Recombinant Mouse CRISP-4 Protein, CF

Catalog # Availability Size / Price Qty
5017-CR-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse CRISP-4 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of NTera‑2 human testicular embryonic carcinoma cells.

The ED50 for this effect is 0.3-1.5 μg/mL after a 1 hour incubation at
37 °C.

Optimal dilutions should be determined by each laboratory for each application.

Source
Chinese Hamster Ovary cell line, CHO-derived mouse CRISP-4 protein
Pro22-Lys250, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Leu23
Predicted Molecular Mass
27.2 kDa
SDS-PAGE
34-37 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5017-CR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CRISP-4

CRISP-4 is one of several CRISPs (cysteine-rich secretory proteins) that contain 16 invariant cysteine residues and show structural homolgy to snake, insect, and lizard venom proteins. These proteins consist of an N-terminal SCP/CAP domain, a hinge region, and a cysteine-rich CRISP domain. CRISPs are expressed in discrete but overlapping regions of the male reproductive tract and play a role in spermatozoa adhesion with Sertoli cells and oocytes, decapacitation, and the acrosomal reaction (1 - 3). CRISP-4 facilitates the progesterone-induced acrosomal reaction by inhibiting calcium conductance through the TRPM8 channel on sperm (4). Mouse CRISP-4 is secreted as a 32 kDa protein in the epididymis (primarily the caput region), vas deferens, and seminal vesicle (5, 6). It is also associated with spermatozoa from the testis and caudal epididymis as a 26 - 28 kDa molecule (6). Its expression is induced at the onset of puberty in an androgen-dependent fashion (5). Mouse and rat CRISP-1 are additionally expressed in salivary glands, while CRISP-4 is not (6). Expression patterns, genomic structure, and sequence conservation indicate that CRISP-4 in mouse and rat are the likely orthologs of human CRISP-1 (5, 6). Mature mouse CRISP-4 shares 90% and 64% aa sequence identity with rat CRISP-4 and human CRISP-1, respectively (5). Mature mouse CRISP-4 shares 38% - 43% aa sequence identity with mouse CRISP-1, -2, and -3.

References
  1. Koppers, A.J. et al. (2011) Asian J. Androl. 13:111.
  2. Gibbs, G.M. et al. (2008) Endocr. Rev. 29:865.
  3. Maeda, T. et al. (1998) Biochem. Biophys. Res. Commun. 248:140.
  4. Gibbs, G.M. et al. (2011) Proc. Natl. Acad. Sci. 108:7034.
  5. Jalkanen, J. et al. (2005) Biol. Reprod. 72:1268.
  6. Nolan, M.A. et al. (2006) Biol. Reprod. 74:984.
Long Name
Cysteine-rich Secretory Protein 4
Entrez Gene IDs
78081 (Mouse); 654517 (Rat)
Alternate Names
CRISP4; CRISP-4

Citation for Recombinant Mouse CRISP-4 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Zinc binding regulates amyloid-like aggregation of GAPR-1
    Authors: J Sheng, NK Olrichs, WJ Geerts, X Li, AU Rehman, BM Gadella, DV Kaloyanova, B Helms
    Biosci. Rep., 2019;0(0):.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: ThT Fluorescence

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