Recombinant Human VG5Q Protein, CF

Catalog # Availability Size / Price Qty
3048-VG-025

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Recombinant Human VG5Q Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized rhVG5Q at 1 µg/mL (100 µL/well) can bind rhTWEAK with a linear range of 0.8-50 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human VG5Q protein
Ala2-Glu714, with an N-terminal 9-His tag
Accession #
N-terminal Sequence
Analysis
His
Predicted Molecular Mass
82.0 kDa
SDS-PAGE
120-140 kDa, reducing conditions

Product Datasheets

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3048-VG

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3048-VG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: VG5Q

VG5Q, also known as AGGF1, was identified by its association with Klippel-Trenaunay syndrome (KTS), a congenital vascular morphogenesis disorder (1 - 3). Two genetic alterations found in KTS lead to aberrant expression or function of VG5Q: a chromosomal translocation that falls within the VG5Q promoter and a glutamate to lysine point mutation at position 133 (1). The human VG5Q cDNA encodes a 714 amino acid (aa) precursor with a coiled coil domain, an FHA domain, a G-patch domain, and an OCRE domain. FHA domains bind phosphorylated threonine and tyrosine residues (4, 5). G-patch and OCRE motif-containing proteins associate with RNA and are implicated in RNA metabolism (6, 7). Two splice variants (109 aa and 176 aa) of human VG5Q have C-terminal deletions and contain alternate sequences at their C-termini. The FHA, G-patch, and OCRE domains are absent in both of these splice variants. Human VG5Q shares 78% and 89% aa identity with mouse and canine VG5Q, respectively. Bovine VG5Q has a C-terminal truncation following position 294 but shares 85% aa identity with the corresponding region of human VG5Q. VG5Q is expressed by vascular endothelial cells in many tissues (1). It appears to be secreted and promotes endothelial cell proliferation following interactions with endothelial cell surfaces (1). VG5Q also directly interacts with TWEAK (1), a TNF superfamily ligand with angiogenic properties (8). The FHA, G-patch, and OCRE domains of VG5Q suggest additional intracellular functions.

References
  1. Tian, X-L. et al. (2004) Nature 427:640.
  2. Timur, A.A et al. (2005) Cell. Mol. Life Sci. 62:1434.
  3. Lambrechts, D. and P. Carmeliet (2004) Nature 427:592.
  4. Durocher, D. and S.P. Jackson (2002) FEBS Lett. 513:58.
  5. Wang, P. et al. (2000) J. Mol. Biol. 302:927.
  6. Aravind, L. and E.V. Koonin (1999) Trends Biochem. Sci. 24:342.
  7. Callebaut, I. and J.P. Mornon (2005) Bioinformatics 21:699.
  8. Wiley, S.R. and J.A. Winkles (2003) Cytokine Growth Factor Rev. 14:241.
Long Name
Vasculogenesis Gene on 5q/Angiogenic Factor with G Patch and FHA Domains 1
Entrez Gene IDs
55109 (Human)
Alternate Names
AGGF1; Angiogenic factor VG5Q; angiogenic factor with G patch and FHA domains 1; FLJ10283hVG5Q; GPATC7G patch domain-containing protein 7; GPATCH7HUS84971; HSU84971; VG5Q; VG5QVasculogenesis gene on 5q protein

Citation for Recombinant Human VG5Q Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Aggf1 attenuates neuroinflammation and BBB disruption via PI3K/Akt/NF-?B pathway after subarachnoid hemorrhage in rats
    Authors: Q Zhu, B Enkhjargal, L Huang, T Zhang, C Sun, Z Xie, P Wu, J Mo, J Tang, Z Xie, JH Zhang
    J Neuroinflammation, 2018-06-09;15(1):178.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo

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