Recombinant Mouse Frizzled-10 Fc Chimera Protein, CF

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Recombinant Mouse Frizzled-10 Fc Chimera Protein, CF Bioactivity
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Recombinant Mouse Frizzled-10 Fc Chimera Protein, CF Summary

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to bind biotinylated recombinant mouse Wnt-3a in a functional ELISA with an ED50 of 10-60 ng/mL.
Mouse myeloma cell line, NS0-derived mouse Frizzled-10 protein
Mouse Frizzled-10
Accession # Q8BKG4
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
44 kDa
53-61 kDa, reducing conditions

Product Datasheets

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Bioactivity Recombinant Mouse Frizzled-10 Fc Chimera Protein, CF Bioactivity View Larger

Recombinant Mouse Frizzled-10 Fc Chimera (Catalog # 9247-FZ) binds to Biotinylated Recombinant Mouse
Wnt-3a in an ELISA Binding Assay. When Biotinylated Recombinant Mouse Wnt-3a is coated on a streptavidin coated ELISA plate, Recombinant Mouse Frizzled-10 Fc Chimera binds with an ED50 = 10-60 ng/mL.

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Background: Frizzled-10

Frizzled-10, also known as CD350, is a 68 kDa seven pass transmembrane glycoprotein in the frizzled family of Wnt receptors (1, 2). The N-terminal extracellular region of Frizzled-10 contains a cysteine-rich domain that comprises the Wnt binding domain and mediates receptor oligomerization (3-5). The C-terminal cytoplasmic tail contains a PDZ-interaction motif (3). Within the cysteine-rich domain, human Frizzled-10 shares 94% and 99% aa sequence identity with human and rat Frizzled-10, respectively. Frizzled-10 is expressed during embryogenesis in the primitive streak, dorsal neural tube, developing brain, limb bud, and airway epithelium (6-12). It is induced by Shh and colocalizes with Shh and Wnt-7a in the neural tube (13, 14). In the adult, Frizzled-10 is expressed in placenta, gastric glands, and colon and renal tubule epithelial cells (4). Frizzled-10 associates with LRP5 and transduces Wnt-1, -7a, -7b, and -8 signals, resulting in the stabilization of cytoplasmic beta-Catenin (11, 12, 14). It is important for the differentiation of sensory neurons (12). Frizzled-10 is also up-regulated in some cancers and transformed cell lines (4, 15). It binds hypoxia inducible gene 2, which promotes oncogenic Wnt signaling and functions as an autocrine growth factor for renal cell carcinomas (16).

  1. Kikuchi, A. et al. (2007) Cell. Signal. 19:659.
  2. Wang, H. et al. (2006) Cell. Signal. 18:934.
  3. Koike, J. et al. (1999) Biochem. Biophys. Res. Commun. 262:39.
  4. Nagayama, S. et al. (2005) Oncogene 24:6201.
  5. Dann, C.E. et al. (2001) Nature 412:86.
  6. Kemp, C.R. et al. (2007) Dev. Dyn. 236:2011.
  7. Kawakami, Y. et al. (2000) Mech. Dev. 91:375.
  8. Nasevicius, A. et al. (2000) Mech. Dev. 92:311.
  9. Moriwaki, J. et al. (2000) Biochem. Biophys. Res. Commun. 278:377.
  10. Stark, M.R. et al. (2000) Mech. Dev. 93:195.
  11. Wang, Z. et al. (2005) Mol. Cell. Biol. 25:5022.
  12. Garcia-Morales, C. et al. (2009) Dev. Biol. 335:143.
  13. Nunnally, A.P. and B.A. Parr (2004) Dev. Genes Evol. 214:144.
  14. Kawakami, Y. et al. (2000) Dev. Growth Differ. 42:561.
  15. Saitoh, T. et al. (2002) Int. J. Oncol. 20:117.
  16. Togashi, A. et al. (2005) Cancer Res. 65:4817.
Entrez Gene IDs
11211 (Human); 93897 (Mouse); 64512 (Rat)
Alternate Names
CD350 antigen; CD350; frizzled (Drosophila) homolog 10; frizzled homolog 10 (Drosophila); Frizzled10; Frizzled-10; Fz10; FZ-10; FZD10; FzE7; hFz10


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