Recombinant Mouse CRISP-2 Protein, CF

Catalog # Availability Size / Price Qty
4576-CR-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse CRISP-2 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit proliferation/survival of the 3A‑sub E human placenta cell line. The ED50 for this effect is 0.5-2.5 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse CRISP-2 protein
Lys23-His243 with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Lys23
Predicted Molecular Mass
25.9 kDa
SDS-PAGE
27 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4576-CR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

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Background: CRISP-2

CRISP-2, also known as Tpx-1, is one of several CRISPs (cysteine-rich secretory proteins) that contain 16 invariant cysteine residues and show structural homology to snake, insect, and lizard venom proteins. These proteins consist of an N-terminal SCP/CAP domain, a hinge region, and a cysteine-rich CRISP domain. CRISPs are expressed in discrete but overlapping regions of the male reproductive tract and play a role in spermatozoa adhesion with Sertoli cells and oocytes, decapacitation, and the acrosome reaction (1, 2). Mature mouse CRISP‑2 shares 73% and 86% aa sequence identity with human and rat CRISP-2, respectively (3). CRISP-2 is expressed in late pachytene spermatocytes, round and elongated spermatids, and mature spermatozoa (4, 5). It is found in cytoplasmic granulated bodies and in association with Gametogenetin 1, SHTAP, outer dense fibers of the tail, and the acrosome (5-9). CRISP-2 is involved in spermatocyte interactions with Sertoli cells as well as in sperm-egg fusion (7, 8, 10). It regulates calcium flux during sperm capacitation by increasing or decreasing the open time of the ryanodine receptors RyR1 and RyR2, respectively (11).

References
  1. Koppers, A.J. et al. (2011) Asian J. Androl. 13:111.
  2. Gibbs, G.M. et al. (2008) Endocr. Rev. 29:865.
  3. Kasahara, M. et al. (1989) Genomics 5:527.
  4. O’Bryan, M.K. et al. (1998) Mol. Reprod. Dev. 50:313.
  5. O’Bryan, M.K. et al. (2001) Mol. Reprod. Dev. 58:116.
  6. Jamsai, D. et al. (2008) Reproduction 135:751.
  7. Busso, D. et al. (2007) Biol. Reprod. 76:701.
  8. Busso, D. et al. (2005) Mol. Hum. Reprod. 11:299.
  9. Jamsai, D. et al. (2010) Biol. Cell 102:93.
  10. Maeda, T. et al. (1998) Biochem. Biophys. Res. Commun. 248:140.
  11. Gibbs, G.M. et al. (2006) J. Biol. Chem. 281:4156.
Long Name
Cysteine-rich Secretory Protein 2
Entrez Gene IDs
7180 (Human); 22024 (Mouse)
Alternate Names
Cancer/testis antigen 36; CRISP2; CRISP-2; CRISP-2GAPDL5; CT36TSP1; cysteine-rich secretory protein 2; GAPDL5; glyceraldehyde-3-phosphate dehydrogenase-like 5; testis specific protein 1 (probe H4-1 p3-1); Testis-specific protein TPX-1; TPX1; TPX1MGC111136; TSP1

Citation for Recombinant Mouse CRISP-2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Zinc binding regulates amyloid-like aggregation of GAPR-1
    Authors: J Sheng, NK Olrichs, WJ Geerts, X Li, AU Rehman, BM Gadella, DV Kaloyanova, B Helms
    Biosci. Rep., 2019;0(0):.

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