Recombinant Mouse Adiponectin/Acrp30 Protein, CF

Catalog # Availability Size / Price Qty
5095-AC-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (6)
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Recombinant Mouse Adiponectin/Acrp30 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce TIMP-1 secretion by mouse macrophages. Kumada, M. et al. (2004) Circulation 109:2046. The ED50 for this effect is 5-20 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Adiponectin/Acrp30 protein
Glu18-Asn247, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Glu18
Structure / Form
Oligomer
Predicted Molecular Mass
25.8 kDa
SDS-PAGE
28-38 kDa, under reducing conditions

Product Datasheets

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5095-AC

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5095-AC

Formulation Supplied as a 0.2 μm filtered solution in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.
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Background: Adiponectin/Acrp30

Adiponectin, also known as Acrp30, is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity, and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. In this context, adiponectin is an anti-inflammatory agent, but it exerts pro-inflammatory effects in nonmetabolic disorders such as rheumatoid arthritis and inflammatory bowel disease (1-3). Adiponectin interacts with the receptors AdipoR1 and AdipoR2, calreticulin, and Cadherin-13/T-Cadherin, as well as with several growth factors (4-7). Mature mouse adiponectin consists of a 66 amino acid (aa) N-terminal collagenous region and a 137 aa C-terminal C1q-like globular domain which can be cleaved by a leukocyte-derived elastase (8-10). Mature mouse adiponectin shares 83% and 91% amino acid (aa) sequence identity with human and rat adiponectin, respectively. Adiponectin associates into trimers that may assemble into medium molecular weight (MMW) hexamers and then into >300 kDa high molecular weight (HMW) oligomers (11-13). The glycosylation of four hydroxylated lysine residues in the collagenous domain is required for the intracellular formation of HMW complexes (14). The various multimeric forms of adiponectin exhibit distinct tissue specific and gender specific profiles and activities (13, 15).

References
  1. Lara-Castro, C. et al. (2007) Curr. Opin. Lipidol. 18:263.
  2. Tilg, H. and A.R. Moschen (2006) Nat. Rev. Immunol. 6:772.
  3. Fantuzzi, G. (2008) J. Allergy Clin. Immunol. 121:326.
  4. Yamauchi, T. et al. (2007) Nat. Med. 13:332.
  5. Takemura, Y. et al. (2007) J. Clin. Invest. 117:375.
  6. Hug, C. et al. (2004) Proc. Natl. Acad. Sci. 101:10308.
  7. Wang, Y. et al. (2005) J. Biol. Chem. 280:18341.
  8. Scherer, P.E. et al. (1995) J. Biol. Chem. 270:26746.
  9. Hu, E. et al. (1996) J. Biol. Chem. 271:10697.
  10. Waki, H. et al. (2005) Endocrinology 146:790.
  11. Waki, H. et al. (2003) J. Biol. Chem. 278:40352.
  12. Tsao, T.S. et al. (2003) J. Biol. Chem. 278:50810.
  13. Wang, Y. et al. (2008) Biochem. J. 409:623.
  14. Wang, H. et al. (2006) J. Biol. Chem. 281:16391.
  15. Pajvani, U.B. et al. (2003) J. Biol. Chem. 278:9073.
Entrez Gene IDs
9370 (Human); 11450 (Mouse); 246253 (Rat)
Alternate Names
ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; Adiponectin; adiponectin, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; ApM1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28; GBP28apM1; Gelatin-binding protein

Citations for Recombinant Mouse Adiponectin/Acrp30 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. ?-cell-specific overexpression of adiponectin receptor 1 does not improve diabetes mellitus in Akita mice
    Authors: J Choi, H Kobayashi, H Okuda, KH Harada, M Takeda, H Fujimoto, S Yamane, D Tanaka, S Youssefian, N Inagaki, A Koizumi
    PLoS ONE, 2018;13(1):e0190863.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Adiponectin deficiency contributes to the development and progression of benign prostatic hyperplasia in obesity
    Authors: S Fu, H Xu, M Gu, C Liu, Q Wang, X Wan, Y Chen, Q Chen, Y Peng, Z Cai, J Zhou, Z Wang
    Sci Rep, 2017;7(0):43771.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  3. Exercise restores muscle stem cell mobilization, regenerative capacity and muscle metabolic alterations via adiponectin/AdipoR1 activation in SAMP10 mice
    Authors: Aiko Inoue
    J Cachexia Sarcopenia Muscle, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Negative Skeletal Effects of Locally Produced Adiponectin.
    Authors: Abbott M, Roth T, Ho L, Wang L, O'Carroll D, Nissenson R
    PLoS ONE, 2015;10(7):e0134290.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Ultrastructural localization of adiponectin protein in vasculature of normal and atherosclerotic mice.
    Authors: Mori T, Koyama Y, Maeda N, Nakamura Y, Fujishima Y, Matsuda K, Funahashi T, Shimada S, Shimomura I
    Sci Rep, 2014;4(0):4895.
    Species: Mouse
    Sample Types: Antibody
    Applications: Bioassay
  6. Effect of adiponectin on kidney crystal formation in metabolic syndrome model mice via inhibition of inflammation and apoptosis.
    Authors: Fujii, Yasuhiro, Okada, Atsushi, Yasui, Takahiro, Niimi, Kazuhiro, Hamamoto, Shuzo, Hirose, Masahito, Kubota, Yasue, Tozawa, Keiichi, Hayashi, Yutaro, Kohri, Kenjiro
    PLoS ONE, 2013;8(4):e61343.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Bioassay

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