Recombinant Human VEGF-B 167 Protein, CF

Newer Version Available: 751-VEB
NEW

Discontinued Product

751-VE has been discontinued and is replaced by 751-VEB.

R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Citations (7)
FAQs
Reviews (1)

Recombinant Human VEGF-B 167 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized recombinant rat Neuropilin-1 Fc Chimera at 4 µg/mL (100 µL/well) can bind
recombinant human VEGF-B167 with a linear range of 0.3-20 ng/mL.
Source
E. coli-derived human VEGF-B protein
Pro22-Arg188
Accession #
N-terminal Sequence
Analysis
Pro22
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
19 kDa (monomer)

Product Datasheets

You must select a language.

x

751-VE

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

751-VE

Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitution in 4 mM HCl at 500 ug/mL.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: VEGF-B

Vascular endothelial growth factor B (VEGF-B), also known as vascular endothelial growth factor-related factor (VRF), is a member of the VEGF family of growth factors that share structural and functional similarity (1, 2). Five mammalian members, including VEGF-A, -B, -C, -D and PlGF, have been identified. VEGF family members are disulfide-linked dimeric proteins that are important regulators of physiological and pathological vasculogenesis, angiogenesis and lymphangiogenesis. VEGF-B is expressed in most tissues, especially in heart, skeletal muscle and pancreas. In many tissues, VEGF-B is co-expressed and can heterodimerize with VEGF (3). By alternative splicing, two isoforms of mature VEGF-B containing 167 or 186 amino acid (aa) residues exist (3, 4). The two VEGF-B isoforms have identical amino-terminal cysteine-knot VEGF homology domains but the carboxyl end of VEGF-B167 differs from that of VEGF-B186 by the presence of a highly basic cysteine-rich heparin binding domain. Whereas VEGF-B186 is a secreted diffusible protein, VEGF-B167 is sequestered into the cell matrix after secretion. Both VEGF-B isoforms bind VEGF receptor 1 (VEGF R1), but not VEGF R2 or VEGF R3 (5). On endothelial cells, ligation of VEGF R1 by VEGF-B has been shown to regulate the expression and activity of urokinase type plasminogen activator and plasminogen activator inhibitor 1. VEGF-B167 and a proteolytically processed form of VEGF-B186 (VEGF-B127) also bind neuropilin-1 (NP-1), a type I transmembrane receptor for semaphorins/collapsins, ligands involved in neuron guidance (6). Besides VEGF-B, NP‑1 has been shown to bind PLGF-2, VEGF165 and VEGF R1 (6, 7). The many interactions of NP-1 with VEGF ligands and receptor suggests that NP-1 may function as a regulator of angiogenesis (7).

References
  1. Li, X. and U. Eriksson (2001) Int. J. Biochem Cell Biol. 33:421.
  2. Olofsson, B. et al. (1999) Curr. Opin. Biotechnol. 10:528.
  3. Olofsson, B. et al. (1996) Proc. Nat. Acad. Sci. USA 93:2576.
  4. Grimmond, S. et al. (1996) Benome Res. 6:124.
  5. Olofsson, B. et al. (1998) Proc. Nat. Acad. Sci. USA 95:11709.
  6. Makinen, T. et al. (1999) J. Biol. Chem. 274:21217.
  7. Fuh, G. et al. (2000) J. Biol. Chem. 275:26690.
Long Name
Vascular Endothelial Growth Factor B
Entrez Gene IDs
7423 (Human); 22339 (Mouse)
Alternate Names
vascular endothelial growth factor B; VEGFB; VEGF-B; VEGF-related factor; VRFVEGFL

Citations for Recombinant Human VEGF-B 167 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
Filter your results:

Filter by:

  1. Systemic VEGF-A neutralization ameliorates diet-induced metabolic dysfunction.
    Authors: Wu L, Meoli C, Mangiafico S, Fazakerley D, Cogger V, Mohamad M, Pant H, Kang M, Powter E, Burchfield J, Xirouchaki C, Mikolaizak A, Stockli J, Kolumam G, van Bruggen N, Gamble J, Le Couteur D, Cooney G, Andrikopoulos S, James D
    Diabetes, 2014;63(8):2656-67.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Multiple receptor tyrosine kinases regulate HIF-1alpha and HIF-2alpha in normoxia and hypoxia in neuroblastoma: implications for antiangiogenic mechanisms of multikinase inhibitors.
    Authors: Nilsson MB, Zage PE, Zeng L, Xu L, Cascone T, Wu HK, Saigal B, Zweidler-McKay PA, Heymach JV
    Oncogene, 2010;29(20):2938-49.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. The 12th-14th type III repeats of fibronectin function as a highly promiscuous growth factor-binding domain.
    Authors: Martino MM, Hubbell JA
    FASEB J., 2010;24(12):4711-21.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  4. Placental growth factor neutralising antibodies give limited anti-angiogenic effects in an in vitro organotypic angiogenesis model.
    Authors: Brave SR, Eberlein C, Shibuya M, Wedge SR, Barry ST
    Angiogenesis, 2010;13(4):337-47.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Modulation of angiogenesis by a tetrameric tripeptide that antagonizes vascular endothelial growth factor receptor 1.
    Authors: Ponticelli S, Marasco D, Tarallo V, Albuquerque RJ, Mitola S, Takeda A, Stassen JM, Presta M, Ambati J, Ruvo M, De Falco S
    J. Biol. Chem., 2008;283(49):34250-9.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  6. Ligand-induced internalization selects use of common receptor neuropilin-1 by VEGF165 and semaphorin3A.
    Authors: Narazaki M, Tosato G
    Blood, 2006;107(10):3892-901.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  7. Vascular endothelial growth factor-B promotes in vivo angiogenesis.
    Authors: Silvestre JS, Tamarat R, Ebrahimian TG, Le-Roux A, Clergue M, Emmanuel F, Duriez M, Schwartz B, Branellec D, Levy BI
    Circ. Res., 2003;93(2):114-23.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human VEGF-B 167 Protein, CF

Average Rating: 3 (Based on 1 Review)

5 Star
0%
4 Star
0%
3 Star
100%
2 Star
0%
1 Star
0%

Have you used Recombinant Human VEGF-B 167 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Recombinant Human VEGF-B 167 Protein, CF
By Anonymous on 02/07/2018
Application: SDS-PAGE Control