Recombinant Mouse CRISP-1 Protein, CF Summary
Gln20-His244, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 200 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Cysteine‑rich secretory protein 1 (CRISP‑1), also known as Acidic Epididymal Glycoprotein 1 (AEG1) and Sperm‑Coating Glycoprotein 1 (SCP1), is a 32 kDa protein that is involved in male reproductive biology (1, 2). CRISPs belong to the CAP superfamily of molecules that also includes several snake, insect, and lizard venom proteins (3). Structurally, CRISPs consist of an N‑terminal SCP/CAP domain, a hinge region, and a Cys‑rich domain with 16 invariant cysteine residues (4). CRISPs are expressed in discrete but overlapping regions of the male reproductive tract and play a role in spermatozoa adhesion with Sertoli cells and oocytes, decapacitation, and the acrosomal reaction (1). Expression patterns, genomic structure, and sequence conservation indicate that the likely ortholog of human CRISP‑1 in mouse and rat is CRISP‑4 (5, 6). Mature mouse CRISP‑1 shares 68% amino acid (aa) sequence identity with rat CRISP‑1. It shares 55%, 73%, and 43% aa sequence identity with mouse CRISP‑2, ‑3, and ‑4, respectively. Rodent CRISP‑1 is secreted by the epididymal epithelium and associates with the surface of spermatozoa in the epididymis where it inhibits capacitation (7 ‑ 9). It is released from the sperm cell surface once capacitation begins in the female reproductive tract or in vitro (10). CRISP‑1 is involved in the interaction of spermatozoa with the oocyte zona pellucida as well as in the fusion of sperm and egg (11, 12). CRISP‑1 is additionally expressed in the lower medulla of hair shafts and under androgen control in the submandibular salivary glands of male mice (13 ‑ 15).
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- Peterson, R.L. et al. (2005) J. Investig. Dermatol. Symp. Proc. 10:238.
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