni, LT Oostdyk, M Chatterjee, B Ku?mider, B Reon, M Parlak, V Gorbunova, T Abbas, E Jeffery, NE Sherman, BM Paschal
    Mol. Cell, 2017;66(4):503-516.e5.
    Application: Bioassay
  5. Internally tagged ubiquitin: a tool to identify linear polyubiquitin-modified proteins by mass spectrometry
    Authors: K Kliza, C Taumer, I Pinzuti, M Franz-Wach, S Kunzelmann, B Stieglitz, B Macek, K Husnjak
    Nat. Methods, 2017;0(0):.
    Species: N/A
    Sample Type: Protein
    Application: Bioassay
  6. Ubiquitin-dependent regulation of Cdc42 by XIAP
    Authors: A Murali, J Shin, H Yurugi, A Krishnan, M Akutsu, A Carpy, B Macek, K Rajalingam
    Cell Death Dis, 2017;8(6):e2900.
    Species: Human
    Sample Type: Purified Proteins
    Application: Bioassay
  7. Thymine DNA glycosylase modulates DNA damage response and gene expression by base excision repair-dependent and independent mechanisms
    Authors: T Nakamura, K Murakami, H Tada, Y Uehara, J Nogami, K Maehara, Y Ohkawa, H Saitoh, H Nishitani, T Ono, R Nishi, M Yokoi, W Sakai, K Sugasawa
    Genes Cells, 2017;0(0):.
    Application: Bioassay
  8. Keap1/Cullin3 Modulates p62/SQSTM1 Activity via UBA Domain Ubiquitination
    Authors: Y Lee, TF Chou, SK Pittman, AL Keith, B Razani, CC Weihl
    Cell Rep, 2017;19(1):188-202.
    Species: Mouse
    Sample Type: Protein
    Application: Bioassay
  9. Ubiquitin Modification by the E3 Ligase/ADP-Ribosyltransferase Dtx3L/Parp9
    Authors: CS Yang, K Jividen, A Spencer, N Dworak, L Ni, LT Oostdyk, M Chatterjee, B Ku?mider, B Reon, M Parlak, V Gorbunova, T Abbas, E Jeffery, NE Sherman, BM Paschal
    Mol. Cell, 2017;66(4):503-516.e5.
    Species: Human
    Sample Type: Protein
    Application: Bioassay
  10. Keap1/Cullin3 Modulates p62/SQSTM1 Activity via UBA Domain Ubiquitination
    Authors: Y Lee, TF Chou, SK Pittman, AL Keith, B Razani, CC Weihl
    Cell Rep, 2017;19(1):188-202.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  11. DNA damage and S phase-dependent E2F1 stabilization requires the cIAP1 E3-ubiquitin ligase and is associated with K63-poly-ubiquitination on lysine 161/164 residues
    Authors: V Glorian, J Allègre, J Berthelet, B Dumetier, PM Boutanquoi, N Droin, C Kayaci, J Cartier, S Gemble, G Marcion, D Gonzalez, R Boidot, C Garrido, O Michaud, E Solary, L Dubrez
    Cell Death Dis, 2017;8(5):e2816.
    Species: Bacteria - E. Coli
    Sample Type: Protein
    Application: Bioassay
  12. DNA damage and S phase-dependent E2F1 stabilization requires the cIAP1 E3-ubiquitin ligase and is associated with K63-poly-ubiquitination on lysine 161/164 residues
    Authors: V Glorian, J Allègre, J Berthelet, B Dumetier, PM Boutanquoi, N Droin, C Kayaci, J Cartier, S Gemble, G Marcion, D Gonzalez, R Boidot, C Garrido, O Michaud, E Solary, L Dubrez
    Cell Death Dis, 2017;8(5):e2816.
    Species: Human
    Sample Type: Recombinant Protein
    Application: Bioassay
  13. Ubiquitin-dependent regulation of Cdc42 by XIAP
    Authors: A Murali, J Shin, H Yurugi, A Krishnan, M Akutsu, A Carpy, B Macek, K Rajalingam
    Cell Death Dis, 2017;8(6):e2900.
    Species: N/A
    Sample Type: Proteins
    Application: Bioassay
  14. pSILAC mass spectrometry reveals ZFP91 as IMiD-dependent substrate of the CRL4(CRBN) ubiquitin ligase
    Authors: J An, CM Ponthier, R Sack, J Seebacher, MB Stadler, KA Donovan, ES Fischer
    Nat Commun, 2017;8(0):15398.
