Recombinant Mouse IL-10 Protein

(35 citations)
(2 Reviews)
  
  • Purity
    >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using MC/9‑2 mouse mast cells. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. The ED50 for this effect is 0.1‑0.6 ng/mL.
  • Source
    E. coli-derived Ser19-Ser178
  • Accession #
  • N-terminal Sequence
    Analysis
    Ser19
  • Structure / Form
    Noncovalently-linked homodimer
  • Predicted Molecular Mass
    18 kDa
  • SDS-PAGE
    17 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
417-ML
 
417-ML/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Mouse IL-10 (Catalog # 417‑ML) stimulates cell proliferation of the MC/9‑2 mouse mast cell line. The ED50 for this effect is 0.1-0.6 ng/mL.
1 μg/lane of Recombinant Mouse IL-10 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.
Background: IL-10

Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of alpha -helical cytokines that also includes IL‑19, IL‑20, IL-22, and IL-24 (1, 2). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts (2-5). Mature mouse IL-10 shares 85% amino acid sequence identity with rat and 70%-77% with bovine, canine, equine, feline, human, ovine, and porcine IL-10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6, 7). IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer (8-10). The IL-10 dimer binds to two IL-10 R alpha /IL-10 R1 chains, resulting in recruitment of two IL-10 R beta /IL-10 R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 (11). IL-10 R beta does not bind IL-10 by itself but is required for signal transduction (1). IL-10 R beta also associates with IL‑20 R alpha, IL-22 R alpha, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL‑29 (12-14). IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation (15-17). It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses (2).

  • References:
    1. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
    2. O’Garra, A. and P. Vieira (2007) Nat. Rev. Immunol. 7:425.
    3. Mathurin, P. et al. (2002) Am. J. Physiol. Gastrointest. Liver Physiol. 282:G981.
    4. Grewe, M. et al. (1995) J. Invest. Dermatol. 104:3.
    5. Szony, B.J. et al. (1999) Mol. Hum. Reprod. 5:1059.
    6. Vieira, P. et al. (1991) Proc. Natl. Acad. Sci. 88:1172.
    7. Hsu, D.-H. et al. (1990) Science 250:830.
    8. Moore, K. et al. (1990) Science 248:1230.
    9. Windsor, W.T. et al. (1993) Biochemistry 32:8807.
    10. Syto, R. et al. (1998) Biochemistry 37:16943.
    11. Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
    12. Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
    13. Hor, S. et al. (2004) J. Biol. Chem. 279:33343.
    14. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
    15. Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.
    16. Wu, K. et al. (2007) Cell. Mol. Immunol. 4:269.
    17. Blackburn, S.D. and E.J. Wherry (2007) Trends Microbiol. 15:143.
  • Long Name:
    Interleukin 10
  • Entrez Gene IDs:
    3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
  • Alternate Names:
    CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

