Recombinant Human Uteroglobin Protein, CF

Catalog # Availability Size / Price Qty
4218-UT-050
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (4)

Recombinant Human Uteroglobin Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of the A549 human lung carcinoma cells. The ED50 for this effect is 1‑5 µg/mL.
Source
E. coli-derived human Uteroglobin/SCGB1A1 protein
Glu22-Asn91, with an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Met
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
8 kDa (monomer)

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4218-UT

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Uteroglobin/SCGB1A1

Uteroglobin, also called Clara cell secretory, phospholipid binding, 10 kDa or 16 kDa protein (CCSP, CCPBP, CC10 or CC16, respectively) is a small, non-glycosylated secreted protein of the secretoglobin superfamily, (designated 1A, member 1) (1 - 3). Its name is derived from its very high expression in the pre-implantation uterus. It is produced by the non-ciliated, non-mucous secretory cells that predominate in lung bronchioles (Clara cells), and other non-ciliated epithelia that communicate with the external environment (1 - 3). Expression is induced by steroid hormones such as estrogen, and enhanced by the non-steroid hormone prolactin (1). Uteroglobin is found in blood, urine and other body fluids (1). Human Uteroglobin cDNA encodes a 21 amino acid (aa) signal sequence and a 70 aa mature protein. It shares 53 - 56% aa identity with mouse, rat, bovine, canine, equine or rabbit Uteroglobin, and is active in mice (4). The mature protein forms a disulfide-linked head-to-tail homodimer of 16 kDa (2, 5). This homodimer is thought to form a binding pocket that binds hydrophobic ligands such as phospholipids, progesterone and retinols (5). Sequestering of prostaglandins and leukotrienes is anti-inflammatory, while sequestering of carcinogens such as polychlorinated bisphenols is anti-tumorigenic (6 - 8). Other immunoregulatory activities of Uteroglobin include cell migration inhibition (by binding the chemotaxis-related formyl peptide receptor FPR2 on dendritic cells), and the inhibition of T cell differentiation to a Th2 phenotype (9). A single nucleotide polymorphism of Uteroglobin, A38G, confers increased risk of asthma (10). Transglutaminase can crosslink Uteroglobin, either to itself or to other proteins such as the adhesion molecule fibronectin (3, 11). Binding of fibronectin to Uteroglobin in the kidney is thought to protect against nephropathy, while binding of the lipocalin-1 receptor has been reported to suppress cancer cell motility and invasion (12, 13).

References
  1. Mukherjee, A.B. et al. (2007) Endocr. Rev. 28:707.
  2. Singh, G. et al. (1988) Biochim. Biophys. Acta 950:329.
  3. Mantile, G. et al. (1993) J. Biol. Chem. 268:20343.
  4. Wang, S-Z. et al. (2003) J. Immunol. 171:1051.
  5. Umland, T.C. et al. (1994) Nat. Struct. Biol. 1:538.
  6. Mandal, A.K. et al. (2004) J. Exp. Med. 199:1317.
  7. Watson, T.M. et al. (2001) Am. J. Physiol. Lung Cell Mol. Physiol. 281:L1523.
  8. Yang, Y. et al. (2004) J. Biol. Chem. 279:29336.
  9. Ray, R. et al. (2006) FEBS Lett. 580:6022.
  10. Martin, A.C. et al. (2006) Am. J. Respir. Crit. Care Med. 173:617.
  11. Antico, G. et al. (2006) J. Cell Physiol. 207:553.
  12. Zheng, F. et al. (1999) Nat. Med. 5:1018.
  13. Zhang, Z. et al. (2006) Gene 369:66.
Entrez Gene IDs
7356 (Human); 22287 (Mouse); 25575 (Rat)
Alternate Names
Blastokinin; CC10; CC10Urine protein 1; CC16; CCPBP; CCSP; CCSPCCPBP; Clara cell phospholipid-binding protein; Clara cells 10 kDa secretory protein; Clara; PCB-BP; SCGB1A1; Secretoglobin family 1A member 1; secretoglobin, family 1A, member 1 (uteroglobin); UGB; UGBUP-1; UP1; Urinary protein 1; Uteroglobin

Citation for Recombinant Human Uteroglobin Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The expression of osteopontin and its association with Clara cell 10 kDa protein in allergic rhinitis.
    Authors: Liu Y, Lu X, Yu HJ, Hua XY, Cui YH, Huang SK, Liu Z
    Clin. Exp. Allergy, 2010;40(11):1632-41.

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Reviews for Recombinant Human Uteroglobin Protein, CF

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Recombinant Human Uteroglobin Protein, CF
By Anonymous on 11/10/2019
Application: In vitro bioactivity in cell culture

Recombinant Human Uteroglobin Protein, CF
By Anonymous on 05/23/2019
Application: Binding assay/Protein-protein interaction

Recombinant Human Uteroglobin Protein, CF
By Anonymous on 08/20/2018
Application: Binding assay/Protein-protein interaction

Recombinant Human Uteroglobin Protein, CF
By Anonymous on 12/01/2017
Application: Immunoassay Standard