Recombinant Human ULBP-2 Fc Chimera Avi-tag Protein, CF

Biotinylated
Catalog # Availability Size / Price Qty
AVI1298-050
Recombinant Human ULBP-2 Fc Chimera Avi-tag Protein Binding Activity.
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Recombinant Human ULBP-2 Fc Chimera Avi-tag Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human NKG2D/CD314 Fc Chimera  (Catalog # 1299-NK) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human ULBP-2 Fc Chimera Avi-tag (Catalog # AVI1298) binds with an ED50 of 0.500-8.00 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human ULBP-2 protein
Human ULBP-2
(Gly26-Ser217)
Accession # Q9BZM5.1
IEGRMDHuman IgG1
(Pro100-Lys330)
Avi-tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Gly26
Structure / Form
Disulfide-linked homodimer
Biotinylated via Avi-tag
Predicted Molecular Mass
50 kDa
SDS-PAGE
60-70 kDa, under reducing conditions.

Product Datasheets

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AVI1298

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI1298

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity View Larger

When Recombinant Human NKG2D/CD314 Fc Chimera Protein (1299-NK) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human ULBP‑2 Fc Chimera Avi-tag Protein (Catalog # AVI1298) binds with an ED50 of 0.500-8.00 ng/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Human ULBP-2 Fc Chimera Avi-tag Protein (Catalog # AVI1298) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-70 kDa and 120-140 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

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Background: ULBP-2

ULBP-2 is a member of a family of cell-surface proteins that function as ligands for human NKG2D. ULBP-2 has also been described under the names RaeT1H (retinoic acid early transcript), NKG2DL2, and ALCAN-alpha. The name ULBP-2 derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The gene for ULBP-2 resides in a cluster of ten related genes, six of which encode potentially functional glycoproteins. Amino acid sequence identity within this family ranges from 30-60%. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2-microglobulin. Some family members, including ULBP-2, are anchored to the membrane via a GPI-linkage, whereas others have transmembrane domains. ULBP-2 and several other family members are known to bind to human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. The ULBPs are expressed on some tumor cells and have been implicated in tumor surveillance. Our Avi-tag Biotinylated Recombinant Human ULBP-2 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide.  Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References
  1. Cosman, D. et al. (2001) Immunity 14:123.
  2. Kubin, M. et al. (2001) Eur. J. Immunol. 31:1428.
  3. Sutherland, C. et al. (2002) J. Immunol. 168:671.
  4. Steinle, A. et al. (2001) Immunogenetics 53:279.
  5. Sutherland, C. et al. (2001) Immunol. Rev. 181:185.
  6. Pende, D. et al. (2002) Cancer Res. 62:6178.
  7. Radosavljevic, M. et al. (2002) Genomics 79:114.
  8. NKG2D and its Ligands (2002) www.rndsystems.com.
Long Name
UL16 Binding Protein-2
Entrez Gene IDs
80328 (Human)
Alternate Names
ALCAN-alpha; N2DL2; N2DL-2; NKG2D ligand 2; NKG2DL2; RAET1H; RAET1HNKG2DL2; retinoic acid early transcript 1 H; Retinoic acid early transcript 1H; UL16 binding protein 2; UL16-binding protein 2; ULBP2; ULBP-2

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