Recombinant Mouse Contactin-4 Protein, CF

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Recombinant Mouse Contactin-4 Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Immobilized Recombinant Mouse Contactin-4 (as a 3 μL droplet containing 100 ng) on a nitrocellulose coated microplate is sufficient to significantly enhance neurite outgrowth.
Chinese Hamster Ovary cell line, CHO-derived mouse Contactin-4 protein
Asp19-Gly999 (Ile64Leu), with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
109.5 kDa
135-155 kDa, reducing conditions

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 300 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Contactin-4

Contactin-4 (CNTN4), also known as BIG-2 (brain-derived immunoglobulin superfamily molecule 2), is an axonal cell adhesion molecule (AxCAM) that belongs to the contactin family, a subfamily of the Ig superfamily (1, 2). The contactin family comprises six members (CNTN1/F3, CNTN2/TAG-1, CNTN3/BIG-1, CNTN4/BIG-2, CNTN5/NB-2 and CNTN6/NB-3) that are characterized by the presence of six Ig like domains, four fibronectin type III-like repeats, and a glycosylphosphatidylinositol (GPI)-anchoring domain (1-4). Contactins are membrane-anchored proteins that can be released as soluble proteins by GPI-specific phospholipase D and are able to promote neurite outgrowth in their soluble form (2). Potential 1026, 705, and 498 amino acid (aa) isoforms of mouse CNTN4 have been described (1, 5). Only the longest isoform includes the C-terminal GPI anchoring sequence. It shares 97% aa identity with rat and 95% aa identity with human, equine and bovine CNTN4 in its mature 981 aa form. It also shares 42-64% aa identity with other CNTN family members, showing highest identity with CNTN3. CNTN4 is expressed throughout the brain, but minor amounts are also detected in small intestine, thyroid, uterus and testis (2, 4). Family members display overlapping but distinct expression patterns in rat brain and are suggested to influence the formation and maintenance of specific neuronal networks (2). In the olfactory bulb, CNTN4 is expressed in specific sensory neurons in a mosaic pattern that is closely correlated with odorant receptor choice, and is thought important for odor mapping (6, 7). In humans, disruption of CNTN4 has been implicated in the 3p deletion syndrome characterized by developmental and growth delay, and in autism spectrum disorder (8-10).

  1. Mimmack, M. L. et al. (1997) Brain Res. Mol. Brain Res. 47:345.
  2. Yoshihara, Y. et al. (1995) J. Neurobiol. 28:51.
  3. Ogawa, J. et al. (1996) Neurosci. Lett. 218:173.
  4. Hansford, L.M. et al. (2003) Cytogenet. Genome Res. 101:17.
  5. Swissprot Accession #Q69Z26.
  6. Saito, H. et al. (1998) Brain Res. Dev. Brain Res. 110:69.
  7. Kaneko-Goto, T. et al. (2008) Neuron 57:834.
  8. Fernandez, T. et al. (2004) Am. J. Hum. Genet. 74:1286.
  9. Fernandez, T. et al. (2008) Am. J. Hum. Genet. 146A:2746.
  10. Roohi, J. et al. (2008) J. Med Genet. doi:10.1136/jmg.2008.057505.
Entrez Gene IDs
152330 (Human); 269784 (Mouse)
Alternate Names
AXCAM; axonal-associated cell adhesion molecule; BIG-2; BIG-2Brain-derived immunoglobulin superfamily protein 2; CNTN4; contactin 4; Contactin4; Contactin-4; MGC33615; neural cell adhesion protein BIG-2


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