Recombinant Human Vitamin D BP His-tag Protein, CF

Catalog # Availability Size / Price Qty
3778-DB-050
Recombinant Human Vitamin D BP His-tag Protein, CF SDS-PAGE
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Recombinant Human Vitamin D BP His-tag Protein, CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Vitamin D3-BSA Conjugate is immobilized at 2 μg/mL, 100 μL/well, Recombinant Human Vitamin D BP binds with an ED50 of 2‑10 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human Vitamin D BP protein
Leu17-Leu474 (K436T), with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Leu17
Predicted Molecular Mass
52 kDa
SDS-PAGE
57-63 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3778-DB

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Image

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Vitamin D BP was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 57-63 kDa and 45-50 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Vitamin D BP

Vitamin D BP (Vitamin D binding protein), also known as group-specific component and GC-globulin, is a 58 kDa, monomeric glycoprotein member of the ALB/AFP/VDB family. It was initially discovered as a major liver-derived polymorphic protein and was called group-specific component or GC in 1959 (1). Mature human Vitamin D BP is 458 amino acids (aa) in length. It contains three albumin-type domains that are accompanied by 14 intramolecular disulfide bonds (2). Mature human Vitamin D BP  is 77% aa identical to mouse Vitamin D BP. Vitamin D BP is able to bind to various forms of Vitamin D including vitamin D2, the 25‑hydroxylated forms and the active hormonal product, 1,25-dihydroxyvitamin D (Calcitriol). The major proportion of Vitamin D in blood is bound to Vitamin D BP, which transports vitamin D metabolites between skin, liver and kidney and then on to various target tissues (3). Vitamin D BP is implemented as a cause for multiple sclerosis (4). There is a strong suggestion that vitamin D and Vitamin D BP  gene polymorphism are involved in occurrence of type 2 diabetes mellitus (5). Vitamin D BP  has been associated with inflammatory process, stimulation of chemotaxis by phagocytic neutrophils and the activation and stimulation of phagocytic function by macrophages (6, 7).

References
  1. Hirschfeld, J. (1959) Acta. Pathol. Microbiol. Scand. 47:160.
  2. Schoentgen, F. et al. (1986) Biochim. Biophys. Acta. 871:189.
  3. Verboven, C. et al. (2002) Nat. Struct. Biol. 9:131.
  4. Langer-Gould, A. et al. (2018) Nutrients. 10:E184.
  5. Rahman, M.M. et al. (2017) Gene 636:42.
  6. Binder, R. et al. (1999) Mol. Immunol. 36:885.
  7. Kew, R.R. et al. (1995) J Immunol. 155:5369.
Long Name
Vitamin D Binding Protein
Entrez Gene IDs
2638 (Human); 14473 (Mouse); 24384 (Rat)
Alternate Names
DBP; DBP/GC; DBP-MAF; DBPVDBG; EC 6.3.1.5; Gc; gc-globulin; Gc-MAF; GRD3; group-specific component (vitamin D binding protein); Group-specific component; hDBP; VDB; VDBG; VDBP; Vitamin D BP; vitamin D-binding alpha-globulin; vitamin D-binding protein

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