Recombinant Mouse Cadherin-9 His-tag Protein, CF

Catalog # Availability Size / Price Qty
1456-CA-050
Recombinant Mouse Cadherin-9 His-tag Protein, CF Binding Activity
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Recombinant Mouse Cadherin-9 His-tag Protein, CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Cadherin-9 is immobilized at 1 μg/mL, 100 μL/well, Recombinant Human Cadherin-6/KCAD Fc Chimera Recombinant Human Cadherin‑6/KCAD Fc Chimera (Catalog # 2715-CA) binds with an ED50 of 0.25-1.5 μg/mL.          
Source
Human embryonic kidney cell, HEK293-derived mouse Cadherin-9 protein
Mouse Cadherin-9
(Gly53-Ala614)
Accession # P70407
HPGGGSGGGSGGGSHHHHHH
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Gly53
Predicted Molecular Mass
64.4 kDa
SDS-PAGE
75-85 kDa, under reducing conditions

Product Datasheets

1456-CA

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Recombinant Mouse Cadherin-9 His-tag Protein, CF Binding Activity View Larger

When Recombinant Mouse Cadherin 9 is immobilized at 1 μg/mL,100 μL/well, Recombinant Human Cadherin-6/KCAD Fc Chimera Recombinant Human Cadherin‑6/KCAD Fc Chimera (Catalog # 2715-CA) binds with an ED50 of0.25-1.5 μg/mL.

SDS-PAGE Recombinant Mouse Cadherin-9 His-tag Protein, CF SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse Cadherin-9 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 75-85 kDa.

Reconstitution Calculator

Reconstitution Calculator

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Background: Cadherin-9

Cadherin-9 (CDH9) is a member of the larger Cadherin superfamily of cell surface glycoproteins originally identified as proteins mediating cell-cell adhesion (1). In humans, there are more than 100 cadherin members divided into distinct families and numerous sub-families (1-3). Cadherins share a general structural architecture with an extracellular domain (ECD) containing 2 or more extracellular Ca2+ binding cadherin repeat (EC) domains, a single-pass transmembrane section, and a short cytoplasmic tail (1-3). Cadherins function by forming homophilic binding interactions through these EC domains to generate both trans and cis dimers (1-3). Mouse CDH9 is categorized as a classical cadherin, containing 5 EC domains, and the ECD shares 94% and 98% amino acid sequence identity with the ECD of human and rat CDH9, respectively. Cadherin-9 is expressed in human brain and kidney, in the limbic system of rats, and in testes and CD4/CD8-double-positive thymocytes of mice (4, 5). Cadherin-9 interacts with alpha and beta catenins, and also interacts with cadherin-6, 9, and 10 in cell aggregation assay (5). High levels of cadherin-9 is observed during renal fibrosis making cadherin-9 a key marker for fibroblasts in healthy and diseased kidney (4). Cadherin-9 plays a critical role in regulating the formation and differentiation of the hippocampal dentate gyrus (DG)-CA3 mossy fiber synapse (3). Loss of CDH9 expression leads to defects in synapse formation and differentiation of specific neural circuits (5). Additionally, disruption to CDH9 and CDH10 genes has been linked autism spectrum disorders (7, 8). Recently, CDH9 has been reported as a potential suppressor of cancer metastasis (9).

References
  1. Gumbiner, B.M. (2005) Nat. Rev. Mol. Cell Biol. 6:622.
  2. Hirano S and Takeichi M. (2012) Physiol Rev. Apr; 92(2):597.
  3. Nollet et al. (2000) J Mol Biol. Jun 9; 299(3):551.
  4. Thedieck, C. et al. (2007) PLoS One. 2(8):e657.
  5. Williams ME et al. (2011) Neuron. Aug. 25; 71(4):640.
  6. Shimoyama, Y. et al. (2000) Biochem. J. 349:159.
  7. Wang K. et al. (2009) Nature 459:528.
  8. Inoue YU and, Inoue T. (2016) Sci Rep. 6:31227.
  9. Yan G. et al. (2017) Scientific Reports volume 7:10023.
Entrez Gene IDs
1007 (Human); 12565 (Mouse); 29163 (Rat)
Alternate Names
cadherin 9, type 2 (T1-cadherin); Cadherin9; Cadherin-9; CDH9; T1-Cadherin

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Primary Antibodies

His Tag Antibody

WB,Simple Western,Flow,AP,CyTOF-ready

Protein Purification Kits

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