Recombinant Mouse Apolipoprotein H Protein, CF

R&D Systems | Catalog # 6575-AH

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse Apolipoprotein H Protein (6575-AH)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q01339

Structure / Form

Monomer

Applications

Bioactivity
View all Apolipoprotein H/ApoH Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Apolipoprotein H/ApoH protein
Gly20-Cys345, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly20

Predicted Molecular Mass

37.5 kDa

SDS-PAGE

60-65 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.

When Recombinant Mouse Apolipoprotein H/ApoH (Catalog # 6575-AH) binds to Recombinant Mouse LDLR (Catalog # 2255-LD) with an ED50 of < 1.00 μg/mL.

Formulation, Preparation, and Storage

6575-AH
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Apolipoprotein H/ApoH

Apolipoprotein H (ApoH), also known as beta 2‑Glycoprotein I/ beta 2-GPI, is a 50 kDa variably glycosylated member of the complement control superfamily of proteins (1, 2). Mature mouse ApoH consists of four tandem Sushi/SCR repeats followed by one Sushi-like repeat (3, 4). Mature mouse ApoH shares 76% and 42% aa sequence identity with human and rat ApoH, respectively. Hepatocyte-derived ApoH binds directly to negatively charged phospholipids (5). It circulates as a component of lipoprotein particles and as a lipid-free serum protein (6). ApoH also associates with liposomes and apoptotic cell debris, thereby enabling their renal clearance via Megalin uptake (7, 8). Circulating levels of ApoH are postively correlated with triglyceride-rich lipoprotein (VLDL) components in type II diabetes (9). ApoH inhibits thrombosis by blocking the activation of Coagulation Factor XI but also shows procoagulant activity by inhibiting the activation of Protein C (10, 11). ApoH can be cleaved by Plasmin at Lys317 ‑ Thr318, an action that is enhanced by heparin (12, 13). ApoH cleavage reduces its ability to bind phospholipids and inhibit Factor XI activation but confers the ability to bind Plasminogen (10, 12, 14). Cleaved ApoH also demonstrates antiangiogenic activity, whereas intact ApoH does not (14). The production of antibodies against ApoH is a hallmark of Antiphospholipid Syndrome (APS), an autoimmune disorder that leads to hypercoagulability and recurrent miscarriages (15). ApoH binds to the surface antigen of Hepatitis B Virus and is associated with the development of HBV-induced hepatocellular carcinoma (6, 16).

References

  1. Crook, M.A. et al. (2010) Atherosclerosis 209:32.
  2. Miyakis, S. et al. (2004) Thromb. Res. 114:335.
  3. Nonaka, M. et al. (1992) Genomics 13:1082.
  4. Lozier, J. et al. (1984) Proc. Natl. Acad. Sci. 81:3640.
  5. Wurm, H. (1984) Int. J. Biochem. 16:511.
  6. Mehdi, H. et al. (1994) J. Virol. 68:2415.
  7. Chonn, A. et al. (1995) J. Biol. Chem. 270:25845.
  8. Moestrup, S.K. et al. (1998) J. Clin. Invest. 102:902.
  9. Castro, A. et al. (2010) Atherosclerosis 209:201.
  10. Shi, T. et al. (2004) Proc. Natl. Acad. Sci. 101:3939.
  11. Mori, T. et al. (1996) Thromb. Haemost. 75:49.
  12. Hunt, J. et al. (1993) Proc. Natl. Acad. Sci. 90:2141.
  13. Guerin, J. et al. (2002) J. Biol. Chem. 277:2644.
  14. Sakai, T. et al. (2007) Am. J. Pathol. 171:1659.
  15. Adams, M. (2008) Semin. Thromb. Haemost. 34:251.
  16. Jing, X. et al. (2010) J. Cancer Res. Clin. Oncol. 16:1671.

Alternate Names

APOH, B2G1, B2GPI, Beta, BG

Entrez Gene IDs

350 (Human); 11818 (Mouse); 287774 (Rat)

Gene Symbol

APOH

UniProt

Q01339

Additional Apolipoprotein H/ApoH Products

Product Documents for Recombinant Mouse Apolipoprotein H Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse Apolipoprotein H Protein, CF

For research use only

Related Research Areas

Coagulation
Obesity

Citations for Recombinant Mouse Apolipoprotein H Protein, CF

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