35 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Proof-of-Concept Gene Editing for the Murine Model of Inducible Arginase-1 Deficiency
    Authors: YY Sin, PR Price, LL Ballantyne, CD Funk
    Sci Rep, 2017;7(1):2585.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  2. IL-10 down-regulates the expression of survival associated gene hspX of Mycobacterium tuberculosis in murine macrophage
    Authors: B Jee, P Sharma, K Katoch, B Joshi, SK Awasthi
    Braz J Infect Dis, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  3. PEDF mediates pathological neovascularization by regulating macrophage recruitment and polarization in the mouse model of oxygen-induced retinopathy
    Authors: S Gao, C Li, Y Zhu, Y Wang, A Sui, Y Zhong, B Xie, X Shen
    Sci Rep, 2017;7(0):42846.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  4. Novel mechanism of macrophage-mediated metastasis revealed in a zebrafish model of tumor development.
    Authors: Wang J, Cao Z, Zhang X, Nakamura M, Sun M, Hartman J, Harris R, Sun Y, Cao Y
    Cancer Res, 2015;75(2):306-15.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  5. Adenosine 5'-monophosphate-activated protein kinase regulates IL-10-mediated anti-inflammatory signaling pathways in macrophages.
    Authors: Zhu Y, Brown J, Sag D, Zhang L, Suttles J
    J Immunol, 2015;194(2):584-94.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  6. miR-20b suppresses Th17 differentiation and the pathogenesis of experimental autoimmune encephalomyelitis by targeting RORgammat and STAT3.
    Authors: Zhu E, Wang X, Zheng B, Wang Q, Hao J, Chen S, Zhao Q, Zhao L, Wu Z, Yin Z
    J Immunol, 2014;192(12):5599-609.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  7. Wound administration of M2-polarized macrophages does not improve murine cutaneous healing responses.
    Authors: Jetten, Nadine, Roumans, Nadia, Gijbels, Marion J, Romano, Andrea, Post, Mark J, de Winther, Menno P, van der Hulst, Rene R W, Xanthoulea, Sofia
    PLoS ONE, 2014;9(7):e102994.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  8. TLR9 ligands induce S100A8 in macrophages via a STAT3-dependent pathway which requires IL-10 and PGE2.
    Authors: Hsu, Kenneth, Chung, Yuen Min, Endoh, Yasumi, Geczy, Carolyn
    PLoS ONE, 2014;9(8):e103629.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  9. Axl receptor blockade ameliorates pulmonary pathology resulting from primary viral infection and viral exacerbation of asthma.
    Authors: Shibata T, Habiel D, Coelho A, Kunkel S, Lukacs N, Hogaboam C
    J Immunol, 2014;192(8):3569-81.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: Luminex standard
  10. GM-CSF treated F4/80+ BMCs improve murine hind limb ischemia similar to M-CSF differentiated macrophages.
    Authors: Kuwahara G, Nishinakamura H, Kojima D, Tashiro T, Kodama S
    PLoS ONE, 2014;9(9):e106987.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  11. TGF-beta signaling initiated in dendritic cells instructs suppressive effects on Th17 differentiation at the site of neuroinflammation.
    Authors: Speck S, Lim J, Shelake S, Matka M, Stoddard J, Farr A, Kuchroo V, Laouar Y
    PLoS ONE, 2014;9(7):e102390.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Standard
  12. Thioredoxin-interacting protein gene expression via MondoA is rapidly and transiently suppressed during inflammatory responses.
    Authors: Kanari Y, Sato Y, Aoyama S, Muta T
    PLoS ONE, 2013;8(3):e59026.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  13. IL-4/IL-13-dependent and independent expression of miR-124 and its contribution to M2 phenotype of monocytic cells in normal conditions and during allergic inflammation.
    Authors: Veremeyko, Tatyana, Siddiqui, Shafiudd, Sotnikov, Ilya, Yung, Amanda, Ponomarev, Eugene D
    PLoS ONE, 2013;8(12):e81774.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  14. Regulation of innate CD8+ T-cell activation mediated by cytokines.
    Proc Natl Acad Sci U S A, 2012;109(25):9971-6.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  15. WNT5A Signaling Contributes to Abeta-Induced Neuroinflammation and Neurotoxicity.
    Authors: Li B, Zhong L, Yang X, Andersson T, Huang M, Tang SJ
    PLoS ONE, 2011;6(8):e22920.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  16. Essential role for IL-27 receptor signaling in prevention of Th1-mediated immunopathology during malaria infection.
    Authors: Findlay EG, Greig R, Stumhofer JS
    J. Immunol., 2010;185(4):2482-92.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  17. Interleukin-1 family cytokines as mucosal vaccine adjuvants for induction of protective immunity against influenza virus.
    Authors: Kayamuro H, Yoshioka Y, Abe Y, Arita S, Katayama K, Nomura T, Yoshikawa T, Kubota-Koketsu R, Ikuta K, Okamoto S, Mori Y, Kunisawa J, Kiyono H, Itoh N, Nagano K, Kamada H, Tsutsumi Y, Tsunoda S
    J. Virol., 2010;84(24):12703-12.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  18. ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis.
    Authors: Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T
    Microbes Infect., 2009;11(4):524-30.
  19. Role of interleukin 10 in murine postoperative ileus.
    Authors: Stoffels B, Schmidt J, Nakao A, Nazir A, Chanthaphavong RS, Bauer AJ
