Recombinant Mouse IL-13 R alpha 2 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
539-IR-100
Product Details
Citations (10)
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Recombinant Mouse IL-13 R alpha 2 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit IL-13-dependent proliferation of TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.01‑0.03 µg/mL in the presence of 8 ng/mL of recombinant mouse IL-13.
Source
Mouse myeloma cell line, NS0-derived mouse IL-13 R alpha 2 protein
Mouse IL-13 R alpha 2
(Leu22 - Lys334)
Accession # O88786
IEGRDID Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Leu22
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
63 kDa (monomer)
SDS-PAGE
85-90 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

539-IR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

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Background: IL-13 R alpha 2

Interleukin-13 Receptor alpha 2 (IL-13 Ra2), also known as IL-13 Ra’, IL-13 binding protein, and CD213a2, is a widely expressed 55 kDa cytokine receptor that plays an important role in the Th2‑polarized immune responses characteristic of a variety of pathologies including parasitic infections and allergic asthma (1, 2). Mature mouse IL-13 Ra2 consists of a 313 amino acid (aa) extracellular domain with three fibronectin type-III domains, a WSxWS motif, a 21 aa transmembrane segment, and a 28 aa cytoplasmic domain (3). Within the ECD, mouse IL-13 Ra2 shares 64% and 94% aa sequence identity with human and rat IL-13 Ra2, respectively. A 40 kDa ‑ 50 kDa soluble form of mouse IL-13 Ra2 can be generated by alternate splicing or MMP-8 mediated shedding (4, 5). The biological effects of IL-13 and IL-4 are closely related in part due to a shared receptor system. IL‑13 binds to IL-13 Ra1 which then forms a signaling complex with IL-4 Ra (6, 7). IL-13 Ra2 functions as a decoy receptor by binding and internalizing IL-13 and preventing it from signaling through the IL-13 Ra1/IL-4 Ra complex (3, 8). IL‑13 Ra2 can also block IL-4 induced responses by inhibiting IL-4 bound IL-13 Ra1/IL-4 Ra receptor complexes even though it does not itself bind IL-4 (9, 10). Aside from its decoy function, IL‑13‑activated IL-13 Ra2 directly promotes the development of tissue fibrosis by inducing the transcription of TGF-beta (11). Soluble IL‑13 Ra2 retains ligand binding capability and attenuates responses to IL-13 but not to IL-4 (9, 12). The up‑regulation of transmembrane and soluble IL‑13 Ra2 during Th2-biased immune responses limits the extent of those responses (13 ‑ 15). IL‑13 Ra2 is expressed in some cancers, and its ability to block IL-13 and IL-4 contributes to tumorigenesis and metastasis (10, 16).

References
  1. Wynn, T.A. (2003) Annu. Rev. Immunol. 21:425.
  2. Tabata, Y. et al. (2007) Curr. Allergy Asthma Rep. 7:338.
  3. Donaldson, D.D. et al. (1998) J. Immunol. 161:2317.
  4. Tabata, Y. et al. (2006) J. Immunol. 177:7905.
  5. Chen, W. et al. (2008) J. Allergy Clin. Immunol. 122:625.
  6. Andrews, A.-L. et al. (2006) J. Immunol. 176:7456.
  7. Zurawski, S.M. et al. (1995) J. Biol. Chem. 270:13869.
  8. Caput, D. et al. (1996) J. Biol. Chem. 271:16921.
  9. Andrews, A.-L. et al. (2006) J. Allergy Clin. Immunol. 118:858.
  10. Rahaman, S.O. et al. (2002) Cancer Res. 62:1103.
  11. Fichtner-Feigl, S. et al. (2006) Nat. Med. 12:99.
  12. Zhang, J.G. et al. (1997) J. Biol. Chem. 272:9474.
  13. Chiaramonte, M.G. et al. (2003) J. Exp. Med. 197:687.
  14. Morimoto, M. et al. (2009) J. Immunol. 183:1934.
  15. Zheng, T. et al. (2008) J. Immunol. 180:522.
  16. Fujisawa, T. et al. (2009) Cancer Res. 69:8678.
Long Name
Interleukin 13 Receptor alpha 2
Entrez Gene IDs
3598 (Human); 16165 (Mouse)
Alternate Names
cancer/testis antigen 19; CD213a2 antigen; CD213a2; CT19; IL-13 R alpha 2; IL-13 receptor subunit alpha-2; IL13BP; IL13R alpha 2; IL-13R subunit alpha-2; IL13R; IL-13R; IL13RA2; IL-13Ra2; IL-13R-alpha-2; interleukin 13 binding protein; interleukin 13 receptor alpha 2 chain; interleukin 13 receptor, alpha 2; interleukin-13 receptor subunit alpha-2; Interleukin-13-binding protein