    Species: N/A
    Sample Type: Proteins
    Application: Bioassay
  15. Role of SUMO activating enzyme in cancer stem cell maintenance and self-renewal
    Nat Commun, 2016;7(0):12326.
    Species: N/A
    Sample Type: Recombinant Protein
    Application: Bioassay
  16. The adenovirus E4-ORF3 protein functions as a SUMO E3 ligase for TIF-1? sumoylation and poly-SUMO chain elongation
    Proc Natl Acad Sci USA, 2016;113(24):6725-30.
    Species: Human
    Sample Type: Protein
    Application: Bioassay
  17. A novel cereblon modulator recruits GSPT1 to the CRL4(CRBN) ubiquitin ligase
    Authors: Mary E Matyskiela
    Nature, 2016;0(0):.
    Species: N/A
    Sample Type: Protein
    Application: Bioassay
  18. Identification of a novel K311 ubiquitination site critical for androgen receptor transcriptional activity
    Nucleic Acids Res., 2016;0(0):.
    Application: Bioassay
  19. A HECT ubiquitin-protein ligase as a novel candidate gene for altered quinine and quinidine responses in Plasmodium falciparum.
    Authors: Sanchez C, Liu C, Mayer S, Nurhasanah A, Cyrklaff M, Mu J, Ferdig M, Stein W, Lanzer M
    PLoS Genet, 2014;10(5):e1004382.
    Species: Plasmodium falciparum
    Sample Type: Protein
    Application: Bioassay
  20. The ubiquitin ligase ASB4 promotes trophoblast differentiation through the degradation of ID2.
    Authors: Townley-Tilson, W H Davi, Wu, Yaxu, Ferguson, James E, Patterson, Cam
    PLoS ONE, 2014;9(2):e89451.
    Species: Human
    Sample Type: Whole Cells
    Application: Ubiquitination
  21. Pin1 modulates ERalpha levels in breast cancer through inhibition of phosphorylation-dependent ubiquitination and degradation.
    Authors: Rajbhandari P, Schalper K, Solodin N, Ellison-Zelski S, Ping Lu K, Rimm D, Alarid E
    Oncogene, 2014;33(11):1438-47.
    Species: N/A
    Sample Type: Recombinant Protein
    Application: Bioassay
  22. Cellular inhibitor of apoptosis (cIAP)-mediated ubiquitination of phosphofurin acidic cluster sorting protein 2 (PACS-2) negatively regulates tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) cytotoxicity.
    Authors: Guicciardi, Maria Eu, Werneburg, Nathan W, Bronk, Steven F, Franke, Adrian, Yagita, Hideo, Thomas, Gary, Gores, Gregory
    PLoS ONE, 2014;9(3):e92124.
    Species: N/A
    Sample Type: Recombinant Protein
    Application: Bioassay
  23. C. elegans ring finger protein RNF-113 is involved in interstrand DNA crosslink repair and interacts with a RAD51C homolog.
    Authors: Lee, Hyojin, Alpi, Arno F, Park, Mi So, Rose, Ann, Koo, Hyeon-So
    PLoS ONE, 2013;8(3):e60071.
    Species: Human
    Sample Type: Recombinant Protein
    Application: EnzAct
  24. A novel role for inhibitor of apoptosis (IAP) proteins as regulators of endothelial barrier function by mediating RhoA activation.
    Authors: Hornburger M, Mayer B, Leonhardt S, Willer E, Zahler S, Beyerle A, Rajalingam K, Vollmar A, Furst R
    FASEB J, 2013;28(4):1938-46.
    Species: Human
    Sample Type: Recombinant Protein
    Application: Ubiquination
  25. Characterization of ML-IAP protein stability and physiological role in vivo.
    Authors: Varfolomeev, Eugene, Moradi, Elham, Dynek, Jasmin N, Zha, Jiping, Fedorova, Anna V, Deshayes, Kurt, Fairbrother, Wayne J, Newton, Kim, Le Couter, Jennifer, Vucic, Domagoj
    Biochem J, 2012;447(3):427-36.
    Species: Human
    Sample Type: Protein
    Application: Bioassay
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