    Gut, 2009;58(5):648-60.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  20. The water channel aquaporin 1 is a novel molecular target of polychlorinated biphenyls for in utero anomalies.
    Authors: Tewari N, Kalkunte S, Murray DW, Sharma S
    J. Biol. Chem., 2009;284(22):15224-32.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  21. Tolerogenic signals delivered by dendritic cells to T cells through a galectin-1-driven immunoregulatory circuit involving interleukin 27 and interleukin 10.
    Authors: Ilarregui JM, Croci DO, Bianco GA, Toscano MA, Salatino M, Vermeulen ME, Geffner JR, Rabinovich GA
    Nat. Immunol., 2009;10(9):981-91.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  22. Adverse functions of IL-17A in experimental sepsis.
    Authors: Flierl MA, Rittirsch D, Gao H, Hoesel LM, Nadeau BA, Day DE, Zetoune FS, Sarma JV, Huber-Lang MS, Ferrara JL, Ward PA
    FASEB J., 2008;22(7):2198-205.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  23. CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis.
    Authors: Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T
    Immunology, 2008;125(2):272-9.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  24. IL-2 is essential for TGF-beta to convert naive CD4+CD25- cells to CD25+Foxp3+ regulatory T cells and for expansion of these cells.
    Authors: Zheng SG, Wang J, Wang P, Gray JD, Horwitz DA
    J. Immunol., 2007;178(4):2018-27.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  25. Dendritic cells loaded with apoptotic antibody-coated tumor cells provide protective immunity against B-cell lymphoma in vivo.
    Authors: Franki SN, Steward KK, Betting DJ, Kafi K, Yamada RE, Timmerman JM
    Blood, 2007;111(3):1504-11.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  26. Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
    Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG
    J. Immunol., 2006;177(3):1967-74.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  27. Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors.
    Authors: Hajjar AM, Harvey MD, Shaffer SA, Goodlett DR, Sjostedt A, Edebro H, Forsman M, Bystrom M, Pelletier M, Wilson CB, Miller SI, Skerrett SJ, Ernst RK
    Infect. Immun., 2006;74(12):6730-8.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  28. Severe sepsis exacerbates cell-mediated immunity in the lung due to an altered dendritic cell cytokine profile.
    Authors: Wen H, Hogaboam CM, Gauldie J, Kunkel SL
    Am. J. Pathol., 2006;168(6):1940-50.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  29. In vivo IL-10 gene delivery attenuates bleomycin induced pulmonary fibrosis by inhibiting the production and activation of TGF-beta in the lung.
    Authors: Nakagome K, Dohi M, Okunishi K, Tanaka R, Miyazaki J, Yamamoto K
    Thorax, 2006;61(10):886-94.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  30. Sequential ELISA to profile multiple cytokines from small volumes.
    Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
    J. Immunol. Methods, 2005;302(1):172-81.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  31. IFN-alpha priming results in a gain of proinflammatory function by IL-10: implications for systemic lupus erythematosus pathogenesis.
    Authors: Sharif MN, Tassiulas I, Hu Y, Mecklenbrauker I, Tarakhovsky A, Ivashkiv LB
    J. Immunol., 2004;172(10):6476-81.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  32. IL-22 increases the innate immunity of tissues.
    Authors: Wolk K, Kunz S, Witte E, Friedrich M, Asadullah K, Sabat R
    Immunity, 2004;21(2):241-54.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  33. Selective macrophage suppression during sepsis.
    Authors: Ellaban E, Bolgos G, Remick D
    Cell. Immunol., 2004;231(1):103-11.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  34. Conversion of peripheral CD4+CD25- naive T cells to CD4+CD25+ regulatory T cells by TGF-beta induction of transcription factor Foxp3.
    Authors: Chen, WanJun, Jin, Wenwen, Hardegen, Neil, Lei, Ke-Jian, Li, Li, Marinos, Nancy, McGrady, George, Wahl, Sharon M
    J Exp Med, 2003;198(12):1875-86.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  35. Human macrophages induce CD4(+)Foxp3(+) regulatory T cells via binding and re-release of TGF-beta.
    Authors: Schmidt A, Zhang X, Joshi R, Iqbal S, Wahlund C, Gabrielsson S, Harris R, Tegner J
    Immunol Cell Biol, 0;94(8):747-62.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
Expand to show all 35 Citations
Average Rating: 4 (Based on 2 reviews)

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Immunoassay Standard Recombinant Mouse IL-10 Protein 417-ML
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 Immunoassay Standard Anonymous 11/27/2017
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Immunoassay Standard Recombinant Mouse IL-10 Protein 417-ML
Immunoassay Standard: Recombinant Mouse IL-10 Protein [417-ML]

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ApplicationImmunoassay Standard
  Very Good
 Stem/Immune cell maintenance or differentiation Anonymous 08/02/2016
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ApplicationStem/Immune cell maintenance or differentiation

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