Uteroglobin, also called Clara cell secretory, phospholipid binding, 10 kDa or 16 kDa protein (CCSP, CCPBP, CC10 or CC16, respectively) is a small, non-glycosylated secreted protein of the secretoglobin superfamily, (designated 1A, member 1) (1 - 3). Its name is derived from its very high expression in the pre-implantation uterus. It is produced by the non-ciliated, non-mucous secretory cells that predominate in lung bronchioles (Clara cells), and other non-ciliated epithelia that communicate with the external environment (1 - 3). Expression is induced by steroid hormones such as estrogen, and enhanced by the non-steroid hormone prolactin (1). Uteroglobin is found in blood, urine and other body fluids (1). Human Uteroglobin cDNA encodes a 21 amino acid (aa) signal sequence and a 70 aa mature protein. It shares 53 - 56% aa identity with mouse, rat, bovine, canine, equine or rabbit Uteroglobin, and is active in mice (4). The mature protein forms a disulfide-linked head-to-tail homodimer of 16 kDa (2, 5). This homodimer is thought to form a binding pocket that binds hydrophobic ligands such as phospholipids, progesterone and retinols (5). Sequestering of prostaglandins and leukotrienes is anti-inflammatory, while sequestering of carcinogens such as polychlorinated bisphenols is anti-tumorigenic (6 - 8). Other immunoregulatory activities of Uteroglobin include cell migration inhibition (by binding the chemotaxis-related formyl peptide receptor FPR2 on dendritic cells), and the inhibition of T cell differentiation to a Th2 phenotype (9). A single nucleotide polymorphism of Uteroglobin, A38G, confers increased risk of asthma (10). Transglutaminase can crosslink Uteroglobin, either to itself or to other proteins such as the adhesion molecule fibronectin (3, 11). Binding of fibronectin to Uteroglobin in the kidney is thought to protect against nephropathy, while binding of the lipocalin-1 receptor has been reported to suppress cancer cell motility and invasion (12, 13).
Recombinant Human Uteroglobin Protein, CF
R&D Systems | Catalog # 4218-UT
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Key Product Details
- R&D Systems E. coli-derived Recombinant Human Uteroglobin Protein (4218-UT)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
E. coli
Accession Number
Structure / Form
Disulfide-linked homodimer
Applications
Bioactivity
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Product Specifications
Source
E. coli-derived human Uteroglobin/SCGB1A1 protein
Glu22-Asn91, with an N-terminal Met
Glu22-Asn91, with an N-terminal Met
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
8 kDa (monomer)
Activity
Measured by the ability of the immobilized protein to support the adhesion of the A549 human lung carcinoma cells.
The ED50 for this effect is 1‑5 µg/mL.
The ED50 for this effect is 1‑5 µg/mL.
Reviewed Applications
Read 4 reviews rated 5 using 4218-UT in the following applications:
Formulation, Preparation, and Storage
4218-UT
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS.
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| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Calculators
Background: Uteroglobin/SCGB1A1
References
- Mukherjee, A.B. et al. (2007) Endocr. Rev. 28:707.
- Singh, G. et al. (1988) Biochim. Biophys. Acta 950:329.
- Mantile, G. et al. (1993) J. Biol. Chem. 268:20343.
- Wang, S-Z. et al. (2003) J. Immunol. 171:1051.
- Umland, T.C. et al. (1994) Nat. Struct. Biol. 1:538.
- Mandal, A.K. et al. (2004) J. Exp. Med. 199:1317.
- Watson, T.M. et al. (2001) Am. J. Physiol. Lung Cell Mol. Physiol. 281:L1523.
- Yang, Y. et al. (2004) J. Biol. Chem. 279:29336.
- Ray, R. et al. (2006) FEBS Lett. 580:6022.
- Martin, A.C. et al. (2006) Am. J. Respir. Crit. Care Med. 173:617.
- Antico, G. et al. (2006) J. Cell Physiol. 207:553.
- Zheng, F. et al. (1999) Nat. Med. 5:1018.
- Zhang, Z. et al. (2006) Gene 369:66.
Alternate Names
Blastokinin, CC10, CC16, CCPBP, CCSP, Clara, PCB-BP, SCGB1A1, UGB
Gene Symbol
SCGB1A1
UniProt
Additional Uteroglobin/SCGB1A1 Products
Product Documents for Recombinant Human Uteroglobin Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human Uteroglobin Protein, CF
For research use only
Related Research Areas
Citations for Recombinant Human Uteroglobin Protein, CF
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Customer Reviews for Recombinant Human Uteroglobin Protein, CF (4)
5 out of 5
4 Customer Ratings
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 11/10/2019
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 05/23/2019
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 08/20/2018
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Application: Immunoassay StandardVerified Customer | Posted 12/01/2017
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