Recombinant Human VEGFR3/Flt-4 His-tag Avi-tag Protein, CF

R&D Systems | Catalog # AVI10384

Biotinylated
R&D Systems
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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human VEGFR3/Flt-4 His-tag Avi-tag Protein (AVI10384)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

P35916.3

Structure / Form

Monomer, biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity
View all VEGFR3/Flt-4 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human VEGFR3/Flt-4 protein
Human VEGFR3/Flt-4
(Tyr25-Glu775)
Accession # P35916.3		 HHHHHH Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr25

Predicted Molecular Mass

87 kDa

SDS-PAGE

115-140 kDa, 72-89 kDa and 60-70 kDa

Activity

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay.

Measured by its binding ability in a functional ELISA.
When Recombinant Human VEGF-C (Catalog # 9199-VC) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human VEGFR3/Flt-4 His-tag Avi-tag (Catalog # AVI10384) binds with an ED50 of 15-90 ng/mL.

Reviewed Applications

Read 1 review rated 4 using AVI10384 in the following applications:

Scientific Data Images for Recombinant Human VEGFR3/Flt-4 His-tag Avi-tag Protein, CF

Recombinant Human VEGFR3/Flt-4 His-tag Avi-tag Protein Binding Activity

Biotinylated Recombinant Human VEGFR3/Flt-4 His-tag Avi-tag Protein Binding Activity

When Recombinant Human VEGF-C (Catalog # 9199-VC) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human VEGFR3/Flt-4 His-tag Avi-tag (Catalog # AVI10384) binds with an ED50 of 15-90 ng/mL.

Formulation, Preparation, and Storage

AVI10384
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VEGFR3/Flt-4

VEGFR2 (KDR/Flk-1), VEGFR1 (Flt‑1) and VEGFR3 (Flt‑4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. VEGFR3 cDNA encodes a 1298 amino acid (aa) residue precursor protein with a 24 aa residue signal peptide. Mature VEGFR3 is composed of a 751 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 482 aa residue cytoplasmic domain. Both VEGF-C and VEGF-D have been shown to bind and activate VEGFR3 (Flt‑4). VEGFR3 is widely expressed in the early embryo but becomes restricted to lymphatic endothelia at later stages of development. It is likely that VEGFR3 may be important for lymph angiogenesis.

References

  1. Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.

Long Name

Vascular Endothelial Growth Factor Receptor 3

Alternate Names

Flt-4, FLT4, VEGF R3

Entrez Gene IDs

2324 (Human); 14257 (Mouse)

Gene Symbol

FLT4

UniProt

P35916.3

Additional VEGFR3/Flt-4 Products

Product Documents for Recombinant Human VEGFR3/Flt-4 His-tag Avi-tag Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human VEGFR3/Flt-4 His-tag Avi-tag Protein, CF

For research use only

Customer Reviews for Recombinant Human VEGFR3/Flt-4 His-tag Avi-tag Protein, CF (1)

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    Recombinant Human VEGFR3/Flt-4 His-tag Avi-tag Protein, CF AVI10384

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