Citations for Recombinant Mouse IL-13 R alpha 2 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Diesel exhaust particles induce cysteine oxidation and s-glutathionylation in house dust mite induced murine asthma.
    Authors: Lee, Gerald B, Brandt, Eric B, Xiao, Chang, Gibson, Aaron M, Le Cras, Timothy, Brown, Lou Ann, Fitzpatrick, Anne M, Khurana Hershey, Gurjit K
    PLoS ONE, 2013;8(3):e60632.
    Species: Mouse
    Sample Types: BALF
    Applications: Bioassay
  2. Interleukin-13 induces T helper type 2 immune responses in OVA-immunized BALB/c mice bearing a T cell lymphoma.
    Authors: Deepak P, Acharya A
    Scand. J. Immunol., 2012;75(1):85-95.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  3. A novel combination immunotherapy for cancer by IL-13Ralpha2-targeted DNA vaccine and immunotoxin in murine tumor models.
    Authors: Nakashima H, Terabe M, Berzofsky JA, Husain SR, Puri RK
    J. Immunol., 2011;187(10):4935-46.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA (Capture)
  4. IL-13 from Th2-type cells suppresses induction of antigen-specific Th1 immunity in a T-cell lymphoma.
    Authors: Deepak P, Kumar S, Kishore D, Acharya A
    Int. Immunol., 2010;22(1):53-63.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  5. Matrix metalloproteinase 8 contributes to solubilization of IL-13 receptor alpha2 in vivo.
    Authors: Chen W, Tabata Y, Gibson AM, Daines MO, Warrier MR, Wills-Karp M, Hershey GK
    J. Allergy Clin. Immunol., 2008;122(3):625-32.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  6. B7-DC induced by IL-13 works as a feedback regulator in the effector phase of allergic asthma.
    Authors: Matsumoto K, Fukuyama S, Eguchi-Tsuda M, Nakano T, Matsumoto T, Matsumura M, Moriwaki A, Kan-o K, Wada Y, Yagita H, Shin T, Pardoll DM, Patcharee R, Azuma M, Nakanishi Y, Inoue H
    Biochem. Biophys. Res. Commun., 2007;365(1):170-5.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  7. IL-13Ralpha2 and IL-10 coordinately suppress airway inflammation, airway-hyperreactivity, and fibrosis in mice.
    Authors: Wilson MS, Elnekave E, Mentink-Kane MM, Hodges MG, Pesce JT, Ramalingam TR, Thompson RW, Kamanaka M, Flavell RA, Keane-Myers A, Cheever AW, Wynn TA
    J. Clin. Invest., 2007;117(10):2941-51.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  8. Interleukin-13-induced type II polarization of inflammatory macrophages is mediated through suppression of nuclear factor-kappaB and preservation of IkappaBalpha in a T cell lymphoma.
    Authors: Deepak P, Kumar S, Acharya A
    Clin. Exp. Immunol., 2007;149(2):378-86.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  9. T helper 1 cells stimulated with ovalbumin and IL-18 induce airway hyperresponsiveness and lung fibrosis by IFN-gamma and IL-13 production.
    Authors: Hayashi N, Yoshimoto T, Izuhara K, Matsui K, Tanaka T, Nakanishi K
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(37):14765-70.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  10. IL-13 receptor alpha2 promotes epithelial cell regeneration from radiation-induced small intestinal injury in mice.
    Authors: Kawashima R, Kawamura YI, Kato R, Mizutani N, Toyama-Sorimachi N, Dohi T
    Gastroenterology, 2006;131(1):130-41.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)

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