Recombinant Mouse DNER Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
2254-DN-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (3)
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Recombinant Mouse DNER Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit Pleiotrophin-induced neurite outgrowth of E16-E18 rat embryonic cortical neurons. When 4 x 104 neurons/well are added to 96-well plate containing serial dilutions of Recombinant Mouse DNER Fc Chimera and immobilized Recombinant Mouse Pleiotrophin/PTN (1 μg/mL) (Catalog # 6580-PL), neurite outgrowth is significantly inhibited in a dose dependent manner. The ED50 for this effect is 0.5 ‑ 2.5 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse DNER protein
Mouse DNER
(Ala26 - His637)
Accession # NP_690879
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Ala26
Predicted Molecular Mass
91.4 kDa (reduced)
SDS-PAGE
140-160 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2254-DN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: DNER

DNER (Delta/Notch-like EGF-related receptor), also known as BET (brain-specific EGF-like transmembrane protein), is a type I transmembrane glycoprotein of the Notch/Delta family (1, 2). Mouse DNER has been detected as 90, 120 and 150 kDa forms which are probably variably glycosylated (1, 2). DNER is specifically expressed on nonaxonal areas of post-mitotic neurons, especially Purkinje cells, but also cortical and hippocampal pyramidal neurons and immature cerebellar granule cells (1 ‑ 5). After expression on the cell surface, DNER is removed from axonal membranes, creating its somatodendritic specificity (1, 3, 6). A portion of DNER is found within endosomes (1, 3, 6). Mouse DNER cDNA encodes 737 amino acids (aa) that include a 25 aa signal sequence, a 615 aa extracellular domain (ECD) containing ten distinct Delta/Notch-like EGF-like repeats, a 21 aa transmembrane sequence, and a 76 aa cytoplasmic domain. The mouse DNER ECD shares 89%, 96% and 86% aa sequence identity with human, rat and bovine DNER, respectively. DNER is a Notch ligand, but is considered a non-classical ligand because it lacks the usual DSL Notch binding motif (5, 7). Instead, Notch interacts with the two EGF-like repeats closest to the N-terminus of DNER (5). DNER also associates with protein tyrosine phosphatase zeta (PTP zeta ), which is the receptor of pleiotrophin (PTN). PTP zeta -PTN-DNER signaling has been implicated in the regulation of neuritogenesis (3). Mice lacking DNER show impaired cerebellar functions and delayed Purkinje cell-mediated maturation of Notch-expressing Bergmann glia during cerebellar development (4, 5). Expression of DNER in glioblastoma stem-like cells inhibits formation of neurospheres in vitro, induces differentiation and inhibits growth of xenografts, thus acting as a tumor suppressor (7). Expression of DNER in adipose‑derived human mesenchymal stem cells and mouse auditory hair cells have also been shown (8, 9).

References
  1. Eiraku, M. et al. (2002) J. Biol. Chem. 277:25400.
  2. Nishizumi, H. et al. (2002) NeuroReport 13:909.
  3. Fukazawa, N. et al. (2008) Mol. Cell. Biol. 28:4494.
  4. Tohgo, A. et al. (2005) Mol. Cell. Neurosci. 31:326.
  5. Eiraku, M. et al. (2005) Nat. Neurosci. 8:873.
  6. Kurisu, J. et al. (2010) J. Neurochem. 113:1598.
  7. Sun, P. et al. (2009) Stem Cells 27:1473.
  8. Park, J-R. et al. (2010) Cell Prolif. 43:19.
  9. Hartman, B.H. et al. (2010) J. Assoc. Res. Otolaryngol. 11:187.
Long Name
Delta/Notch-like EGF Repeat-containing Transmembrane
Entrez Gene IDs
92737 (Human); 227325 (Mouse)
Alternate Names
bet; delta and Notch-like epidermal growth factor-related receptor; delta/notch-like EGF repeat containing; delta-notch-like EGF repeat-containing transmembrane; DNER; H_NH0150O02.1; UNQ26; WUGSC:H_NH0150O02.1

Citations for Recombinant Mouse DNER Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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  1. Post-arrest stalling rather than crawling favors CD8(+) over CD4(+) T-cell migration across the blood-brain barrier under flow in vitro
    Authors: Henriette Rudolph
    Eur J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Delta/Notch-Like EGF-Related Receptor (DNER) Is Not a Notch Ligand
    PLoS ONE, 2016;11(9):e0161157.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Migration of encephalitogenic CD8 T cells into the central nervous system is dependent on the alpha4beta1-integrin.
    Authors: Martin-Blondel G, Pignolet B, Tietz S, Yshii L, Gebauer C, Perinat T, Van Weddingen I, Blatti C, Engelhardt B, Liblau R
    Eur J Immunol, 2015;45(12):3302-12.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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