Recombinant Mouse CD25/IL-2R alpha Protein, CF

R&D Systems | Catalog # 2438-RM

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse CD25/IL-2R alpha Protein (2438-RM)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q544I2

Applications

Bioactivity
View all CD25/IL-2R alpha Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CD25/IL-2 R alpha protein
Glu22-Lys236, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu22

Predicted Molecular Mass

26 kDa

SDS-PAGE

40-50 kDa, reducing conditions

Activity

Measured by its ability to inhibit the IL-2-dependent proliferation of MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359.
The ED50 for this effect is 0.045-0.3 µg/mL in the presence of 30 ng/mL of recombinant human IL-2.

Reviewed Applications

Read 1 review rated 4 using 2438-RM in the following applications:

Formulation, Preparation, and Storage

2438-RM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD25/IL-2R alpha

IL-2 receptor alpha (IL-2 R alpha ), also known as CD25, is a 55 kDa type I membrane glycoprotein that belongs to the family of cytokine receptors that utilize the common gamma chain subunit ( gamma c). IL-2 R alpha is primarily expressed on activated T cells and on regulatory T cells (Treg) (1-3). The mouse IL-2 R alpha cDNA encodes a 268 amino acid (aa) precursor that includes a 21 aa signal peptide, a 215 aa extracellular domain (ECD) with two Sushi domains, a 21 aa transmembrane segment, and an 11 aa cytoplasmic domain (4, 5). Within the ECD, mouse IL-2 R alpha shares 81% and 58% aa sequence identity with rat and human IL-2 R alpha, respectively. It shares approximately 15% aa sequence identity with IL-4, -7, -9, -15, and -21 receptor subunits that also complex with gamma c. IL-2 R beta (CD122) and gamma c (IL-2 R gamma /CD132) dimerize to form a constitutively expressed intermediate affinity IL-2 receptor (6, 7). By itself, IL-2 R alpha binds IL-2 with low affinity. It associates with IL-2 R beta and gamma c to generate a ternary high affinity IL-2 receptor complex (8). A soluble form of IL-2 R alpha can be generated by proteolytic cleavage of the cell surface receptor, rendering the T cell unresponsive to IL-2 (9, 10). Increased serum levels of soluble IL-2 R alpha are found in some cancers and immune disorders (11). IL-2 R alpha is required for activation induced cell death (AICD) of naive T cells, a mechanism responsible for deleting autoreactive T cell clones (12, 13). IL-2 R alpha is also required for the development of CD4+CD25+ Treg which suppress autoreactive CD4+ T cells, thereby contributing to peripheral T cell homeostasis (12-14).

References

  1. Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
  2. Kovanen, P.E. and W.J. Leonard (2004) Immunol. Rev. 202:67.
  3. Bluestone, J.A. and Q. Tang (2005) Curr. Opin. Immunol. 17:638.
  4. Miller, J. et al. (1985) J. Immunol. 134:4212.
  5. Shimuzu, A. et al. (1985) Nucleic Acids Res. 13:1505.
  6. Hatakeyama, M. et al. (1989) Science 244:551.
  7. Takeshita, T. et al. (1992) Science 257:379.
  8. Wang, X. et al. (2005) Science 310:1159.
  9. Wagner, D.K. et al. (1986) J. Immunol. 137:592.
  10. Schulz, O. et al. (1998) J. Exp. Med. 187:271.
  11. Witkowska, A.M. (2005) Mediat. Inflamm. 2005:121.
  12. Willerford, D.M. et al. (1995) Immunity 3:521.
  13. Van Parijs, L. et al. (1997) J. Immunol. 158:3738.
  14. Almeida, A.R.M. et al. (2002) J. Immunol. 169:4850.

Long Name

Interleukin 2 Receptor alpha

Alternate Names

CD25, IL-2 R alpha, IL-2Ra, IL2R alpha, IL2RA

Entrez Gene IDs

3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine); 102123605 (Cynomolgus Monkey)

Gene Symbol

IL2RA

UniProt

Q544I2 (Mouse)

Additional CD25/IL-2R alpha Products

Product Documents for Recombinant Mouse CD25/IL-2R alpha Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse CD25/IL-2R alpha Protein, CF

For research use only

Citations for Recombinant Mouse CD25/IL-2R alpha Protein, CF

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  • Recombinant Mouse CD25/IL-2R alpha Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 03/18/2020
    The recombinant mouse CD25/IL-2 receptor alpha is able to bind mouse IL2 in an octet binding assay
    Recombinant Mouse CD25/IL-2R alpha Protein, CF 2438-RM

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