hh Recombinant Mouse Gas6 Protein: R&D Systems

Recombinant Mouse Gas6 (Full Length) Protein, CF

(1 citations)
(1 Review)
  
  • Purity
    >95%, by SDS-PAGE with silver staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to down regulate the expression of Axl in DU145 human prostate carcinoma cells. Mishra, A. et al. (2012) Mol. Cancer Res. 10:703. The ED50 for this effect is 20-100 ng/mL.
  • Source
    Mouse myeloma cell line, NS0-derived Ala46-Pro673, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Ala46 & Tyr47
  • Predicted Molecular Mass
    71 kDa
  • SDS-PAGE
    78-94 kDa, reducing conditions
8310-GS
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Mouse Gas6 (Catalog # 8310-GS) down regulates the expression of Axl in DU145 human prostate carcinoma cells. The ED50 for this effect is 20-100 ng/mL.
1 μg/lane ofRecombinant Mouse Gas6 (Full Length, Catalog # 8310-GS) was resolved with SDS-PAGEunder reducing (R) and non-reducing (NR) conditions and visualized by silverstaining, showing R bands at 85.4 and 69.2 kDa and NR band at 64.4 kDa.
Background: Gas6
Gas6 (Growth Arrest Specific 6) is a secreted 75 kDa multimodular protein that is up-regulated by a wide variety of cell types in response to growth arrest. It is expressed by endothelial cells, fibroblasts, neurons, smooth muscle cells, and platelets, and it plays a role in vascular, thrombotic, atherosclerotic, inflammatory, autoimmune, renal, and cancer pathologies (1, 2). Both Gas6 and the related Protein S contain an extensively gamma -carboxylated N-terminal Gla domain, four EGF-like repeats, and two C-terminal Laminin G-like domains. And like Protein S, Gas6 is dependent upon vitamin K for activity. Within the Gla, EGF-like, and Laminin G-like domains, mouse Gas6 shares 83%, 94%, and 41% aa sequence identity with the equivalent regions in human Gas6, rat Gas6, and mouse Protein S, respectively. Gas6 binds to and induces signaling through the receptor tyrosine kinase TAM subfamily, which includes Axl, Dtk/Tyro3, and Mer (3). Shed soluble forms of Axl and Mer retain the ability to bind Gas6 and function as decoy receptors (4, 5). Gas6 participates in tissue homeostasis by protecting cells from stress-induced apoptosis and promoting apoptotic cell phagocytosis. The affinity of the gamma -carboxylated Gla domain for phosphatidylserine contributes to the role of Gas6 in phagocytosis as well as the cellular entry of select viruses (6, 7). Gas6 can function as a pro-inflammatory molecule by promoting platelet activation (8, 9) and can also inhibit inflammatory cytokine production from monocytes, macrophages, and microglia (10). In addition, Gas6 induces the proliferation of cardiac fibroblasts, Schwann cells, vascular smooth muscle cells, and the differentiation of NK cell precursors (11-14). It also inhibits VEGF-induced angiogenesis (15) and can have either positive or negative effects on tumor cell proliferation and invasion (16, 17).
  • References:
    1. Laurance, S. et al. (2012) Adv. Nutr. 3:196.
    2. Manfioletti, G. et al. (1993) Mol. Cell Biol. 13:4976.
    3. Nagata, K. et al. (1996) J. Biol. Chem. 271:30022.
    4. Sather, S. et al. (2007) Blood 109:1026.
    5. Ekman, C. et al. (2010) J. Thromb. Haemost. 8:838.
    6. Hasanbasic, I. et al. (2005) J. Thromb. Haemost. 3:2790.
    7. Morizono, K. et al. (2011) Cell Host Microbe 9:286.
    8. Gould, W.R. et al. (2005) J. Thromb. Haemost. 3:733.
    9. Robins, R.S. et al. (2012) Blood 121:692.
    10. Alciato, F. et al. (2010) J. Leukoc. Biol. 87:869.
    11. Stenhoff, J. et al. (2004) Biochem. Biophys. Res. Commun. 319:871.
    12. Li, R. et al. (1996) J. Neurosci. 16:2012.
    13. Nakano, T. et al. (1995) J. Biol. Chem. 270:5702.
    14. Caraux, A. et al. (2006) Nat. Immunol. 7:747.
    15. Gallicchio, M. et al. (2005) Blood 105:1970.
    16. Loges, S. et al. (2010) Blood 115:2264.
    17. Gustafsson, A. et al. (2009) PLoS ONE 4:e7575.
  • Long Name:
    Growth-arrest-specific Protein 6
  • Entrez Gene IDs:
    2621 (Human); 14456 (Mouse)
  • Alternate Names:
    AXLLG; AXLLGAXL stimulatory factor; AXSFAXL receptor tyrosine kinase ligand; DKFZp666G247; FLJ34709; Gas6; GAS-6; growth arrest-specific 6; growth arrest-specific protein 6
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Growth Arrest-Specific 6 Enhances the Suppressive Function of CD4(+)CD25(+) Regulatory T Cells Mainly through Axl Receptor
    Authors: GJ Zhao, JY Zheng, JL Bian, LW Chen, N Dong, Y Yu, GL Hong, A Chandoo, YM Yao, ZQ Lu
    Mediators Inflamm, 2017;2017(0):6848430.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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ApplicationIn vitro bioactivity in cell culture

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