Recombinant Mouse Endorepellin His-tag Protein, CF

Catalog # Availability Size / Price Qty
9987-ER-050
Recombinant Mouse Endorepellin His-tag Protein, CF Bioactivity
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Recombinant Mouse Endorepellin His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. The ED50 for this effect is 0.75-4.5 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Endorepellin/Perlecan protein
Glu3683-Ser4383, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Glu3683
Predicted Molecular Mass
75.8 kDa
SDS-PAGE
85-95 kDa, reducing conditions

Product Datasheets

9987-ER

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 300 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Recombinant Mouse Endorepellin His-tag Protein, CF Bioactivity View Larger

Immobilized Recombinant Mouse Endorepellin supports theadhesion of SVEC4‑10 mouse vascular endothelial cells. The ED50 for thiseffect is 0.75‑4.5 μg/mL.

SDS-PAGE Recombinant Mouse Endorepellin His-tag Protein, CF SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse Endorepellin/Perlecan was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 85-95 kDa.

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Background: Endorepellin/Perlecan

Endorepellin is an 80 kDa glycoprotein derived from the C-terminal end of the heparan sulfate proteoglycan Perlecan. Human Perlecan is an approximately 850 kDa basement membrane heparan sulfate proteoglycan containing multiple LDLR, EGF-like, Laminin-like, and immunoglobulin-like domains. Mouse Perlecan lacks several of the EGF, Laminin-like and Ig-like domains found in the human protein (1-3). Mouse Endorepellin, also termed Domain V (DV) of Perlecan, consists of three Laminin G domains separated by a single EGF-like domain. Mouse Endorepellin shares 89% amino acid sequence identity with human Endorepellin. Endorepellin has been shown to broadly inhibit angiogenesis, including endothelial cell migration, collagen-induced capillary morphogenesis, and blood vessel growth (4). Endostatin, an inhibitor of Endorepellin, has been shown to bind Endorepellin directly and block its anti-angiogenic activity (4). Endorepellin can be further processed into a 26 kDa fragment, termed LG3, containing the third Laminin G-like domain of Endorepellin. LG3 possesses anti-angiogenic activity of its own and its release is mediated by either Cathepsin L or BMP-1/Tolloid family cleavage (4-7). Endorepellin binds to Integrin alpha 2/ beta 1, preventing the integrin-dependent adhesion of vascular endothelial cells (EC) to fibronectin and collagen I (7, 8). Additionally, Endorepellin binds to VEGF R1 and VEGF R2 on EC (10). Its binding to VEGF R2 and Integrin alpha 2/beta 1 on EC induces the association and down‑regulation of both proteins, resulting in a reduction in EC migration and overall antiangiogenic activity (4, 7-10). Endorepellin has been shown to disrupt tumor vasculature and inhibit tumor growth, invasion and angiogenesis (8, 11), and it has recently been proposed as a potential stroke therapy by helping with brain repair following injury (12).

References
  1. Whitelock, J.M. et al. (2008) Biochemistry 47:11174.
  2. Murdoch, A.D. et al. (1992) J. Biol. Chem. 267:8544.
  3. Noonan, D.M. et al. (1991) J. Biol. Chem. 266:22939.
  4. Mongiat, M. et al. (2003) J. Biol. Chem. 278:4238.
  5. Cailhier, J.-F. et al. (2008) J. Biol. Chem. 283:27220.
  6. Gonzalez, E.M. et al. (2005) J. Biol. Chem. 280:7080.
  7. Bix, G. et al. (2004) J. Cell Biol. 166:97.
  8. Woodall, B.P. et al. (2008) J. Biol. Chem. 283:2335.
  9. Goyal, A. et al. (2011) J. Biol. Chem. 286:25947.
  10. Nystrom, A. et al. (2009) Blood 114:4897.
  11. Bix, G. et al. (2006) J. Natl. Cancer Inst. 98:1634.
  12. Bix G. (2013) ACS Chem Neurosci. 4:370.
Entrez Gene IDs
3339 (Human); 15530 (Mouse)
Alternate Names
basement membrane-specific heparan sulfate proteoglycan core protein; endorepellin (domain V region); Endorepellin; heparan sulfate proteoglycan 2; HSPG; HSPG2; perlecan proteoglycan; Perlecan; PLCSchwartz-Jampel syndrome 1 (chondrodystrophic myotonia); PRCAN; SJA; SJS; SJS1

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Primary Antibodies

His Tag Antibody

WB,Simple Western,Flow,AP,CyTOF-ready

Protein Purification Kits

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