Recombinant Mouse Gas6 (Full Length) Protein, CF

Catalog # Availability Size / Price Qty
8310-GS-050
Recombinant Mouse Gas6 (Full Length) Protein, CF Bioactivity
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Product Details
Citations (1)
FAQs
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Reviews (1)

Recombinant Mouse Gas6 (Full Length) Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to down regulate the expression of Axl in DU145 human prostate carcinoma cells. Mishra, A. et al. (2012) Mol. Cancer Res. 10:703. The ED50 for this effect is 20-100 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Gas6 protein
Ala46-Pro673, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ala46 & Tyr47
Predicted Molecular Mass
71 kDa
SDS-PAGE
78-94 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8310-GS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity View Larger

Recombinant Mouse Gas6 (Catalog # 8310-GS) down regulates the expression of Axl in DU145 human prostate carcinoma cells. The ED50for this effect is 20-100 ng/mL.

SDS-PAGE View Larger

1 μg/lane of Recombinant Mouse Gas6 (Full Length, Catalog # 8310-GS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing R bands at 85.4 and 69.2 kDa and NR band at 64.4 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Gas6

Gas6 (Growth Arrest Specific 6) is a secreted 75 kDa multimodular protein that is up-regulated by a wide variety of cell types in response to growth arrest. It is expressed by endothelial cells, fibroblasts, neurons, smooth muscle cells, and platelets, and it plays a role in vascular, thrombotic, atherosclerotic, inflammatory, autoimmune, renal, and cancer pathologies (1, 2). Both Gas6 and the related Protein S contain an extensively gamma -carboxylated N-terminal Gla domain, four EGF-like repeats, and two C-terminal Laminin G-like domains. And like Protein S, Gas6 is dependent upon vitamin K for activity. Within the Gla, EGF-like, and Laminin G-like domains, mouse Gas6 shares 83%, 94%, and 41% aa sequence identity with the equivalent regions in human Gas6, rat Gas6, and mouse Protein S, respectively. Gas6 binds to and induces signaling through the receptor tyrosine kinase TAM subfamily, which includes Axl, Dtk/Tyro3, and Mer (3). Shed soluble forms of Axl and Mer retain the ability to bind Gas6 and function as decoy receptors (4, 5). Gas6 participates in tissue homeostasis by protecting cells from stress-induced apoptosis and promoting apoptotic cell phagocytosis. The affinity of the gamma -carboxylated Gla domain for phosphatidylserine contributes to the role of Gas6 in phagocytosis as well as the cellular entry of select viruses (6, 7). Gas6 can function as a pro-inflammatory molecule by promoting platelet activation (8, 9) and can also inhibit inflammatory cytokine production from monocytes, macrophages, and microglia (10). In addition, Gas6 induces the proliferation of cardiac fibroblasts, Schwann cells, vascular smooth muscle cells, and the differentiation of NK cell precursors (11-14). It also inhibits VEGF-induced angiogenesis (15) and can have either positive or negative effects on tumor cell proliferation and invasion (16, 17).

References
  1. Laurance, S. et al. (2012) Adv. Nutr. 3:196.
  2. Manfioletti, G. et al. (1993) Mol. Cell Biol. 13:4976.
  3. Nagata, K. et al. (1996) J. Biol. Chem. 271:30022.
  4. Sather, S. et al. (2007) Blood 109:1026.
  5. Ekman, C. et al. (2010) J. Thromb. Haemost. 8:838.
  6. Hasanbasic, I. et al. (2005) J. Thromb. Haemost. 3:2790.
  7. Morizono, K. et al. (2011) Cell Host Microbe 9:286.
  8. Gould, W.R. et al. (2005) J. Thromb. Haemost. 3:733.
  9. Robins, R.S. et al. (2012) Blood 121:692.
  10. Alciato, F. et al. (2010) J. Leukoc. Biol. 87:869.
  11. Stenhoff, J. et al. (2004) Biochem. Biophys. Res. Commun. 319:871.
  12. Li, R. et al. (1996) J. Neurosci. 16:2012.
  13. Nakano, T. et al. (1995) J. Biol. Chem. 270:5702.
  14. Caraux, A. et al. (2006) Nat. Immunol. 7:747.
  15. Gallicchio, M. et al. (2005) Blood 105:1970.
  16. Loges, S. et al. (2010) Blood 115:2264.
  17. Gustafsson, A. et al. (2009) PLoS ONE 4:e7575.
Long Name
Growth-arrest-specific Protein 6
Entrez Gene IDs
2621 (Human); 14456 (Mouse)
Alternate Names
AXLLG; AXLLGAXL stimulatory factor; AXSFAXL receptor tyrosine kinase ligand; DKFZp666G247; FLJ34709; Gas6; GAS-6; growth arrest-specific 6; growth arrest-specific protein 6

Citation for Recombinant Mouse Gas6 (Full Length) Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Growth Arrest-Specific 6 Enhances the Suppressive Function of CD4(+)CD25(+) Regulatory T Cells Mainly through Axl Receptor
    Authors: GJ Zhao, JY Zheng, JL Bian, LW Chen, N Dong, Y Yu, GL Hong, A Chandoo, YM Yao, ZQ Lu
    Mediators Inflamm, 2017;2017(0):6848430.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

FAQs

  1. Is the Recombinant Mouse Gas6 (Full Length), CF (R&D Systems catalog # 8310-GS) gamma-carboxylated?

    • R&D Systems does not generally test for the presence of post translational modifications and has not confirmed if this protein is gamma-carboxylated. However, this protein is expressed in mammalian cells (NS0-cells), therefore it is possible that post translational modifications like the Gamma-carboxyglutamate will have occurred. This protein is biologically active, contains the Gla-domain, and is cultured in the presence of vitamin K, so it is expected to have a gamma-carboxylated amino terminus.

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Recombinant Mouse Gas6 (Full Length) Protein, CF
By Anonymous on 05/12/2016
Application: In vitro bioactivity in cell culture