Recombinant Human Vitamin D BP His-tag Protein, CF Summary
Leu17-Leu474 (K436T), with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||
2 μg/lane of Recombinant Vitamin D BP was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 57-63 kDa and 45-50 kDa, respectively.
Background: Vitamin D BP
Vitamin D BP (Vitamin D binding protein), also known as group-specific component and GC-globulin, is a 58 kDa, monomeric glycoprotein member of the ALB/AFP/VDB family. It was initially discovered as a major liver-derived polymorphic protein and was called group-specific component or GC in 1959 (1). Mature human Vitamin D BP is 458 amino acids (aa) in length. It contains three albumin-type domains that are accompanied by 14 intramolecular disulfide bonds (2). Mature human Vitamin D BP is 77% aa identical to mouse Vitamin D BP. Vitamin D BP is able to bind to various forms of Vitamin D including vitamin D2, the 25‑hydroxylated forms and the active hormonal product, 1,25-dihydroxyvitamin D (Calcitriol). The major proportion of Vitamin D in blood is bound to Vitamin D BP, which transports vitamin D metabolites between skin, liver and kidney and then on to various target tissues (3). Vitamin D BP is implemented as a cause for multiple sclerosis (4). There is a strong suggestion that vitamin D and Vitamin D BP gene polymorphism are involved in occurrence of type 2 diabetes mellitus (5). Vitamin D BP has been associated with inflammatory process, stimulation of chemotaxis by phagocytic neutrophils and the activation and stimulation of phagocytic function by macrophages (6, 7).
- Hirschfeld, J. (1959) Acta. Pathol. Microbiol. Scand. 47:160.
- Schoentgen, F. et al. (1986) Biochim. Biophys. Acta. 871:189.
- Verboven, C. et al. (2002) Nat. Struct. Biol. 9:131.
- Langer-Gould, A. et al. (2018) Nutrients. 10:E184.
- Rahman, M.M. et al. (2017) Gene 636:42.
- Binder, R. et al. (1999) Mol. Immunol. 36:885.
- Kew, R.R. et al. (1995) J Immunol. 155:5369.
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