Recombinant Mouse CRISP-1 Protein, CF

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R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse CRISP-1 Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Measured by its ability to inhibit proliferation/survival of the 3A‑sub E human placenta cell line. The ED50 for this effect is 0.12-0.6 μg/mL.
Mouse myeloma cell line, NS0-derived mouse CRISP-1 protein
Gln20-His244, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Gln20 predicted: No results obtained, sequencing might be blocked.
Predicted Molecular Mass
26.4 kDa
30 kDa, reducing conditions.

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: CRISP-1

Cysteine‑rich secretory protein 1 (CRISP‑1), also known as Acidic Epididymal Glycoprotein 1 (AEG1) and Sperm‑Coating Glycoprotein 1 (SCP1), is a 32 kDa protein that is involved in male reproductive biology (1, 2). CRISPs belong to the CAP superfamily of molecules that also includes several snake, insect, and lizard venom proteins (3). Structurally, CRISPs consist of an N‑terminal SCP/CAP domain, a hinge region, and a Cys‑rich domain with 16 invariant cysteine residues (4). CRISPs are expressed in discrete but overlapping regions of the male reproductive tract and play a role in spermatozoa adhesion with Sertoli cells and oocytes, decapacitation, and the acrosomal reaction (1). Expression patterns, genomic structure, and sequence conservation indicate that the likely ortholog of human CRISP‑1 in mouse and rat is CRISP‑4 (5, 6). Mature mouse CRISP‑1 shares 68% amino acid (aa) sequence identity with rat CRISP‑1. It shares 55%, 73%, and 43% aa sequence identity with mouse CRISP‑2, ‑3, and ‑4, respectively. Rodent CRISP‑1 is secreted by the epididymal epithelium and associates with the surface of spermatozoa in the epididymis where it inhibits capacitation (7 ‑ 9). It is released from the sperm cell surface once capacitation begins in the female reproductive tract or in vitro (10). CRISP‑1 is involved in the interaction of spermatozoa with the oocyte zona pellucida as well as in the fusion of sperm and egg (11, 12). CRISP‑1 is additionally expressed in the lower medulla of hair shafts and under androgen control in the submandibular salivary glands of male mice (13 ‑ 15).

  1. Koppers, A.J. et al. (2011) Asian J. Androl. 13:111.
  2. Gibbs, G.M. et al. (2008) Endocr. Rev. 29:865.
  3. Yamazaki, Y. and T. Morita (2004) Toxicon 44:227.
  4. Guo, M. et al. (2005) J. Biol. Chem. 280:12405.
  5. Jalkanen, J. et al. (2005) Biol. Reprod. 72:1268.
  6. Nolan, M.A. et al. (2006) Biol. Reprod. 74:984.
  7. Charest, N.J. et al. (1989) Endocrinology 125:942.
  8. Maldera, J.A. et al. (2011) Biol. Reprod. 85:503.
  9. Nixon, B. et al. (2006) Biol. Reprod. 74:275.
  10. Roberts, K.P. et al. (2003) Biol. Reprod. 69:572.
  11. Busso, D. et al. (2007) Biol. Reprod. 77:848.
  12. Da Ros, V.G. et al. (2008) Dev. Biol. 320:12.
  13. Haendler, B. et al. (1993) Endocrinology 133:192.
  14. Mizuki, N. and M. Kasahara (1992) Mol. Cell. Endocrinol. 89:25.
  15. Peterson, R.L. et al. (2005) J. Investig. Dermatol. Symp. Proc. 10:238.
Long Name
Cysteine-rich Secretory Protein 1
Entrez Gene IDs
167 (Human); 11571 (Mouse); 64827 (Rat)
Alternate Names
Acidic epididymal glycoprotein homolog; acidic epididymal glycoprotein-like 1; AEGL1; AEG-related protein; ARPAEG-like protein; CRISP1; CRISP-1; CRISP-1AEGL1HSCRISP1D; cysteine-rich secretory protein 1; cysteine-rich secretory protein-1 delta; HSCRISP1G; HUMARP


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