Recombinant Mouse Frizzled-10 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
9247-FZ-050
Recombinant Mouse Frizzled-10 Fc Chimera Protein, CF Bioactivity
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Recombinant Mouse Frizzled-10 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind biotinylated recombinant mouse Wnt-3a in a functional ELISA with an ED50 of 10-60 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Frizzled-10 protein
Mouse Frizzled-10
(Ile22-Phe166)
Accession # Q8BKG4
IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Ile22
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
44 kDa
SDS-PAGE
53-61 kDa, reducing conditions

Product Datasheets

9247-FZ

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Mouse Frizzled-10 Fc Chimera Protein, CF Bioactivity View Larger

Recombinant Mouse Frizzled-10 Fc Chimera (Catalog # 9247-FZ) binds to Biotinylated Recombinant Mouse
Wnt-3a in an ELISA Binding Assay. When Biotinylated Recombinant Mouse Wnt-3a is coated on a streptavidin coated ELISA plate, Recombinant Mouse Frizzled-10 Fc Chimera binds with an ED50 = 10-60 ng/mL.

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Background: Frizzled-10

Frizzled-10, also known as CD350, is a 68 kDa seven pass transmembrane glycoprotein in the frizzled family of Wnt receptors (1, 2). The N-terminal extracellular region of Frizzled-10 contains a cysteine-rich domain that comprises the Wnt binding domain and mediates receptor oligomerization (3-5). The C-terminal cytoplasmic tail contains a PDZ-interaction motif (3). Within the cysteine-rich domain, human Frizzled-10 shares 94% and 99% aa sequence identity with human and rat Frizzled-10, respectively. Frizzled-10 is expressed during embryogenesis in the primitive streak, dorsal neural tube, developing brain, limb bud, and airway epithelium (6-12). It is induced by Shh and colocalizes with Shh and Wnt-7a in the neural tube (13, 14). In the adult, Frizzled-10 is expressed in placenta, gastric glands, and colon and renal tubule epithelial cells (4). Frizzled-10 associates with LRP5 and transduces Wnt-1, -7a, -7b, and -8 signals, resulting in the stabilization of cytoplasmic beta-Catenin (11, 12, 14). It is important for the differentiation of sensory neurons (12). Frizzled-10 is also up-regulated in some cancers and transformed cell lines (4, 15). It binds hypoxia inducible gene 2, which promotes oncogenic Wnt signaling and functions as an autocrine growth factor for renal cell carcinomas (16).

References
  1. Kikuchi, A. et al. (2007) Cell. Signal. 19:659.
  2. Wang, H. et al. (2006) Cell. Signal. 18:934.
  3. Koike, J. et al. (1999) Biochem. Biophys. Res. Commun. 262:39.
  4. Nagayama, S. et al. (2005) Oncogene 24:6201.
  5. Dann, C.E. et al. (2001) Nature 412:86.
  6. Kemp, C.R. et al. (2007) Dev. Dyn. 236:2011.
  7. Kawakami, Y. et al. (2000) Mech. Dev. 91:375.
  8. Nasevicius, A. et al. (2000) Mech. Dev. 92:311.
  9. Moriwaki, J. et al. (2000) Biochem. Biophys. Res. Commun. 278:377.
  10. Stark, M.R. et al. (2000) Mech. Dev. 93:195.
  11. Wang, Z. et al. (2005) Mol. Cell. Biol. 25:5022.
  12. Garcia-Morales, C. et al. (2009) Dev. Biol. 335:143.
  13. Nunnally, A.P. and B.A. Parr (2004) Dev. Genes Evol. 214:144.
  14. Kawakami, Y. et al. (2000) Dev. Growth Differ. 42:561.
  15. Saitoh, T. et al. (2002) Int. J. Oncol. 20:117.
  16. Togashi, A. et al. (2005) Cancer Res. 65:4817.
Entrez Gene IDs
11211 (Human); 93897 (Mouse); 64512 (Rat)
Alternate Names
CD350 antigen; CD350; frizzled (Drosophila) homolog 10; frizzled homolog 10 (Drosophila); Frizzled10; Frizzled-10; Fz10; Fz-10; FZD10; FzE7; hFz10

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